Untersuchungen zur Haltbarkeit der Ferment-Aktivität im Serum

Amelung, D; Hofmann, L; Otto, L

doi:10.1055/s-0028-1110664

Cited by 9 publications

(1 citation statement)

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“…This is supported by the findings of Sydney et al [24] where storage of human plasma for 4-6 weeks at -90°C did not alter LDH activity. Also, our observation when LDH was stored at -4°C is in accord with the study of [25,26], who described significant instability of LDH activity when stored at -8°C to 10°C. We did not find any evidence to believe that freezethaw of fresh samples of human serum or after storage for 7 days has any harmful effect on the stability of CPK.…”

Section: Discussionsupporting

confidence: 91%

Effect of Freezing-thawing and Storage Time on Some Specific Human Enzymes

Ekweogu

Nwankpa

Emengaha

et al. 2018

ARRB

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The present research was designed and conducted to study the effect of freezing-thawing and storage time on some specific human enzymes. The enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) were analysed immediately after sample collection, after undergoing freeze thaw at -4°C, -20°C and -70°C at day o and after 7 days of storage at -4°C, -20°C and -70°C. A total of 50 healthy males and 50 healthy females were used for the study and sample collection was by pooled serum. Our results show that there was no statistically significant difference (p>0.05) between AST, ALT, ALP and CPK levels obtained after freeze-thaw at -4°C, -20°C and -70°C at day 0 when compared with the control for both males and females. Also, no statistically significant difference (p>0.05) was seen in the levels of AST, ALT, ALP, CPK analysed Original Research Article

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Section: Discussionsupporting

confidence: 91%