Untersuchungen �ber Wachstum und Speicherstoffsynthese von Hydrogenomonas

Wilde, Erika

doi:10.1007/bf00406429

Cited by 148 publications

(42 citation statements)

References 34 publications

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“…Alcaligenes eutrophus H 16 (Wilde, 1962) ATCC 17699, DSM428, was grown in a mineral medium according to . For heterotrophic growth the organic substrates were added (acids as Na-salts) to a concentration of 0.5 % (acetate to 0.2 %).…”

Section: Growth Conditionsmentioning

confidence: 99%

“…In any case the rates of gas uptake were higher in the presence of methylene blue (either alone or m the presence of oxygen) than with oxygen as the sole hydrogen acceptor. The oxidation of hydrogen by the particulate hydrogen oxidase system or by the soluble hydrogenase and NADH-oxidase was apparently lira-ited by the O2-terminal components of the respiratory chain (Wilde, 1962).…”

Section: Characterization Of Mutantsmentioning

confidence: 99%

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Mutants of Alcaligenes eutrophus defective in autotrophic metabolism

Schink

Schlegel

1978

Arch. Microbiol.

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Abstract. Forty-four mutants of Alcaligenes eutrophusH 16 were isolated which grew poorly or not at all under autotrophic conditions. Four types were characterized with respect to their defects and their physiological properties. One mutant lacked both enzymes specific for autotrophic CO 2 fixation, another one lacked both hydrogenases, and two mutants lacked either the membrane-bound or the soluble hydrogenase.Comparing the results of studies on these mutant types, the following conclusions were drawn: the lack of each hydrogenase enzyme could be partially compensated by the other one; the lack of membrane-bound hydrogenase did not affect autotrophic growth, whereas the lack of the soluble hydrogenase resulted in a decreased autotrophic growth rate. When pyruvate as well as hydrogen were supplied to the wild-type, the cell yield was higher than in the presence ofpyruvate alone. Mutant experiments under these conditions indicated that either of both hydrogenases was able to add to the energy supply of the cell. Only the soluble hydrogenase was involved in the control of the rate of hydrogen oxidation by carbon dioxide; the mutant lacking this enzyme did not respond to the presence or absence of CO 2. The suppression of growth on fructose by hydrogen could be mediated by either of both hydrogenases alone.

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Section: Growth Conditionsmentioning

confidence: 99%

Section: Characterization Of Mutantsmentioning

confidence: 99%

Mutants of Alcaligenes eutrophus defective in autotrophic metabolism

Schink

Schlegel

1978

Arch. Microbiol.

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“…This strain was isolated from a spring near Göttingen about 50 years ago (Schlegel et al, 1961;Walde, 1962) and is probably the best-examined microorganism with regard to polyhydroxyalkanoate (PHA) metabolism. R. eutropha H16 possesses a remarkable metabolic versatility; the strain can use organic compounds, preferably organic acids, as well as molecular H 2 as electron donors, and it can shift between heterotrophic and autotrophic growth modes.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide transcriptome analyses of the ‘Knallgas’ bacterium Ralstonia eutropha H16 with regard to polyhydroxyalkanoate metabolism

et al. 2010

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Ralstonia eutropha H16 is probably the best-studied 'Knallgas' bacterium and producer of poly(3-hydroxybutyrate) (PHB). Genome-wide transcriptome analyses were employed to detect genes that are differentially transcribed during PHB biosynthesis. For this purpose, four transcriptomes from different growth phases of the wild-type H16 and of the two PHB-negative mutants PHB " 4 and DphaC1 were compared: (i) cells from the exponential growth phase with cells that were in transition to stationary growth phase, and (ii) cells from the transition phase with cells from the stationary growth phase of R. eutropha H16, as well as (iii) cells from the transition phase of R. eutropha H16 with those from the transition phase of R. eutropha PHB " 4 and (iv) cells from the transition phase of R. eutropha DphaC1 with those from the transition phase of R. eutropha PHB " 4. Among a large number of genes exhibiting significant changes in transcription level, several genes within the functional class of lipid metabolism were detected. In strain H16, phaP3, accC2, fabZ, fabG and H16_A3307 exhibited a decreased transcription level in the stationary growth phase compared with the transition phase, whereas phaP1, H16_A3311, phaZ2 and phaZ6 were found to be induced in the stationary growth phase. Compared with PHB " 4, we found that phaA, phaB1, paaH1, H16_A3307, phaP3, accC2 and fabG were induced in the wildtype, and phaP1, phaP4, phaZ2 and phaZ6 exhibited an elevated transcription level in PHB " 4. In strain DphaC1, phaA and phaB1 were highly induced compared with PHB " 4. Additionally, the results of this study suggest that mutant strain PHB " 4 is defective in PHB biosynthesis and fatty acid metabolism. A significant downregulation of the two cbb operons in mutant strain PHB " 4 was observed. The putative polyhydroxyalkanoate (PHA) synthase phaC2 identified in strain H16 was further investigated by several functional analyses. Mutant PHB " 4 could be phenotypically complemented by expression of phaC2 from a plasmid; on the other hand, in the mutant H16DphaC1, no PHA production was observed. PhaC2 activity could not be detected in any experiment.

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“…These strains were subsequently lost; besides, the descriptions by Grohmann (1924) suggest that some of them at least could have been mixed cultures. Further attempts to isolate mesophilic, spore-forming, hydrogen-oxidizing bacteria have so far failed (Wilde, 1962 ;Eberhardt, 1965). Thermophilic, hydrogen-oxidizing bacteria that have been described to date belong to the genera Pseudomonas and Flavobacterium (Aragno & Schlegel, 1980).…”

Section: Introductionmentioning

confidence: 99%