Unveiling the Dual Role of the Dopaminergic System on Locomotion and the Innate Value for an Aversive Olfactory Stimulus in Drosophila

Fuenzalida‐Uribe, Nicolás; Campusano, Jorge M.

doi:10.1016/j.neuroscience.2017.12.032

Cited by 12 publications

(14 citation statements)

References 48 publications

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“…Actually, there seems to be a rather slow increase in the signal before an actual peak response is detected. This is consistent with the idea that Bz is a multisensorial stimulus, processed through different sensory systems (Fuenzalida‐Uribe & Campusano, 2018; Keene et al., 2004). In spite of this, the false color plot graph and PCR analysis show that the main neuroactive substance in the peak of the detected response is serotonin (Figures 5cii–iv,e).…”

Section: Resultssupporting

confidence: 89%

Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning

Hidalgo

Fuenzalida‐Uribe

Molina‐Mateo

et al. 2020

Journal of Neurochemistry

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A highly challenging question in neuroscience is to understand how aminergic neural circuits contribute to the planning and execution of behaviors, including the generation of olfactory memories. In this regard, electrophysiological techniques like Local Field Potential or imaging methods have been used to answer questions relevant to cell and circuit physiology in different animal models, such as the fly Drosophila melanogaster. However, these techniques do not provide information on the neurochemical identity of the circuits of interest. Different approaches including fast scan cyclic voltammetry, allow researchers to identify and quantify in a timely fashion the release of endogenous neuroactive molecules, but have been only used in in vitro Drosophila brain preparations. Here, we report a procedure to record for the first time the release of endogenous amines –dopamine, serotonin and octopamine‐ in adult fly brain in vivo, by fast scan cyclic voltammetry. As a proof of principle, we carried out recordings in the calyx region of the Mushroom Bodies, the brain area mainly associated to the generation of olfactory memories in flies. By using principal component regression in normalized training sets for in vivo recordings, we detect an increase in octopamine and serotonin levels in response to electric shock and olfactory cues respectively. This new approach allows the study of dynamic changes in amine neurotransmission that underlie complex behaviors in Drosophila and shed new light on the contribution of these amines to olfactory processing in this animal model.

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Section: Resultssupporting

confidence: 89%

Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning

Hidalgo

Fuenzalida‐Uribe

Molina‐Mateo

et al. 2020

Journal of Neurochemistry

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“…including courtship drive. [88][89][90][91][92] Serotonin is involved in many behaviors such as feeding, which directly affects longevity, 93 locomotion, 94 mating and courtship, 95 aggression, 96 and has modulatory effects on sleep-wake cycles, 97 and memory. 87,88 Some studies report decrease in dopaminergic cells viability with age, 78 while other do not.…”

Section: Neural Agingmentioning

confidence: 99%

“…88 However, the function of those dopaminergic cells, and the behaviors modulated by DA, might still be affected by aging. [88][89][90][91][92] Serotonin is involved in many behaviors such as feeding, which directly affects longevity, 93 locomotion, 94 mating and courtship, 95 aggression, 96 and has modulatory effects on sleep-wake cycles, 97 and memory. 98 Despite its wide-range involvement in behavior, and the fact that serotonin modulates the response to physical stress, 99 little attention has been given to studying the role of serotonin in aging.…”

Section: Neural Agingmentioning

confidence: 99%

Social behavior and aging: A fly model

Brenman-Suttner

Yost

Frame

et al. 2019

Genes Brain and Behavior

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The field of behavioral genetics has recently begun to explore the effect of age on social behaviors. Such studies are particularly important, as certain neuropsychiatric disorders with abnormal social interactions, like autism and schizophrenia, have been linked to older parents. Appropriate social interaction can also have a positive impact on longevity, and is associated with successful aging in humans. Currently, there are few genetic models for understanding the effect of aging on social behavior and its potential transgenerational inheritance. The fly is emerging as a powerful model for identifying the basic molecular mechanisms underlying neurological and neuropsychiatric disorders. In this review, we discuss these recent advancements, with a focus on how studies in Drosophila melanogaster have provided insight into the effect of aging on aspects of social behavior, including across generations.

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“…In D. melanogaster, there is one Vmat orthologue that encodes a protein responsible for packaging neurotransmitters such as dopamine, serotonin and octopamine into secretory vesicles [39,59]. Dopamine and serotonin are also vital for locomotion [60,61] and are also closely linked to stress and anxiety [55,62]. Therefore, it is possible and more likely that the locomotory phenotype that we observe were due to lowered packaging of monoamines.…”

Section: Discussionmentioning

confidence: 93%

The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster

Ceder

Lindberg

Perland

et al. 2021

Insects

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Cellular transport and function are dependent on substrate influx and efflux of various compounds. In humans, the largest superfamily of transporters is the SoLute Carriers (SLCs). Many transporters are orphans and little to nothing is known about their expression and/or function, yet they have been assigned to a cluster called atypical SLCs. One of these atypical SLCs is MFSD11. Here we present a first in-depth characterization of the MFSD11, CG18549. By gene expression and behavior analysis on ubiquitous and brain-specific knockdown flies. CG18549 knockdown flies were found to have altered adipokinetic hormone and adipokinteic hormone receptor expression as well as reduced vesicular monoamine transporter expression; to exhibit an altered locomotor behavior, and to have an altered reaction to stress stimuli. Furthermore, the gene expression of CG18549 in the brain was visualized and abundant expression in both the larvae and adult brain was observed, a result that is coherent with the FlyAtlas Anatomy microarray. The exact mechanism behind the observed behaviors is not fully understood, but this study provides new insights into the expression and function of CG18549. Clearly, these results provide a strong example as to why it is vital to fully characterize orphan transporters and through that gain knowledge about the body during normal condition and disease.

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Unveiling the Dual Role of the Dopaminergic System on Locomotion and the Innate Value for an Aversive Olfactory Stimulus in Drosophila

Cited by 12 publications

References 48 publications

Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning

Study of the release of endogenous amines in Drosophila brain in vivo in response to stimuli linked to aversive olfactory conditioning

Social behavior and aging: A fly model

The Fly Homologue of MFSD11 Is Possibly Linked to Nutrient Homeostasis and Has a Potential Role in Locomotion: A First Characterization of the Atypical Solute Carrier CG18549 in Drosophila Melanogaster

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