Unveiling the antibacterial mechanism of resveratrol against Aeromonas hydrophila through proteomics analysis

Abstract

This investigation delves into elucidating the mechanism by which resveratrol (Res), a natural polyterpenoid renowned for its antimicrobial properties, exerts its effects on Aeromonas hydrophila, a ubiquitous waterborne pathogen. Our findings underscore the dose-dependent manifestation of resveratrol in exhibiting antibacterial and antibiofilm formation activities against A. hydrophila. Employing a Data-independent acquisition (DIA) based quantitative proteomics methodology, we systematically compared differentially expressed proteins in A. hydrophila subjected to varying concentrations of Res. Subsequent bioinformatics analyses revealed key proteins and pathways pivotal in resveratrol’s antimicrobial action, encompassing oxidative stress, energy metabolism, and cell membrane integrity. Validation of the proteomics outcomes was meticulously conducted using the qPCR method at the mRNA level. Dynamic trend analysis unveiled alterations in biological processes, notably the correlation between the cell division-related protein ZapC and resveratrol content. Furthermore, scanning electron microscopy corroborated a significant elongation of A. hydrophila cells, affirming resveratrol’s capability to inhibit cell division. In concert, resveratrol emerges as a participant in the cell membrane integrity pathway, biofilm formation, and potentially, the regulation of genes associated with cell division, resulting in morphological elongation. These revelations position resveratrol as a promising natural alternative to conventional antibiotics for treating A. hydrophila infections.

Mechanism of hypoxia-induced damage to the mechanical property in human erythrocytes—band 3 phosphorylation and sulfhydryl oxidation of membrane proteins

“…This suggests that excessive antioxidants may exhibit pro-oxidative (Giordano et al, 2020) and pro-membrane lipid peroxidation effects. Based on these findings, we chose 70 μM resveratrol to investigate the possible mechanism in the hypoxia model, which is also close to the concentration selected by other investigators in different disease models (Horn et al, 2007; Frontiers in Physiology frontiersin.org Saiko et al, 2008;Komorowska et al, 2023;Ei et al, 2024;Fu et al, 2024).…”

Natural Sunlight-Mediated Emodin Photoinactivation of Aeromonas hydrophila

“…Many traditional Chinese herbs have been developed for inhibiting A. hydrophila, because they are widely available and environmentally friendly. For example, resveratrol [22,23], thymol [24], fraxetin [25], oridonin [26], magnolol [27], baicalin [28], and emodin [29] from traditional Chinese herbs display an inhibitory activity against A. hydrophila. In addition, extracts from Ficus leaves [30], Moringa oleifera leaves [31], the Araucaria angustifolia [32] seed coat, and Hesperozygis ringens [33] also have the ability to inhibit the growth of A. hydrophila.…”

Enhancing the Bioavailability of Resveratrol: Combine It, Derivatize It, or Encapsulate It?

“…Additionally, hepatoma cell proliferation can be inhibited at 100-200 µM of RSV and hepatoma cell invasion suppression at 25 µM of RSV [62,63]. Additionally, RSV has been evaluated for its potential to inhibit the growth of some pathogens, such as Gram-positive and Gram-negative bacteria and fungi [74]. It has been found to be a potential beneficial agent for the treatment of Staphylococcus aureus pneumonia and other infections induced by S. aureus [75].…”