Job-related stress has been identified as the most challenging nursing issue, leading to emotional distress, burnout, and decreased job satisfaction. Nurses who exhibit resilience are better equipped to handle job-related stress and maintain their well-being, which positively impacts the quality of care provided to patients. This study aimed to determine the level of job-related stress, resilience, and well-being of staff nurses in a tertiary governmental hospital in Oman using a descriptive cross-sectional approach. A random sampling technique was used with a total of 145 nurses who completed a self-administered questionnaire consisting of the Perceived Stress Scale (PSS-10), the World Health Organization-Five Well-being Index (WHO-5), and the Brief Resilience Scale (BRS). Results of the study revealed that about 59% of nurses had a moderate perceived stress level, 39% had a low well-being level and 53.8 % had a low resilience level. Significant differences were found in perceived stress and resilience based on nurses' age and experience (p < 0.05). Omani nurses showed higher perceived stress levels (M = 25, SD = 6.93), lower resilience (M = 2.3, SD = 0.77), and lower well-being (M = 49.15, SD = 20.85) than nurses from other nationalities. Moreover, a moderate negative correlation was found between job-related stress and resilience (rs = -0.44, p < 0.01). These findings strongly suggest implementing educational training plans and intervention programs to reduce stress levels, build resilience, and improve the overall well-being of hospital nurses.