Untersuchungen zum Gehalt an 2,6-Diaminopimelinsäure (DAP) und das DAP: N-Verhältnis in Pansenbakterien in Abhängigkeit von der Zeit nach der Fütterung

Krawielitzki, R.; Piatkowski, B.; Kreienbring, F.

doi:10.1080/17450397809421011

Cited by 19 publications

(3 citation statements)

References 24 publications

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“…The problems associated with the use of DAPA as a marker for bacterial-N have been extensively reported by (1978). In the present study the mean DAPA-N: TN values for mixed rumen bacterial isolates, apart from those when the urea diet was given, were within the range of values Ling & Buttery (1978) and Krawielitzki et al (1978). The higher value of DAPA-N:TN, found in bacteria isolated from steers given urea, may have been due to the presence of different species of bacteria containing higher levels of DAPA (Work & Dewey, 1953) and/or lower levels of TN.…”

Section: -2supporting

confidence: 58%

The simultaneous estimation of the amounts of protozoal, bacterial and dietary nitrogen entering the duodenum of steers

Cockburn¹,

Williams²

1984

Br J Nutr

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1. Four steers were given straw and tapioca diets, twice daily, in a 4 x 4 Latin-square design. These diets, containing 4.2 g nitrogen/kg dry matter (DM), were further supplemented with either urea, decorticated groundnut meal (DCGM), untreated (UT) casein or formaldehyde-treated (FT) casein to give a total of 19.7 g N/kg DM and 10.5 MJ/kg DM daily.2. Concurrent samples of rumen bacteria and protozoa and abomasal digesta were collected for each period of the experiment and the concentrations of 2-aminoethyl phosphonic acid (AEPA), diaminopimelic acid (DAPA), total nitrogen (TN), total phosphorus (TP), amino acids and hexosamines were determined in the dried preparations. The nature of the dietary supplements had little effect on the concentrations of most of these constituents or on the total protozoal numbers.3. Abomasal digesta samples marked with polyethylene glycol (PEG) and chromic oxide for flow estimation were collected over 24 h, and the proportions of protozoal-N, bacterial-N and microbial-N estimated simultaneously using the markers AEPA, DAPA and RNA respectively. These digesta-N components were also estimated using an amino acid profiling (AAP) method which gave, in addition, estimates of the dietary and endogenous components. For the diets containing casein, the proportion of dietary casein was estimated directly using casein-P as a marker. 5.Giving FT casein significantly (P < 0401) increased the flow of casein-N at the abomasum and a significantly ( P < 0,001) greater proportion of casein-N was found in abomasal NAN (0.5 1 v. 0.09) where FT rather than UT casein was given.6. The AAP method gave results for the proportions of microbial-and dietary-N (where casein was given) which were, in general, slightly lower than those obtained using RNA and casein-P as markers. Agreement with estimates of bacterial protein (from DAPA) and of protozoal protein (from AEPA) was less satisfactory.7. Comparisons of the various estimates of the proportions of microbial-N in abomasal digesta suggested that the results obtained for protozoal-N by AEPA were overestimates. AEPA was found in mixed rumen bacteria which may have accounted in part for these overestimates. However, AEPA was not detected in any of the dietary ingredients.

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Section: -2supporting

confidence: 58%

The simultaneous estimation of the amounts of protozoal, bacterial and dietary nitrogen entering the duodenum of steers

Cockburn¹,

Williams²

1984

Br J Nutr

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“…Trace mineral (TM) solubility and bioavailability were assessed by measuring the trace element concentration in fractions obtained after successive centrifugations of the final fermentation medium, allowing one to separate the remaining big particle fractions (UNSOL; feed particles, insolubilized minerals, protozoa), a fraction enriched in ruminal bacteria (BACT) and a final supernatant (SOL) containing the solubilized minerals. The correct separation of the different fractions via centrifugation was verified through diaminopimelic acid analysis (DAPA) of the UNSOL and BACT [23,24]. For the incubations testing Mn sources, the substrate was incubated solely (CON) or with the addition of 0.06% DM of Mn (as MnO or MnSO 4 ), added at the start (MnO_0 h and MnS_0 h), after 24 h (MnO_24 h and MnS_24 h) or 48 h (MnO_48 h and MnS_48 h) of incubation.…”

