Untimely cumulus removal affects ooplasmic competence

Kocent, Justin; Monahan, D.; Neri, Q.V.; Rosenwaks, Z.; Palermo, G.D.

doi:10.1016/j.fertnstert.2010.07.976

Cited by 4 publications

(3 citation statements)

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“…The persistence of immature GV cytoplasmic characteristics in some MII oocytes may be the reason for the observed impaired fertilizability or complete fertilization failure in some women with a large number of the so-called MII oocytes and apparently competent spermatozoa who previously had successful ICSI fertilization (n ¼ 37). In these women, the only factor identified was a significantly shorter time interval between hCG and oocyte decoronization in comparison to their corresponding cycle with fertilization (P< .01) (86).…”

Section: Figurementioning

confidence: 89%

Development and current applications of assisted fertilization

Palermo

Neri

Monahan

et al. 2012

Fertility and Sterility

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Section: Figurementioning

confidence: 89%

Development and current applications of assisted fertilization

Palermo

Neri

Monahan

et al. 2012

Fertility and Sterility

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“…In this manner, the patient provided the study sample (failed fertilization cycles) and its own control (cycles with fertilization). We also compared the study population to patients who achieved fertilization in all oocytes injected considered as the gold standard [98]. …”

Section: How To Diagnose Fertilization Failurementioning

confidence: 99%

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Neri¹,

Lee²,

Rosenwaks³

et al. 2014

Cell Calcium

131

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Summary Since the establishment of in vitro fertilization, it became evident that almost half of the couples failed to achieve fertilization and this phenomenon was attributed to a male gamete dysfunction. The adoption of assisted fertilization techniques particularly ICSI has been able to alleviate male factor infertility by granting the consistent ability of a viable spermatozoon to activate an oocyte. Single sperm injection, by pinpointing the beginning of fertilization, has been an invaluable tool in clarifying the different aspects of early fertilization and syngamy. However, even with ICSI some couples fail to fertilize due to ooplasmic dysmaturity in relation to the achieved nuclear maturation marked by the extrusion of the first polar body. More uncommon are cases where the spermatozoa partially or completely lack the specific oocyte activating factor. In this work, we review the most relevant aspects of fertilization and its failure through assisted reproductive technologies. Attempts at diagnosing and treating clinical fertilization failure are described.

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“…This is not limited to ooplasmic inadequacy that can be corrected by tweaking the superovulation protocol and may also explain the paradoxical higher fertilization rates observed with IVF over ICSI as the overnight exposure to the male gamete allows an unsuitable ootid to reach ooplasmic competence. 7,24,46,47 In one retrospective analysis of 555 couples with failed fertilization after IVF (n ¼ 388) or ICSI (n ¼ 167), couples who opted for ICSI on subsequent cycles achieved a clinical pregnancy rate of 24% compared with 22% for those who used standard insemination. Cases with failed fertilization with ICSI and subsequently inseminated by the same method generated a pregnancy rate of 22%.…”

Section: The Debatementioning

confidence: 99%

To ICSI or Not to ICSI

2015

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Intracytoplasmic sperm injection (ICSI) is the most effective assisted reproductive procedure enabling fertilization in severe forms of male factor indications and male gamete dysfunction. Reliability of ICSI has allowed the expansion of its application to other forms of infertility rendering it the most popular assisted reproduction technology (ART) insemination method worldwide. The concern related to the invasiveness of ICSI together with the arbitrary selection of the inseminating spermatozoon has induced the execution of studies to compare the performance of ICSI in non-male factor infertility with standard in vitro insemination approach. Not surprisingly, the outcome has evidenced that ICSI does not yield higher pregnancy rates than in vitro fertilization but functions invariably as a normalizer of fertilization mollifying the absent or low fertilization. The follow-up studies on ICSI children have evidenced that the procedure is safe and the slightly higher incidences of neonatal malformations or de novo gonosomal abnormalities are related to the genetics of the infertile couples. Furthermore, ICSI is accepted for some specific indications such as low number and poor morphology oocytes, thicker zona, excess polyspermia, PGD/PGS/PGT (preimplantation genetic diagnosis/preimplantation genetic screening/preimplantation genetic testing), discordant HCV/HIV (hepatitis C virus/human immunodeficiency virus) couples, in vitro maturation (IVM), and oocyte cryopreservation. Only the advent of new biomarkers in combination with routine semen analysis capable of identifying the fertilization competence of the spermatozoon can guide the reproductive physician toward the proper insemination method.

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Untimely cumulus removal affects ooplasmic competence

Cited by 4 publications

References 0 publications

Development and current applications of assisted fertilization

Development and current applications of assisted fertilization

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

To ICSI or Not to ICSI

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