Unusual Properties of Hyaluronateprotein Isolated From Pathological Synovial Fluids*

Hamerman, David; Sandson, John

doi:10.1172/jci104873

Cited by 35 publications

(17 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rate of loss of '4Mn from the liver became significantly accelerated. This was in accord with observations on patient 9 (see text) and operated to diminish the differences between arthritics and controls. cally thereafter.…”

Section: Methodssupporting

confidence: 92%

“…Such a block would explain some of the abnormalities of mucopolysaccharide metabolism in rheumatoid arthritis (7)(8)(9)(10). Of these, the one discovered by Laskin, Engel, Joseph, and Pollak (8) is intriguing because it proved reversible by the administration of glucocorticoid hormones.…”

Section: Discussionmentioning

confidence: 99%

“…The postulate that abnormal manganese metabolism might be present in human collagen disease rests on the following: (a) Rheumatoid arthritis displays abnormalities of mucoprotein and mucopolysaccharide metabolism (7)(8)(9)(10); (b) Another collagen disease, the hydralazine syndrome, appeared linked to manganese deficiency on the basis of animal studies (11,12) ; (c) Induction of the hydralazine syndrome seems to depend upon the genetic substratum (13); and (d) Genetic factors may interact with environmental ones in the development of manganese deficiency, as was proven by the manganese-dependent defects of the pallid strain of mice (14,15).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Slow turnover of manganese in active rheumatoid arthritis accelerated by prednisone

Cotzias¹,

Papavasiliou²,

Hughes³

et al. 1968

J. Clin. Invest.

View full text Add to dashboard Cite

Total body and area counts of intravenously injected 54Mn were measured periodically in 29 in-patients. A heterogeneous group of 19 control patients showed fair reproducibility in the immediate distribution, and considerable individual variance in the subsequent loss of the isotope. Eight studies of the effects of feeding excesses of manganous sulfate to five patients showed acceleration of the rate of loss of the radioisotope from the whole body and the liver. These findings seem compatible with the presence of control mechanisms in man, operating to vary the metal's excretion, while tending to preserve constancy of its concentration in tissues.Slow turnover rates of the metal were demonstrated in seven out of eight patients with active rheumatoid arthritis, in one with hydralazine disease, but not in one arthritic undergoing an impressive, spontaneous remission. Statistically significant differences were encountered in the measurements of 54Mn turnover of the total body, the thyroid, and the liver.Administration of prednisone induced clinical improvement and significant acceleration of these turnovers. Slow turnovers are characteristic of nutritional manganese deficiency. Therefore, serum and blood manganese determinations were performed by neutron activation analysis on 14 control patients, and on six patients with active rheumatoid arthritis. A statistically significant elevation of the red cell manganese concentration was encountered in patients with rheumatoid arthritis. This argued against the presence of classical trace-

show abstract

Section: Methodssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Slow turnover of manganese in active rheumatoid arthritis accelerated by prednisone

Cotzias¹,

Papavasiliou²,

Hughes³

et al. 1968

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The extremely high viscosity values observed with 5 of the knee fluid samples were probably due to interactions between the hyaluronic acid and other synovial fluid components (18).…”

Section: Resultsmentioning

confidence: 90%

The lubricating activity of human synovial fluids

Swann¹,

Bloch²,

Swindell³

et al. 1984

Arthritis & Rheumatism

View full text Add to dashboard Cite

The lubricating abilities of human synovial fluids were measured using a rotating cartilage-on-glass apparatus. A total of 247 human fluids were lubricationtested. Of these, 20 of the 180 knee fluids from patients with degenerativehaumatic joint disease lubricated less well than normal bovine synovial fluid. The remainder of the fluids from the knee and other joints were equivalent to normal bovine synovial fluid in their lubricating properties. The concentrations of hyaluronic acid, protein, and sialic acid and the relative viscosity of 117 human fluid samples were determined, but no relationships between the gross composition and the lubricating abilities were apparent.Several types of friction measuring devices have been used to measure the lubricating ability of synovial fluids, and a number of mechanisms by which synovial fluid lubricates articular cartilage have been suggested (1-3). Possibly a combination of mechanisms, working in concert, enables synovial fluid to function as a cartilage lubricant. The extent to which failure of one or more of these mechanisms may limit the lubricating functions of synovial fluid, and contribute to the occurrence of joint diseases, is not known.Our approach to the study of cartilage lubrication has been to fractionate synovial fluid by biochemical procedures and to determine which components are able to act as lubricants in a friction measuring device. In initial studies (4,5), an oscillating cartilageon-cartilage test system was used, and we were able to determine that a purified synovial fluid glycoprotein fraction was able to lubricate articular cartilage. More recently, a simplified rotating cartilage-on-glass friction measuring apparatus was used ( 6 ) . Solutions containing a single detectable glycoprotein (lubricin) (7) at concentrations as low as 30 pgiml had lubricating properties that were similar, if not identical, to those of whole synovial fluid. These results showed that lubricin was an extremely efficient lubricant for articular cartilage and indicated that lubricin was the component that endows synovial fluid with its boundary lubricating properties (8,9).The prior studies were all performed on bovine metacarpophalangeal synovial fluids. The present experiments were undertaken to obtain information about the lubricating ability of human synovial fluids by performing tests on pathologic samples using the rotating cartilage-on-glass test system. PATIENTS AND METHODSSynovial fluids. Human synovial fluids were obtained from patients seen at the Arthritis Unit at the Massachusetts General Hospital. Following the completion of clinical laboratory tests, the samples were stored at 4°C for variable lengths of time (1-4 days). The samples were clarified by

show abstract

“…The hyaluronateprotein isolated from normal synovial fluid differs from hyaluronateprotein isolated from many different types of pathologic synovial fluid (1,2). Normal hyaluronateprotein (NHP) contains more protein, and exhibits physical and immunologic properties different from pathologic hyaluronateprotein (PHP).…”

mentioning

confidence: 99%

The source of the inter‐alpha trypsin inhibitor in pathologic hyaluronateprotein

Becker¹,

Sandson²

1971

Arthritis & Rheumatism

Self Cite

View full text Add to dashboard Cite

Hyaluronateprotein isolated from pathologic synovial fluid has been shown to have different physical properties from hyaluronateprotein isolated from normal synovial fluid. The altered properties of hyaluronateprotein from pathologic synovial fluid are due to the presence of a serum protein identified immunologically as the inter‐alpha trypsin inhibitor (IATI). The IATI is undetectable in normal synovial fluid, but is present in pathologic synovial fluids. The IATI in pathologic synovial fluid is bound to hyaluronate. The concentration of IATI is always much higher in serum than synovial fluid indicating that the IATI in synovial fluid has passed from the serum into the diseased joint where it has become firmly bound to hyaluronate.

show abstract

Unusual Properties of Hyaluronateprotein Isolated From Pathological Synovial Fluids*

Cited by 35 publications

References 5 publications

Slow turnover of manganese in active rheumatoid arthritis accelerated by prednisone

Slow turnover of manganese in active rheumatoid arthritis accelerated by prednisone

The lubricating activity of human synovial fluids

The source of the inter‐alpha trypsin inhibitor in pathologic hyaluronateprotein

Contact Info

Product

Resources

About