Section: Experimental Design and Treatmentsmentioning

confidence: 99%

Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro

Vigh

Criste

Gragnic³

et al. 2023

Agriculture

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The aim of this study was to assess the effects of supplementation with inorganic sources of manganese (MnO, MnSO4), zinc (ZnO, ZnSO4) and copper (CuSO4) at different levels (0.06%DM for Mn, 0.05%DM for Zn; 0.01 and 0.05%DM for Cu) on in vitro rumen fermentation, solubility and bioavailability. Fermentation activity was measured by total gas production (TGP) and dry matter degradability after 70 h of fermentation (dDM%). Trace mineral (TM) solubility was estimated via the TM concentration in the supernatant of the final fermentation medium (SOL) and TM bioavailability from the TM concentration in a bacterial-enriched fraction (BACT). Mn (regardless of source) and ZnO tended (p < 0.10) to decrease, while Cu showed no significant effect on TGP. The addition of inorganic Mn and of ZnO tended (p < 0.10) to decrease, ZnSO4 tended to increase (p < 0.10), whilst Cu showed no effect on dDM%. Concerning solubility, Mn (MnO and MnSO4), ZnSO4 and CuSO4 significantly (p < 0.05, p < 0.001 and p < 0.01) increased, while ZnO did not affect TM content in the SOL. These results indicate that MnSO4, ZnSO4 and CuSO4 are highly soluble, MnO is quite soluble, while ZnO has a low solubility in the rumen. Based on the TM content in BACT, MnO, MnS4 and CuSO4 have high bioavailability, while ZnO is poorly assimilated by rumen bacteria. However, the lack of clear inhibition or improvement in fermentations suggests that the rumen microbiota have a low requirement for TM supplementation.

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“…Trace-mineral (TM) solubility and bioavailability were assessed by measuring the trace element concentration in fractions obtained after successive centrifugations of the final fermentation medium, allowing to separate the remaining big particles fraction (UNSOL; feed particles, insolubilized minerals, protozoa), a fraction enriched in ruminal bacteria (BACT), and a final supernatant (SOL), containing the solubilized minerals. The correct separation of the different fractions by centrifugation was verified by a diaminopimelic acid analysis (DAPA) of the UNSOL and BACT [21,22]. For the incubations testing Mn sources, the substrate was incubated solely (CON), or with an addition of 0.06% DM of Mn (as MnO or MnSO4), added at the start (MnO_0h and MnS_0h), after 24hrs (MnO_24h and MnS_24h) or 48hrs (MnO_48h and MnS_48h) of incubation.…”

Section: Experimental Design and Treatmentsmentioning

confidence: 99%

Ruminal Solubility and Bioavailability of Inorganic Trace-Mineral Sources and Effects on Fermentation Activity Measured In Vitro

Vigh¹,

Criste²,

Gragnic³

et al. 2023

Preprint

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The aim of this study was to assess the effects of supplementation of inorganic sources of manganese (MnO, MnSO4), zinc (ZnO, ZnSO4) and copper (CuSO4) at different levels (0.06%DM for Mn, 0.05%DM for Zn; and 0.01 and 0.05%DM for Cu) on in vitro rumen fermentation, solubility and bioavailability. Fermentation activity was measured by total gas production (TGP) and dry matter degradability after 70hrs of fermentation (dDM%). Trace-mineral (TM) solubility was estimated by the TM concentration in the supernatant of the final fermentation medium (SOL) and TM bioavailability by the TM concentration in a bacterial enriched fraction (BACT). Mn (regardless of source) and ZnO tended (p&lt;0.10) to decrease, while Cu showed no significant effect on TGP. The addition of inorganic Mn and of ZnO tended (p&lt;0.10) to decrease, ZnSO4 tended to increase (p&lt;0.10), whilst Cu showed no effect on dDM%. Concerning solubility, Mn (MnO and MnSO4), ZnSO4 and CuSO4 significantly (p&lt;0.05, p&lt;0.001 and p&lt;0.01) increased, while ZnO did not affect TM content of the SOL. These results indicate that MnSO4, ZnSO4 and CuSO4 are highly soluble, MnO is quite soluble, while ZnO has a low solubility in the rumen. Based on the TM content of BACT, MnO, MnS4 and CuSO4 have a high bioavailability, while ZnO is poorly assimilated by rumen bacteria. However, the lack of clear inhibition or improvement of fermentations suggests that the rumen microbiota has low requirement for TM supplementation.

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Untersuchungen zum Gehalt an 2,6-Diaminopimelinsäure (DAP) und das DAP: N-Verhältnis in Pansenbakterien in Abhängigkeit von der Zeit nach der Fütterung

Cited by 19 publications

References 24 publications

The simultaneous estimation of the amounts of protozoal, bacterial and dietary nitrogen entering the duodenum of steers

The simultaneous estimation of the amounts of protozoal, bacterial and dietary nitrogen entering the duodenum of steers

Ruminal Solubility and Bioavailability of Inorganic Trace Mineral Sources and Effects on Fermentation Activity Measured in Vitro

Ruminal Solubility and Bioavailability of Inorganic Trace-Mineral Sources and Effects on Fermentation Activity Measured In Vitro

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