Up-regulation of a stress-responsive endochitinase VvChit-IV in grapevine cell cultures improves in vitro stress tolerance

Ben-Amar, Anis; Allel, Dorsaf; Mliki, Ahmed

doi:10.1007/s00709-021-01733-y

Cited by 10 publications

(5 citation statements)

References 52 publications

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“…This suggested that ethylene might participate in the regulation of fruit ripening by accelerating cell wall degradation. In plants, endochitinase and cell wall invertase, are a kind of pathogenesis-induced proteins, play an important role in defense mechanism and stress tolerance ( Hayes et al, 2010 ; Ben-Amar et al, 2022 ). Papaya is easily infected with a variety of pathogen-caused diseases.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Ubiquitylomic Analysis of the Dynamic Changes and Extensive Modulation of Ubiquitylation in Papaya During the Fruit Ripening Process

Bian

Huo

et al. 2022

Front. Plant Sci.

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Lysine ubiquitination is a highly conserved post-translational modification with diverse biological functions. However, there is little available information on lysine ubiquitination of non-histone proteins in papaya (Carica papaya L.). In total, 3,090 ubiquitination sites on 1,249 proteins with diverse localizations and functions were identified. Five conserved ubiquitinated K motifs were identified. Enrichment analysis showed that many Hsps were differentially ubiquitinated proteins (DUPs), suggesting an essential role of ubiquitination in degradation of molecular chaperone. Furthermore, 12 sugar metabolism-related enzymes were identified as DUPs, including an involvement of ubiquitination in nutrimental changes during the papaya ripening process. The ubiquitination levels of five fruit ripening-related DUPs, including one ethylene-inducible protein, two 1-aminocyclopropane-1-carboxylic acid oxidases, one endochitinase, and one cell wall invertase, were significantly changed during the ripening process. Our study extends the understanding of diverse functions for lysine ubiquitination in regulation of the papaya fruit ripening process.

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Section: Discussionmentioning

confidence: 99%

Quantitative Ubiquitylomic Analysis of the Dynamic Changes and Extensive Modulation of Ubiquitylation in Papaya During the Fruit Ripening Process

Bian

Huo

et al. 2022

Front. Plant Sci.

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“…Nonetheless, challenges like specificity and non-target effects necessitate comprehensive ecological impact assessments. Chitinase-mediated induced systemic resistance (ISR) involves the activation of a plant's defense mechanisms against a broad range of pathogens (Ben-Amar et al 2022 ; Chouhan et al 2023 ). This systemic response is triggered by chitin derivatives released from pathogen cell walls.…”

Section: Biotechnological Exploits Of Chitinasesmentioning

confidence: 99%

Chitinases: expanding the boundaries of knowledge beyond routinized chitin degradation.

Unuofin,

Odeniyi,

Majengbasan

et al. 2024

Environ Sci Pollut Res

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Chitinases, enzymes that degrade chitin, have long been studied for their role in various biological processes. They play crucial roles in the moulting process of invertebrates, the digestion of chitinous food, and defense against chitin-bearing pathogens. Additionally, chitinases are involved in physiological functions in crustaceans, such as chitinous food digestion, moulting, and stress response. Moreover, chitinases are universally distributed in organisms from viruses to mammals and have diverse functions including tissue degradation and remodeling, nutrition uptake, pathogen invasion, and immune response regulation. The discovery of these diverse functions expands our understanding of the biological significance and potential applications of chitinases. However, recent research has shown that chitinases possess several other functions beyond just chitin degradation. Their potential as biopesticides, therapeutic agents, and tools for bioremediation underscores their significance in addressing global challenges. More importantly, we noted that they may be applied as bioweapons if ethical regulations regarding production, engineering and application are overlooked.

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“…In this regard, plant chitinases conform large gene families, which are expressed under different biotic but also abiotic stresses 29 . Although plant chitinases are the major and best-characterized pathogen-related (PR) proteins due to their hydrolase activity that enables them to cleave chitin coming from arthropods or fungi, they are also involved in abiotic stress signalling, functioning at different stages of plant development 29 , 30 . More specifically, chitinases hydrolyse ß-1,4 bonds that link long-chain polymers of N-acetyl-D-glucosamine, which form chitin´s structure, the second most abundant biopolymer in nature after cellulose 31 .…”

Section: Introductionmentioning

confidence: 99%

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Poza-Viejo

Redondo‐Nieto

Matías

et al. 2023

Sci Rep

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Quinoa is an Andean crop whose cultivation has been extended to many different parts of the world in the last decade. It shows a great capacity for adaptation to diverse climate conditions, including environmental stressors, and, moreover, the seeds are very nutritious in part due to their high protein content, which is rich in essential amino acids. They are gluten-free seeds and contain good amounts of other nutrients such as unsaturated fatty acids, vitamins, or minerals. Also, the use of quinoa hydrolysates and peptides has been linked to numerous health benefits. Altogether, these aspects have situated quinoa as a crop able to contribute to food security worldwide. Aiming to deepen our understanding of the protein quality and function of quinoa seeds and how they can vary when this crop is subjected to water-limiting conditions, a shotgun proteomics analysis was performed to obtain the proteomes of quinoa seeds harvested from two different water regimes in the field: rainfed and irrigated conditions. Differentially increased levels of proteins determined in seeds from each field condition were analysed, and the enrichment of chitinase-related proteins in seeds harvested from rainfed conditions was found. These proteins are described as pathogen-related proteins and can be accumulated under abiotic stress. Thus, our findings suggest that chitinase-like proteins in quinoa seeds can be potential biomarkers of drought. Also, this study points to the need for further research to unveil their role in conferring tolerance when coping with water-deficient conditions.

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Up-regulation of a stress-responsive endochitinase VvChit-IV in grapevine cell cultures improves in vitro stress tolerance

Cited by 10 publications

References 52 publications

Quantitative Ubiquitylomic Analysis of the Dynamic Changes and Extensive Modulation of Ubiquitylation in Papaya During the Fruit Ripening Process

Quantitative Ubiquitylomic Analysis of the Dynamic Changes and Extensive Modulation of Ubiquitylation in Papaya During the Fruit Ripening Process

Chitinases: expanding the boundaries of knowledge beyond routinized chitin degradation.

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

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