Unveiling the functional components and antivirulence activity of mustard leaves using an LC-MS/MS, molecular networking, and multivariate data analysis integrated approach

Ibrahim, Rana; Eltanany, Basma; Pont, Laura; Benavente, Fernando; El-Banna, S. A.; Otify, Asmaa M.

doi:10.1016/j.foodres.2023.112742

Cited by 20 publications

(7 citation statements)

References 36 publications

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“…Recently, chemometric methods have gained a great interest in many analytical techniques including chromatography [ 39 , 40 ], spectroscopy [ 41 , 42 ] and electrochemistry [ 43 , 44 ]. This approach fulfills the requirements of investigating a significant number of mixture components and enables to simultaneously evaluate a number of other variables.…”

Section: Introductionmentioning

confidence: 99%

A voltammetric method coupled with chemometrics for determination of a ternary antiparkinson mixture in its dosage form: greenness assessment

Hindam,

Eltanany,

Abou Al Alamein

et al. 2024

BMC Chemistry

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An electroanalytical methodology was developed by direct differential pulse voltammetric (DPV) measurement of Levodopa (LD), Carbidopa (CD) and Entacapone (ENT) mixture using bare glassy carbon electrode (GCE) in Britton Robinson (BR) buffer (pH = 2.0). A multivariate calibration model was then applied to the exported preprocessed voltammetric data using partial least square (PLS) as a chemometric tool. Additionally, the model was cross-validated and the number of latent variables (LVs) were determined to produce a reliable model for simultaneous quantitation of the three drugs either in their synthetic mixtures or in their marketed pharmaceutical formulation with high accuracy and precision. Data preprocessing was used to tackle the problem of lacking bi-linearity which is commonly found in electrochemical data. The proposed chemometric model was able to provide fast and reliable technique for quantitative determination of antiparkinson drugs in their dosage forms. This was successfully achieved by utilizing sixteen mixtures as calibration set and nine mixtures as validation set. The percent recoveries for LD, CD and ENT were found to be 100.05% ± 1.28%, 100.04% ± 0.53% and 99.99% ± 1.25%, respectively. The obtained results of the proposed method were statistically compared to those of a previously reported High Performance Liquid Chromatography (HPLC) methodology. Finally, the presented analytical method strongly supports green analytical chemistry regarding the minimization of potentially dangerous chemicals and solvents, as well as reducing energy utilization and waste generation.

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Section: Introductionmentioning

confidence: 99%

A voltammetric method coupled with chemometrics for determination of a ternary antiparkinson mixture in its dosage form: greenness assessment

Hindam,

Eltanany,

Abou Al Alamein

et al. 2024

BMC Chemistry

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“…integrifolia and var. rugosa, healthbeneficial compounds such as phenolics, glucosinolates and fatty acids were found to be highly accumulated in the chemical profiles (Ibrahim et al, 2023), making them promising as health-promoting vegetables.…”

Section: Introductionmentioning

confidence: 99%

“…Metabolomics, a potent analytical method, enables the comprehensive study of small molecules in biological systems (Hajjar et al, 2023), particularly healthbeneficial molecules. It has been used for untargeted metabolomics to elucidate metabolite alterations in various parts of plants, such as leaves (Ibrahim et al, 2023;Tunsagool et al, 2023). Metabolomics revealed the metabolite profile with altered levels of primary and secondary metabolites belonging to various classes, especially in green plants (Kumar et al, 2023a(Kumar et al, , 2023b linking to functional benefit prediction.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic profiling of health‐benefit compounds in fresh and preserved mustard greens

Tunsagool,

Pimpak,

Promwat

et al. 2023

Int J of Food Sci Tech

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SummaryIn today's consumer landscape, the health benefits of preserved foods, particularly pickled and salted mustard greens, are gaining recognition on a global scale. To compare health‐promoting compounds between fresh and preserved mustard greens, a comprehensive assessment using proximate composition analysis and untargeted metabolomics was conducted. The sub‐metabolome data revealed distinct profiles for each type of mustard greens, clearly seen in their separation through principal component analysis. The metabolite profiling identified 37 metabolites with significant differences (P‐value < 0.05, −log10 P‐value > 4). Fresh mustard greens were found to be rich in 16 identified metabolites, including sulfonic acids, purines, oxepanes, monosaccharides, fatty acids, amino acids and tripeptides, offering antioxidants, gut enhancers and immune boosters. In contrast, pickled mustard greens exhibited an abundance of 20 identified metabolites in the classes of flavonoids, amino acids and dipeptides, providing antioxidant properties. Salted mustard greens, on the other hand, primarily showcased a high level of uridine diphosphate glucose (a pyrimidine class metabolite), supporting glycoconjugate synthesis among all samples. This study highlights the prominent presence of various classes of metabolites associated with health‐benefit compounds in fresh and preserved mustard greens. These insights contribute to the evaluation of health benefits in the food industry when choosing between pickled and salted preservation methods.

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“…Combined with the characteristic fragment ions at m/z 116.9255 and m/z 150.9360 and further comparison with the reference compounds, it was identified as apigenin. Other flavonoids were identified by comparison of the accurate molecular weights and fragmentation patterns and related literature 18,[35][36][37][38].…”

mentioning

confidence: 99%

“…18,[35][36][37][38] Express components were accurately identified by comparison of the retention time, molecular weights, and MS/MS fragment data with those of the reference compounds; the others were putative by matching the accurate molecular weights and MS/MS fragment data and combining scores in the data processing software.…”

mentioning

confidence: 99%

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

Wang,

Zhang,

et al. 2024

Phytochemical Analysis

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IntroductionChangan powder (CAP) is mainly used to treat various intestinal diseases. Few studies on CAP have been reported; therefore, it is necessary to clarify the material basis of CAP to lay the foundation for further elucidating its functional mechanism and support the rational use of drugs.ObjectivesIn the present study, we aimed to propose a methodology for the quality control of CAP based on qualitative and quantitative analysis of its components.MethodsAn ultra‐performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UPLC‐FT‐ICR‐MS) method was developed to identify chemical components in CAP. In addition, fingerprints of 10 different batches of CAP were established, and quantitative analysis based on UPLC was performed to analyze the quality of CAP.ResultsA total of 58 compounds were preliminarily characterized. The similarity of 10 batches of CAP was greater than 0.995, and 23 common peaks were calibrated. Investigation of the quantitative analytical methodology showed that the four components had good linear relationships within their respective concentration ranges (r2 ≥ 0.9992), and the relative standard deviation (RSD) of precision and stability was less than 2%. The RSD of sample recovery ranged from 0.78% to 1.52%.ConclusionThe established method can quickly and effectively identify the chemical components of CAP and accurately quantify the known components in CAP. The established fingerprinting and content determination method is stable, reliable, and easy to operate and can be applied in quality control and in vivo research on CAP.

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Unveiling the functional components and antivirulence activity of mustard leaves using an LC-MS/MS, molecular networking, and multivariate data analysis integrated approach

Cited by 20 publications

References 36 publications

A voltammetric method coupled with chemometrics for determination of a ternary antiparkinson mixture in its dosage form: greenness assessment

A voltammetric method coupled with chemometrics for determination of a ternary antiparkinson mixture in its dosage form: greenness assessment

Metabolomic profiling of health‐benefit compounds in fresh and preserved mustard greens

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

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