Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba

Pfeiffer, P.; Jung, Jean-Luc; Heitzler, Jacques; Keith, Gérard

doi:10.1099/0022-1317-74-6-1167

Cited by 27 publications

(14 citation statements)

References 33 publications

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“…The 16.7-kb dsRNA found in V. faba contains the single long ORF and the site-specific nick (28). Furthermore, the deduced amino acid sequence of the ORF is similar to those of the ORFs in rice dsRNAs (29).…”

Section: Two Endogenous Dsrnas In Ricecultivated (O Sativa) and Wildmentioning

confidence: 82%

Molecular Characterization of Two Endogenous Double-stranded RNAs in Rice and Their Inheritance by Interspecific Hybrids

Moriyama

Horiuchi

Koga

et al. 1999

Journal of Biological Chemistry

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We completely sequenced 13,936 nucleotides (nt) of a double-stranded RNA (dsRNA) of wild rice (W-dsRNA). A single long open reading frame (13,719 nt) containing the conserved motifs of RNA-dependent RNA polymerase and RNA helicase was located in the coding strand. The identity between entire nucleotide sequence of WdsRNA and that of the dsRNA of temperate japonica rice (J-dsRNA, 13,952 nt) was 75.5%. A site-specific discontinuity (nick) was identified at nt 1,197 from the 5 end of the coding strand of W-dsRNA. This nick is also located at nt 1,211 from the 5 end in the coding strand of JdsRNA. The dsRNA copy number was increased more than 10-fold in pollen grains of both rice plants. This remarkable increase may be responsible for the highly efficient transmission of J-dsRNA via pollen that we already reported. J-dsRNA and W-dsRNA were also efficiently transmitted to interspecific F1 hybrids. Seedmediated dsRNA transmission to F2 plants was also highly efficient when the maternal parent was wild rice. The efficiency of dsRNA transmission to F2 plants was reduced when the maternal parent was temperate japonica rice; however, the reduced rates in F2 plants were returned to high levels in F3 plants.Linear large (more than 10 kb 1 ) double-stranded RNAs (dsRNAs) have frequently been identified in healthy plants such as alfalfa (1), barley (2, 3), broad bean (Vicia faba (4, 5)), cassava (6), common bean (Phaseolus vulgaris (7, 8)), pepper (9, 10) and rice (11-13). Most of these dsRNAs have no obvious effect on the phenotype of the host plant, with the exception of the dsRNA in V. faba, that is associated with cytoplasmic male sterility (4,14). These large dsRNAs are not associated with distinct virus-like particles (2, 13). They are present at a (low) constant concentration in host plants (15) and replicate using their own RNA-dependent RNA polymerase (5). Although they are usually found in the cytoplasm of host plant cells, they are effectively transmitted to progeny plants via pollen as much as by ova (2, 15). Because all attempts (mechanical inoculation, graft transmission, and aphid transmission) other than by seeds failed to transmit dsRNA to dsRNA-free plants, dsRNA is probably transmitted to progeny plants via seeds alone (2, 9, 15). Thus, these endogenous dsRNAs have some intriguing plasmid-like properties that differ from those of conventional plant RNA viruses (16).Double-stranded RNA (about 14 kb) is a feature of many strains of temperate and tropical japonica rice (cultivated rice, Oryza sativa) and of one strain of wild rice (Oryza rufipogon W-1714, an ancestor of O. sativa). It is not found in any strains of indica rice (cultivated rice, O. sativa), which rarely hybridizes with japonica rice in the field (13). These dsRNAs occur in every tissue as well as at every developmental stage, and they are transmitted very efficiently (more than 98%) to progeny plants via seeds (15). The dsRNAs are maintained at an almost constant concentration (100 copies/cell) by host plants from generation to generation (15)....

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Section: Two Endogenous Dsrnas In Ricecultivated (O Sativa) and Wildmentioning

confidence: 82%

Molecular Characterization of Two Endogenous Double-stranded RNAs in Rice and Their Inheritance by Interspecific Hybrids

Moriyama

Horiuchi

Koga

et al. 1999

Journal of Biological Chemistry

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“…Other highly similar regions were found among assigned members of the genus Endornavirus [Vicia faba endornavirus (VFV; Pfeiffer et al, 1993), Oryza sativa endornavirus (OSV; Fukuhara et al, 1995) and Oryza rufipogon endornavirus (ORV; Moriyama et al, 1999)], two unclassified members of Phytophthora endornavirus 1 (PEV1; Hacker et al, 2005) as well as Helicobasidium mompa endornavirus (HmEV1-670; Osaki et al, 2006). The expect value of these viruses ranged from 2E-72 to 3E-19.…”

