Unusual microbial community and impact of iron and sulfate on microbial fuel cell ecology and performance

Mancílio, Lucca Bonjy Kikuti; Ribeiro, Guilherme Augusto; Lopes, Érica Mendes; Kishi, Luciano Takeshi; Martins-Santana, Leonardo; Siqueira, Guilherme Marcelino Viana de; Andrade, André; Guazzaroni, María-Eugenia; Reginatto, Valéria

doi:10.1016/j.crbiot.2020.04.001

Cited by 28 publications

(4 citation statements)

References 67 publications

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“…Due to the production of some natural mediator molecules and the metabolic ability of this microorganism to decompose pollutant oxidizable substrates and recover energy, Pseudomonas sp. are promising biocatalysts, being reported as one of the most representative genera in electrogenic biofilm consortia in MFCs in medium with acetate [ 35 , 36 ] or glycerol [ 37 ]. In addition to these natural characteristics, Pseudomonas can be easily modified with several synthetic engineering techniques and tools that can be useful to increase productivity in MFCs, such as increasing power density and current density [ 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

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Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC’s anode. Moreover, Pseudomonas species can grow under low-oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species grown with 1 g.L−1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m−2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium’s potential as a synthetic biology non-model chassis. Through testing various genetic parts, including constitutive promoters, replication origins and cargos using pSEVA vectors as a scaffold, we assessed the bacterium’s suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

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Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to the production of some natural mediator molecules and the metabolic ability of this microorganism to decompose pollutant oxidizable substrates and recover energy, Pseudomonas sp. are promising biocatalysts, being reported as one of the most representative genera in electrogenic biofilm consortia in MFCs in medium with acetate [35,36] or glycerol [37]. In addition to these natural characteristics, Pseudomonas can be easily modified with several synthetic engineering techniques and tools that can be useful to increase productivity in MFCs, such as increasing power density and current density [23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Preprint

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Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC's anode. Moreover, Pseudomonas species can grow under low oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species, grown with 1 g.L-1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m-2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium's potential as a synthetic biology non model chassis. Through testing various genetic parts, including constitutive promoters, replication origins, and cargos using pSEVA vectors as a scaffold, we assessed the bacterium's suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

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“…Moreover, each species in the anode biofilm play a synergetic effect in pollutant removal and energy generation (Guo et al 2020). However, according to Mancílio et al (2020) the microbes in the anode biofilms may have antagonistic effect that requires further study; however, increasing diverse anode biofilm communities can simultaneously improve energy generation and wastewater treatment (Abbassi et al 2020;Santoro et al 2017).…”

Section: Introductionmentioning

confidence: 99%

New fragmented electro-active biofilm (FAB) reactor to increase anode surface area and performance of microbial fuel cell

Atnafu

Leta

2021

Environ Syst Res

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Background Microbial fuel cell (MFC) technology is a promising sustainable future energy source with a renewable and abundant substrate. MFC critical drawbacks are anode surface area limitations and electrochemical loss. Recent studies recommend thick anode biofilm growth due to the synergetic effect between microbial communities. Engineering the anode surface area is the prospect of MFC. In this study, a microbial electrode jacket dish (MEJ-dish) was invented, first time to the authors’ knowledge, to support MFC anode biofilm growth. The MFC reactor with MEJ-dish was hypothesized to develop a variable biofilm thickens. This reactor is called a fragmented electro-active biofilm-microbial fuel cell (FAB-MFC). It was optimized for pH and MEJ-dish types and tested at a bench-scale. Results Fragmented (thick and thin) anode biofilms were observed in FAB-MFC but not in MFC. During the first five days and pH 7.5, maximum voltage (0.87 V) was recorded in MFC than FAB-MFC; however, when the age of the reactor increases, all the FAB-MFC gains momentum. It depends on the MEJ-dish type that determines the junction nature between the anode and MEJ-dish. At alkaline pH 8.5, the FAB-MFC generates a lower voltage relative to MFC. On the contrary, the COD removal was improved regardless of pH variation (6.5–8.5) and MEJ-dish type. The bench-scale studies support the optimization findings. Overall, the FAB improves the Coulombic efficiency by 7.4–9.6 % relative to MFC. It might be recommendable to use both FAB and non-FAB in a single MFC reactor to address the contradictory effect of increasing COD removal associated with the lower voltage at higher pH. Conclusions This study showed the overall voltage generated was significantly higher in FAB-MFC than MFC within limited pH (6.5–7.5); relatively, COD removal was enhanced within a broader pH range (6.5–8.5). It supports the conclusion that FAB anode biofilms were vital for COD removal, and there might be a mutualism even though not participated in voltage generation. FAB could provide a new flexible technique to manage the anode surface area and biofilm thickness by adjusting the MEJ-dish size. Future studies may need to consider the number, size, and conductor MEJ-dish per electrode.

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Unusual microbial community and impact of iron and sulfate on microbial fuel cell ecology and performance

Cited by 28 publications

References 67 publications

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

New fragmented electro-active biofilm (FAB) reactor to increase anode surface area and performance of microbial fuel cell

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