Unveiling structural and dynamical features of chromatin using NMR spectroscopy

Shi, Xiangyan

doi:10.1016/j.mrl.2024.200153

Magnetic Resonance Letters

2024

DOI: 10.1016/j.mrl.2024.200153

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Unveiling structural and dynamical features of chromatin using NMR spectroscopy

Xiangyan Shi

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Molecular Dynamics Simulations of Nucleosomes Containing Histone Variant H2A.J

Kosarim,

Fedulova,

Shariafetdinova

et al. 2024

IJMS

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Histone proteins form the building blocks of chromatin—nucleosomes. Incorporation of alternative histone variants instead of the major (canonical) histones into nucleosomes is a key mechanism enabling epigenetic regulation of genome functioning. In humans, H2A.J is a constitutively expressed histone variant whose accumulation is associated with cell senescence, inflammatory gene expression, and certain cancers. It is sequence-wise very similar to the canonical H2A histones, and its effects on the nucleosome structure and dynamics remain elusive. This study employed all-atom molecular dynamics simulations to reveal atomistic mechanisms of structural and dynamical effects conferred by the incorporation of H2A.J into nucleosomes. We showed that the H2A.J C-terminal tail and its phosphorylated form have unique dynamics and interaction patterns with the DNA, which should affect DNA unwrapping and the availability of nucleosomes for interactions with other chromatin effectors. The dynamics of the L1-loop and the hydrogen bonding patterns inside the histone octamer were shown to be sensitive to single amino acid substitutions, potentially explaining the higher thermal stability of H2A.J nucleosomes. Taken together, our study demonstrated unique dynamical features of H2A.J-containing nucleosomes, which contribute to further understanding of the molecular mechanisms employed by H2A.J in regulating genome functioning.

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Molecular Dynamics Simulations of Nucleosomes Containing Histone Variant H2A.J

Kosarim,

Fedulova,

Shariafetdinova

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

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Unveiling structural and dynamical features of chromatin using NMR spectroscopy

Cited by 1 publication

References 59 publications

Molecular Dynamics Simulations of Nucleosomes Containing Histone Variant H2A.J

Molecular Dynamics Simulations of Nucleosomes Containing Histone Variant H2A.J

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