mentioning

confidence: 99%

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Tuomivirta¹,

Kaitera²,

Hantula³

2009

Journal of General Virology

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A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities Ascospore and mycelial isolates of Gremmeniella abietina type B were found to contain three different dsRNA molecules with approximate lengths of 11, 5 and 3 kb. The 11 kb dsRNA encoded the genome of a putative virus and is named Gremmeniella abietina type B RNA virus XL (GaBRV-XL). GaBRV-XL probably exists in an unencapsulated state. We identified two distinct dsRNAs (10 374 and 10 375 bp) of GaBRV-XL, both of which coded for the same putative polyprotein (3249 amino acids) and contained four regions similar to putative viral methyltransferases, DExH box helicases, viral RNA helicase 1 and RNA-dependent RNA polymerases. While a cysteine-rich region with several CxCC motifs in GaBRV-XL was similar to that of putative endornaviruses, cluster analyses of conserved regions revealed GaBRV-XL to be distinct from a broad range of viral taxa but most closely related to Discula destructiva virus 3. Collectively, these findings suggest that GaBRV-XL represents a novel virus group related to endornaviruses. INTRODUCTIONRecently recognized by the International Committee on Taxonomy of Viruses (ICTVdB Management, 2006), endornaviruses are usually cryptic and non-enveloped plant and fungal dsRNA viruses that spread efficiently through mitotic and meiotic cells but not through grafts (Fukuhara et al., 1995;Moriyama et al., 1999;Wakarchuk & Hamilton, 1990;Pfeiffer, 1998 Fungal viruses of the G. abietina species complex have been studied thoroughly in type A and are known to include putative members of the virus families Narnaviridae, Totiviridae and Partitiviridae, some of which can co-infect a single fungal isolate (Tuomivirta et al., 2002;Tuomivirta & Hantula, 2005).The goals of this study include a survey of dsRNA molecules in G. abietina type B, molecular characterization of the most common dsRNA type and its comparison to representatives of similar viral taxa. METHODSG. abietina type B strains. G. abietina type B strains (see Supplementary Table S1, available in JGV Online) were identified by the random amplified microsatellite (RAMS) technique of Hantula & Müller (1997). Branches from P. sylvestris and Pinus contorta ,2 m in height (,20 years old) with symptoms of scleroderris canker were collected in artificially or naturally regenerated stands between June 1994 and June 1995 in northern Finland. Disease symptoms included death of lateral branches over several internodes, cankers, yellowgreen woody tissues, pycnidia and apothecia. Fungi were isolated either from pycnidia or from infected wood adjacent to apothecia.Nucleic acid isolation and electrophoresis. G. abietina type B isolates were grown at 20 uC on modified orange serum agar covered with a cellophane membrane (Müller et al., 1994). dsRNA isolationThe GenBank/EMBL/DDBJ accession numbers for the complete sequences of the 11 kb dsRNAs of isolates AU58 (GaBRV-XL1) and E46 (GaBRV-XL2) are respectively DQ399289 and DQ399290.Details of ...

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“…The system CMS447 is very peculiar, since a genetic restoration of pollen fertility becomes permanent; offspring that segregates from a heterozygously restored plant does not segregate for the pollen sterility as expected from textbook schemes on cytoplasmic male sterility (CMS) systems. Molecular genetic findings proposed that CMS447 may be the result of an infection with a defect virus, and that restoration corresponds to nontransmittance by seeds (Pfeiffer et al, 1993). In Germany, Link et al (1997) detected two other CMS systems (CMS199, CMS297), yet due to instability of the sterility, especially due to spontaneous reversions to pollen fertility, hybrid breeding still could not be realized in faba bean.…”

Section: Traditional Breedingmentioning

confidence: 99%

Faba Bean

Link

Hanafy

Malenica

et al. 2008

Compendium of Transgenic Crop Plants

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Vicia faba L. is an old world grain legume, combine harvested for food and feed in Europe, North and East Africa, Asia, and in the northwest of South America. It is an annual, diploid plant ( x = 6), with a large genome (1C ∼ 13 pg of DNA), large seeds (0.3 up to >2 g per seed), and a protein content of about 30%. Being a small agricultural species, a relatively large producer in Europe is the United Kingdom with more than 150000 ha. On the global scale, China is the largest producer (39% of global area of 2.6 million hectares). Important fungal diseases are Botrytis fabae , Ascochyta fabae , and Uromyces viciae‐fabae . Important pests are Aphis fabae and Bruchus rufimanus . Broomrape ( Orobanche crenata ) is an important parasitic weed in the Mediterranean basin and the Nile valley. Traditional breeding is hampered by the partial allogamous, entomophilous reproduction, with degrees of outcrossing between 20% and 75%, depending on the actual genotype and the environment. The faba bean cannot be crossed with related species, and it is known as recalcitrant crop as to in vitro technologies. Agrobacterium ‐mediated transformation of Vicia faba resulted in regeneration of first transgenic plants carrying the aspartate kinse III from E. coli and the 2S‐albumin 8 from sunflower. In addition to the optimization of the regeneration protocol, knowledge gained from the model legumes Medicago truncatula and Lotus japonicus should enable further progress in Vicia faba breeding.

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Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba

Cited by 27 publications

References 33 publications

Molecular Characterization of Two Endogenous Double-stranded RNAs in Rice and Their Inheritance by Interspecific Hybrids

Molecular Characterization of Two Endogenous Double-stranded RNAs in Rice and Their Inheritance by Interspecific Hybrids

A novel putative virus of Gremmeniella abietina type B (Ascomycota: Helotiaceae) has a composite genome with endornavirus affinities

Faba Bean

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