Unusual metabolism of 5 S RNA in HeLa cells

Leibowitz, Robert D.; Weinberg, Robert A.; Penman, Sheldon

doi:10.1016/0022-2836(73)90166-6

Cited by 47 publications

(26 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If so, then the regulatory molecule may be present in normal amounts in o/o and p 1-11o embryos which would account for the normal transcription of 5S RNA in these mutants . The synthesis of normal amounts of 5S RNA in o/o and pt -I/o embryos and the finding that in HeLa cells newly synthesized 5S RNA is found initially in a soluble form in the cytoplasm (20) are also consistent with the idea, originally suggested by Knight and Darnell (17), that 5S RNA has a function other than its role in ribosomes .…”

supporting

confidence: 88%

“…Perry and Kelley (28) (16,20,34) and ovaries of Xenopus (12,22) [11]) . The partial nucleolate embryos (p t -t/o) were obtained from a cross between a female +/o frog and a male +/pi -t frog.…”

Section: Introductionmentioning

confidence: 99%

“…A substantial nuclear pool of 5S RNA exists (17) and 5S RNA is synthesized during mitosis whereas 18S and 28S RNA synthesis is repressed (34) . More recently it has been shown that about four times as much 5S RNA is made in exponentially growing HeLa cells than is required for the assembly of new ribosomes (20) . This latter observation and the clear noncoordinate synthesis of the three rRNAs during oogenesis of Xenopus (12,22) represent the best evidence that in normal situations equal numbers of 5S, 18S, and 28S RNA are not synthesized .…”

mentioning

confidence: 99%

See 2 more Smart Citations

CONTROL OF 5S RNA SYNTHESIS DURING EARLY DEVELOPMENT OF ANUCLEOLATE AND PARTIAL NUCLEOLATE MUTANTS OF XENOPUS LAEVIS

Miller

1973

The Journal of Cell Biology

View full text Add to dashboard Cite

Ribosomes of all eukaryotes contain a single molecule of 5S, 18S, and 28S RNA. In the frog Xenopus laevis the genes which code for 18S and 28S RNA are located in the nucleolar organizer, but these genes are not linked to the 5S RNA genes. Therefore the synthesis of the three ribosomal RNAs provides a model system for studying interchromosomal aspects of gene regulation. In order to determine if the synthesis of the three ribosomal RNAs are interdependent, the relative rate of 5S RNA synthesis was measured in anucleolate mutants (o/o), which do not synthesize any 18S or 28S RNA, and in partial nucleolate mutants (pl-1/o), which synthesize 18S and 28S RNA at 25% of the normal rate. Since the o/o and pl-1/o mutants have a complete and partial deletion of 18S and 28S RNA genes respectively, but the normal number of 5S RNA genes, they provide a unique system in which to study the dependence of 5S RNA synthesis on the synthesis of 18S and 28S RNA. Total RNA was extracted from embryos labeled during different stages of development and analyzed by polyacrylamide gel electrophoresis. Quite unexpectedly it was found that 5S RNA synthesis in o/o and pl-1/o mutants proceeds at the same rate as it does in normal embryos. Furthermore, 5S RNA synthesis is initiated normally at gastrulation in o/o mutants in the complete absence of 18S and 28S RNA synthesis.

show abstract

supporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

CONTROL OF 5S RNA SYNTHESIS DURING EARLY DEVELOPMENT OF ANUCLEOLATE AND PARTIAL NUCLEOLATE MUTANTS OF XENOPUS LAEVIS

Miller

1973

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…[3H]uridine were reported by Leibowitz et al [4]. They suggested that this is due to the rapid release of newly synthesized nuclear SSRNA into the cytosolic fraction.…”

