Up-regulation by overexpression of c-MET in fibroblastic foci of usual interstitial pneumonia

Melocchi, Laura; Cervi, Giulia; Sartori, Giuliana; Gandolfi, Laura; Jocollé, Genny; Cavazza, Alberto; Rossi, Giulio

doi:10.32074/1591-951x-920

Cited by 1 publication

(1 citation statement)

References 25 publications

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“…Since c-MET signaling is classically activated by the binding of its primary ligand, HGF, the receptor and the respective signaling pathway will therefore be overactivated under fibrotic conditions. In fact, this is corroborated by a recent study that confirms an upregulation of c-MET in the fibrotic foci either in IPF and fibrotic hypersensitivity pneumonia, reinforcing its importance in the pathogenesis of pulmonary fibrosis [ 75 ]. As such, the expression of c-MET in lung immune cells might be induced by HGF under fibrotic conditions, reinforcing their proinflammatory and cytotoxic capacities which contributes to the establishment of tissue fibrosis.…”

Section: Discussionsupporting

confidence: 65%

Deregulated immune cell recruitment orchestrated by c-MET impairs pulmonary inflammation and fibrosis

Barbosa-Matos,

Borges-Pereira,

Libório-Ramos

et al. 2024

Respir Res

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Background Pulmonary fibrosis (PF) represents the pathologic end stage of several interstitial lung diseases (ILDs) associated with high morbidity and mortality rates. However, current treatments can only delay disease progression rather than provide a cure. The role of inflammation in PF progression is well-established, but new insights into immune regulation are fundamental for developing more efficient therapies. c-MET signaling has been implicated in the migratory capacity and effector functions of immune cells. Nevertheless, the role of this signaling pathway in the context of PF-associated lung diseases remains unexplored. Methods To determine the influence of c-MET in immune cells in the progression of pulmonary fibrosis, we used a conditional deletion of c-Met in immune cells. To induce pulmonary fibrosis mice were administered with bleomycin (BLM) intratracheally. Over the course of 21 days, mice were assessed for weight change, and after euthanasia at different timepoints, bronchoalveolar lavage fluid cells and lung tissue were assessed for inflammation and fibrosis. Furthermore, c-MET expression was assessed in cryobiopsy sections, bronchoalveolar lavage fluid cells samples and single cell RNA-sequencing dataset from human patients with distinct interstitial lung diseases. Results c-MET expression was induced in lung immune cells, specifically in T cells, interstitial macrophages, and neutrophils, during the inflammatory phase of BLM-induced PF mouse model. Deletion of c-Met in immune cells correlated with earlier weight recovery and improved survival of BLM-treated mice. Moreover, the deletion of c-Met in immune cells was associated with early recruitment of the immune cell populations, normally found to express c-MET, leading to a subsequent attenuation of the cytotoxic and proinflammatory environment. Consequently, the less extensive inflammatory response, possibly coupled with tissue repair, culminated in less exacerbated fibrotic lesions. Furthermore, c-MET expression was up-regulated in lung T cells from patients with fibrosing ILD, suggesting a potential involvement of c-MET in the development of fibrosing disease. Conclusions These results highlight the critical contribution of c-MET signaling in immune cells to their enhanced uncontrolled recruitment and activation toward a proinflammatory and profibrotic phenotype, leading to the exacerbation of lung injury and consequent development of fibrosis.

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Section: Discussionsupporting

confidence: 65%