2002
DOI: 10.1007/s00412-002-0217-1
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Unusual chromosome cleavage dynamic in rodent neonatal germ cells

Abstract: At the metaphase/anaphase transition in the mouse and rat male germ lines during the perinatal period, sister centromeres separate before sister chromatids. This gives the chromosomes an unusual appearance that resembles the premature centromere division described in some human pathological conditions such as Roberts syndrome. At the same period, there is also an unusual pattern of DNA methylation, with strongly demethylated heterochromatin and methylated euchromatin. This suggests that chromosome DNA methylat… Show more

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Cited by 14 publications
(20 citation statements)
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“…In both cell types staining is not detected at pericentromeric heterochromatic regions as shown after counterstaining with DAPI (blue, arrows in B and C). (C) In 8-day-old mice spermatogonia metaphase chromosomes (with premature centromeric region separation as previously described) (Bernardino-Sgherri et al, 2002) exhibit the same staining pattern. At different stages of adult germ cell, chromatin of pachytene (D), metaphase I (E) and metaphase II (F) spermatocytes is stained by anti-KIN17 antibodies, whereas reduced staining is observed at pericentromeric regions (arrows in E and F).…”
Section: Kin17 Protein Detection In Adult Mouse Testicular Sections Amentioning
confidence: 56%
“…In both cell types staining is not detected at pericentromeric heterochromatic regions as shown after counterstaining with DAPI (blue, arrows in B and C). (C) In 8-day-old mice spermatogonia metaphase chromosomes (with premature centromeric region separation as previously described) (Bernardino-Sgherri et al, 2002) exhibit the same staining pattern. At different stages of adult germ cell, chromatin of pachytene (D), metaphase I (E) and metaphase II (F) spermatocytes is stained by anti-KIN17 antibodies, whereas reduced staining is observed at pericentromeric regions (arrows in E and F).…”
Section: Kin17 Protein Detection In Adult Mouse Testicular Sections Amentioning
confidence: 56%
“…After birth, we found that the strong overall DNA methylation of euchromatin decreased along with successive divisions corresponding to a passive mechanism of demethylation (Coffigny et al, 1999;Bernardino et al, 2000;Bernardino-Sgherri et al, 2002a). Thus, the methylation status of euchromatin and heterochromatin was completely different.…”
Section: Dna Methylation Changes During Spermatogenesismentioning
confidence: 73%
“…Thus, the methylation status of euchromatin and heterochromatin was completely different. Whereas euchromatin was strongly methylated in germ cells just leaving their cycle arrest, juxtacentromeric heterochromatin remained weakly methylated (Coffigny et al, 1999;Bernardino-Sgherri et al, 2002a). With successive divisions, whereas euchromatin was losing its strong DNA methylation, juxtacentromeric heterochromatin was progressively methylated, involving chromosomes at random (unpublished data).…”
Section: Dna Methylation Changes During Spermatogenesismentioning
confidence: 99%
“…The labeling of heterochromatin is much more variable from species to species, being very intense in M. musculus, mild in R. norvegicus, and not detectable in E. fulvus. Differences also exist between germ cells and somatic testicular cells in both the mouse and rat during development (Bernardino-Sgherri and Dutrillaux, 2001;Bernardino-Sgherri et al, 2002).…”
Section: Dna Methylation and Chromosome Banding In Autosomesmentioning
confidence: 99%
“…Accessibility to the target was achieved by either UV exposure (Miller et al, 1982) or successive pepsin and HCl treatments (Barbin et al, 1994) of metaphase chromosome spreads. The original protocols were slightly modified according to the species and tissue for chemical treatment (Bernardino et al, 1996Vilain et al, 2000;Bernardino-Sgherri and Dutrillaux, 2001;Bernardino-Sgherri et al, 2002); in each case, however, the cells were exposed to UV light for 12 h.…”
Section: Immunostaining With Anti-5mc Antibodymentioning
confidence: 99%