Unveiling the intricacies of gene delivery: Caveolae-mediated endocytosis induces efficient mRNA delivery in slow-dividing cells

Therapeutic potential for mRNA-based IGF-I regenerative therapy

“… 8 When compared to other viral and DNA-based technologies, IVT mRNA therapeutics offer several advantages: it only requires cytoplasmic delivery, it utilizes endogenous translation mechanisms to transiently express proteins, and it poses low risks for genomic integration. 2 , 7 , 9 However, disadvantages of IVT mRNA therapeutics include mRNA instability, inefficient delivery, extracellular and intracellular barriers, and immunogenicity. 2 To address these limitations, many advances in IVT mRNA technologies utilize mRNA base modifications to minimize immune stimulatory effects, increase transcript stability, and augment translation.…”

“…should be evaluated in future studies, as unmodified mRNA-based delivery has been shown to be associated with immune responses. 2 , 9 Overall, Antony and colleagues provide an interesting investigation using novel SP-modified IGF-I mRNAs to augment translation, secretion, and localized persistence in targeted regions of muscle and spinal disc injury. The study further demonstrates that IGF-I mRNA expression remains localized to the targeted delivery site and also establishes optimized IGF-I mRNA dosages, which are important factors for achieving therapeutic efficacy and safety.…”