Section: Discussionmentioning

confidence: 91%

“…Leibowitz et al (1973) [4] presented a kinetic study on radiolabeling of SSRNA from various cellular components in HeLa cells with [3H]uridine; this study is relevant to our investigations. The authors indicated that newly synthesized SSRNA appeared rapidly in the cytosolic fraction.…”

mentioning

confidence: 99%

Metabolic characteristics of rat liver cytosolic 5SRNA

Ogata

Kurahashi

Nishiyama

et al. 1993

European Journal of Biochemistry

View full text Add to dashboard Cite

Institute for Gene Expression, Dobashi Kyoritsu Hospital, Matsuyama, Japan SSRNA were purified from the cytosol, ribosomes, nucleoli and nucleoplasm of rat liver. The 5'-terminal and 3'-terminal sequences of these SSRNA species were shown to be the same. The time course of changes in specific activities of these SSRNA in rat liver, 20 min to 16 h after intraperitoneal injection of ['4C]orotic acid, were investigated. The specific activity of cytosolic SSRNA increased almost linearly for 3 h, without a lag, to a high value, then decreased rapidly. The specific activity of cytosolic SSRNA was much higher than the specific activities of SSRNA from the other cellular components over 45 min to 6 h after intraperitoneal injection, although at 20 min the specific activity was similar to that of nucleoplasmic SSRNA. Thus, cytosolic SSRNA of rat liver is characterized by a high rate of turnover, which was shown to be comparable to that of heterogeneous nuclear RNA. The specific activity of SSRNA, 45 min to 6 h after intraperitoneal injection, was as follows ; cytosolic > nucleoplasmic > nucleolar > ribosomal SSRNA. The specific activity of nucleoplasmic RNA that migrated slightly slower than nucleoplasmic SSRNA on PAGE was much higher than that of cytosolic SSRNA or nucleoplasmic SSRNA, and so may be the precursor of nucleoplasmic SSRNA. Together, these results indicate that cytosolic SSRNA is transferred from the nuclei to the cytosol immediately after maturation of the precursor of nucleoplasmic SSRNA without entering the nucleoplasmic pool of SSRNA.Previously, we reported [l] that most SSRNA in rat liver cytosol is present in two high molecular-mass fractions that can be separated by Sephadex G200 column chromatography. One of these fractions, eluted in the void volume, contained nine aminoacyl-tRNA synthetases. This macromolecular aminoacyl-tRNA synthetase complex was purified and found to contain SSRNA and the ribosomal protein L5, probably as a SSRNA-LS complex (SSRNP). Furthermore, SSRNA and SSRNP were found to function in the complex as positive effectors of methionyl-tRNA and isoleucyl-tRNA synthetases [2].Recently, we purified a fraction containing threonyltRNA and histidyl-tRNA synthetases from a second high molecular-mass fraction (fraction 11) obtained by chromatography on Sephadex G200, and found that this fraction also contained SSRNA and protein L5 (unpublished results).There are only a few reports on the metabolism of cytosolic SSRNA in mammalian cells; Zehabi-Willner and Danon [3] reported that the rate of radiolabeling of cytosolic SSRNA was much lower than that of ribosomal SSRNA in rabbit reticulocytes. Leibowitz et al. (1973) [4] presented a kinetic study on radiolabeling of SSRNA from various cellular components in HeLa cells with [3H]uridine; this study is relevant to our investigations. The authors indicated that newly synthesized SSRNA appeared rapidly in the cytosolic fraction. It then associated with the nucleolus via the nucleoplasm, where it was finally incorporated into large ribosomal subu...

show abstract

RNA Polymerase III and Transcription of 5S Ribosomal DNA

Fürth¹

1989

Molecular Biology of Chromosome Function

View full text Add to dashboard Cite

Unusual metabolism of 5 S RNA in HeLa cells

Cited by 47 publications

References 16 publications

CONTROL OF 5S RNA SYNTHESIS DURING EARLY DEVELOPMENT OF ANUCLEOLATE AND PARTIAL NUCLEOLATE MUTANTS OF XENOPUS LAEVIS

CONTROL OF 5S RNA SYNTHESIS DURING EARLY DEVELOPMENT OF ANUCLEOLATE AND PARTIAL NUCLEOLATE MUTANTS OF XENOPUS LAEVIS

Metabolic characteristics of rat liver cytosolic 5SRNA

RNA Polymerase III and Transcription of 5S Ribosomal DNA

Contact Info

Product

Resources

About