Untersuchungen mit J131-markiertem ?-Globulin zur Frage der Abstammung der Liquoreiweissk�rper

Frick, E.; Scheid-Seydel, L.

doi:10.1007/bf01485232

Cited by 153 publications

(28 citation statements)

References 13 publications

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“…An increase of the y-globulin fraction of CSF in neurosyphilis is well known (Kabat, Landow, and Moore, 1942;Kabat, Moore, and Landow, 1942;Schultze and Heremans, 1966). Frick and Scheid-Seydel (1958) found some evidence that the increase of y-globulin in CSF is due to a local production within the central nervous system.…”

Section: Neurosyphilismentioning

confidence: 88%

Immunoglobulins of cerebrospinal fluid in syphilis.

Oxelius¹,

Rorsman²,

Laurell³

1969

Sexually Transmitted Infections

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Section: Neurosyphilismentioning

confidence: 88%

Immunoglobulins of cerebrospinal fluid in syphilis.

Oxelius¹,

Rorsman²,

Laurell³

1969

Sexually Transmitted Infections

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“…Such selective increases of CSF IgG with restricted heterogeneity are found in about 95% of MS patients (1). Kinetic studies of IgG turnover within the central nervous system (CNS), the CSF-IgG index, and comparative CSFserum K/A chain ratio studies all argue strongly in favor of intrathecal synthesis of the oligoclonal IgG in MS (2)(3)(4)(5)(6). The IgG patterns differ from one patient to another but remain fairly constant in each individual during the course of the disease (7)(8)(9)(10) and do not appear to be affected by treatment with immunomodulatory drugs (11).…”

mentioning

confidence: 99%

Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries.

Cortese¹,

Tafi²,

Grimaldi³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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The study of the origin and pathogenetic relevance of the oligoclonal antibodies present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients has been hampered by a lack of specific ligands. We recently reported a general strategy, based on phage-displayed random peptide libraries, to identify ligands for disease-specific antibodies even in the absence of any information on the nature of the pathologic antigen. With this procedure, we identified several peptides specifically recognized by antibodies present in the CSF of MS patients. Using these peptides as reagents, we demonstrated that they mimic different natural epitopes and react with antibodies enriched in the CSF of MS patients.Antibodies recognizing the selected peptides are commonly found with equal frequency in the sera of MS patients and of normal individuals. In contrast, the repertoire of CSF antibodies appears to be individual-specific and is probably the result of a nonspecific immunodysregulation rather than a stereotyped response to a single antigen/agent. (2-6). The IgG patterns differ from one patient to another but remain fairly constant in each individual during the course of the disease (7-10) and do not appear to be affected by treatment with immunomodulatory drugs (11). These data seem to imply a specific immune response, developed within the CNS, against persistent epitopes.OB antibodies can also be found in other inflammatory neurological diseases such as tuberculous meningitis, neurosyphilis, progressive rubella panencephalitis, subacute sclerosing panencephalitis, and others (8,(12)(13)(14)(15)(16). In these pathologies, the majority of the oligoclonal IgG has been found to recognize the etiologic agent (17), whereas for MS there is no general consensus on the nature of the antigens that react with the oligoclonal antibodies present in the CSF (18-21). The failure to identify the natural antigens binding to the OB antibodies has brought the study of their origin and pathological relevance to a standstill.Over the last few years, random peptide libraries (RPLs) displayed on phage have been used as a source of ligands to antibodies and receptors. These ligands are not necessarily identical or even similar to the natural ones, but mimic their binding properties. RPLs have been extensively used to select peptides mimicking linear epitopes or folded protein domains, and even nonproteinaceous molecules (22-24). Since the binding properties of the selected peptides to antibodies are usually determined when they are displayed on the phage particle, we have introduced the term phagotope to refer to this special type of phage displayed epitope.Using a pool of sera from rheumatoid arthritis (RA) patients, Dybwad et al. (25) have been able to identify phagotopes displaying a higher frequency of reactivity with antibodies present in RA sera than in normal control sera. Recently, we have developed a novel and general strategy to identify phagotopes that bind to disease-specific antibodies present in the serum of patients th...

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“…Einige neurologische Erkrankungen sind von einer zusätzlichen lokal im ZNS stattfindenden Synthese von Immunglobulin G begleitet (2). Dadurch setzt sich die im Liquor gemessene IgG-Konzentration aus einem Anteil, der aus dem Serum stammt und einem Anteil, der aus dem ZNS stammt, zusammen.…”

Section: Introductionunclassified

Quantitative Bestimmung der lokal im Zentralnervensystem synthetisierten Immunglobulin G-Fraktion des Liquors.

Reiber¹

1979

Clinical Chemistry and Laboratory Medicine

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Zusammenfassung: Für eine Gruppe von n = 334 Kontrollpersonen wurde der Normalbereich der Liquor/SerumQuotienten von Immunglobulin G (IgG) und Albumin bestimmt. Der Korrelationskoeffizient zwischen den beiden Quotienten ist r = 0,71. Die Regressionsgerade ist y = 0,41x + 0,00014. Aus funktioneilen und statistischen Gründen sollte die theoretische Gerade durch den Ursprung gehen. Entsprechend wird dann für das Datenkollektiv y = 0,43 errechnet. Der Vertrauensbereich des IgG-Quotienten (y) für einen gegebenen Albumin-Quotienten (x) ist mit ± 2 s yjc = ± 0,001 charakterisiert (sy. x = Streuung der y-Werte um die Regressionslinie). Diese Auswertung des IgGQuotienten für den individuellen Albumin-Quotienten des jeweiligen Patienten ist die Basis für die empfindliche numerische Bestimmung der lokal im Zentralnervensystem synthetisierten pathologischen IgG-Fraktion (IgG p ):Albumin ( Calculation of the IgG fraction of cerebro spinal fluid locally synthesized in the central nervous systemSummary: The cerebrospinal fluid (CSF)/serum concentration ratios of immunoglobulin G and albumin were determined for a group of n = 334 control patients. The correlation coefficient relating the two quotients was r = 0.71. The regression line was found to be y = 0.41 + Ö.OÖÖ14. For statistical and functional reasons it is plausible that the theoretical line should go through the origin. The corresponding function would than be y = 0.43 x. The confidence range of the IgG quotient (y) for a given albumin quotient (x) is characterised by ± 2 s y . x = ± 0.001 (Sy. x is the standard deviation of the y values from the regression line). If the IgG quotient is evaluated for the individual albumin ratio of the patient, the following formula can be used for calculation of the locally synthesized concentration of pathological IgG (IgGp) in CSF:This method of determination is independent of the variables which are known to influence the individual IgG concentration (and other protein concentrations) in CSF, e. g. age, sex, individual blood brain barrier condition, extraction volume of CSF and the method used for protein determination. In comparison with other methods, particularly with respect to the statistics and biochemistry, the reported formula allows an optimal evaluation of pathological IgG values in CSF.

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Untersuchungen mit J131-markiertem ?-Globulin zur Frage der Abstammung der Liquoreiweissk�rper

Cited by 153 publications

References 13 publications

Immunoglobulins of cerebrospinal fluid in syphilis.

Immunoglobulins of cerebrospinal fluid in syphilis.

Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries.

Quantitative Bestimmung der lokal im Zentralnervensystem synthetisierten Immunglobulin G-Fraktion des Liquors.

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