Unveiling the Differences of Secretome of Human Bone Marrow Mesenchymal Stem Cells, Adipose Tissue-Derived Stem Cells, and Human Umbilical Cord Perivascular Cells: A Proteomic Analysis

Abstract

The use of human mesenchymal stem cells (hMSCs) has emerged as a possible therapeutic strategy for CNS-related conditions. Research in the last decade strongly suggests that MSC-mediated benefits are closely related with their secretome. Studies published in recent years have shown that the secretome of hMSCs isolated from different tissue sources may present significant variation. With this in mind, the present work performed a comparative proteomic-based analysis through mass spectrometry on the secretome of hMSCs derived from bone marrow (BMSCs), adipose tissue (ASCs), and human umbilical cord perivascular cells (HUCPVCs). The results revealed that BMSCs, ASCs, and HUCPVCs differed in their secretion of neurotrophic, neurogenic, axon guidance, axon growth, and neurodifferentiative proteins, as well as proteins with neuroprotective actions against oxidative stress, apoptosis, and excitotoxicity, which have been shown to be involved in several CNS disorder/injury processes. Although important changes were observed within the secretome of the cell populations that were analyzed, all cell populations shared the capability of secreting important neuroregulatory molecules. The difference in their secretion pattern may indicate that their secretome is specific to a condition of the CNS. Nevertheless, the confirmation that the secretome of MSCs isolated from different tissue sources is rich in neuroregulatory molecules represents an important asset not only for the development of future neuroregenerative strategies but also for their use as a therapeutic option for human clinical trials.

Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF

“…MSCs secretomes present a high range of molecules, such as cytokines and growth factors 11, 30–32 . BDNF is a neurotrophin that, together with TrkB, its cognate receptor, is present throughout CNS 33 .…”

“…HUCPVC secretome present on its composition a wide range of molecules with different properties 31, 32 . Although our data shows a complete reversion of CM-induced axonal outgrowth when BDNF is depleted from the media, we cannot exclude the possibility that other factors present in HUCPVC secretome might also induce axonal outgrowth.…”

“…HUCPVCs were isolated as described previously 32 . HUCPVCs were ressuspended in α-Mem medium (Invitrogen, USA) supplemented with 1% of antibiotic/antimycotic (Invitrogen, USA) and 10% of FBS (Invitrogen, USA) and, subsequently, plated on culture at a density of 4.0 × 10 3 cells/cm 2 .…”

Influence of Mesenchymal Stem Cells Conditioned Media on Proliferation of Urinary Tract Cancer Cell Lines and Their Sensitivity to Ciprofloxacin

“…It was showed that stem cells derived from bone marrow, adipose tissue, and umbilical cord secrete 134 neuro‐regulatory molecules common to all three population. Still, 140 proteins were specific for stem cells isolated from fetal sources [Pires et al, ]. Pattern of protein expression was also modulated when culture conditions were changed from static to dynamic [Teixeira et al, ].…”

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

“…By profiling MSCs secretome characterization, it was found the presence of many cytokines, chemokines, Trubiani et al (2016) and growth factors through which MSCs promote neuronal survival increases process of neurogenesis and angiogenesis, modulate immune response, and inhibit neuronal cell death/apoptosis (Pires et al 2016). In addition to soluble factors, extracellular vesicles were found to be a key factors in cell-cell communication (Mittelbrunn and SanchezMadrid 2012).…”