“…The HAI titer was determined by visual inspection as the highest dilution that inhibited erythrocyte agglutination, using standard criteria. MN titers were measured as previously described (38). Briefly, confluent monolayers of Madin-Darby canine kidney (MDCK) cells (British Colombia Center for Disease Control) were incubated in 96-well flat-bottom plates (Falcon Corning Life Science, Corning, NY) in MegaVir (VWR, Radnor, PA) supplemented with 10 g/ml gentamicin (Gibco Life Technologies, Burlington, ON, Canada), 0.25 g/ml amphotericin B (Gibco Life Technologies, Burlington, ON, Canada), 100,000 U/ml penicillin G (Sigma, St. Louis, MO), and 10 g/ml glutamine (Wisent, St. Bruno, QC, Canada).…”