Unveiling metabolic changes in marsupialized odontogenic keratocyst: A pilot study

Abstract

Objective: We aimed to assess which metabolic pathways would be implicated in the phenotypic changes of the epithelial lining of odontogenic keratocyst after marsupialization, comparing pre-and post-marsupialized lesions with adjacent oral mucosa. Materials and Methods:Eighteen formalin-fixed and paraffin-embedded tissues from six subjects were divided into three paired groups: odontogenic keratocyst pre-(n = 6) and post-marsupialization (n = 6), and adjacent oral mucosa (n = 6). The metabolic pathways found in these groups were obtained by high-performance liquid chromatography-mass spectrometry-based untargeted metabolomics performed.Results: Through putative metabolite annotation followed by pathway enrichment and predictive analysis with automated algorithms (Mummichog and Gene Set Enrichment Analysis), we found differences in many cellular processes that may be involved in inflammation, oxidative stress response, keratinocyte-basal membrane attachment, differentiation, and proliferation functions, all relevant to odontogenic keratocyst pathobiology and the phenotype acquired after marsupialization. Conclusion:Our study was able to identify several metabolic pathways potentially involved in the metaplastic changes induced by marsupialization of odontogenic keratocysts. An improved comprehension of this process could pave the way for the development of targeted therapies.

Integrated proteomics, phosphoproteomics and metabolomics analyses reveal similarities among giant cell granulomas of the jaws with different genetic mutations

“…Sample preparation, data acquisition, and processing were performed according to the protocol described by Leite‐Lima et al 12 The specific parameters applied in XCMS version 3.8.2, which were optimized to our dataset using IPO version 1.12.0, are shown in Table S1.…”

L-cysteine contributes to destructive activities of odontogenic cysts/tumor

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Conservative treatment of mural unicystic ameloblastoma with posterior rehabilitation by implants: A dilemma in oral surgery

“…By contrast, some authors have discussed the clinical effectiveness of marsupialization/ decompression in the treatment of aggressive odontogenic lesions. 10,11 For instance, Consolo et al 10 through analyses of different histological sections of aggressive odontogenic keratocysts identified notorious changes in the cases treated with marsupialization, including cystic volume reduction due to the decrease in the proliferative activity of the odontogenic epithelium and, consequently, squamous cell differentiation and occurrence of fibrosis.…”