Up-regulation of abscisic acid signaling pathway facilitates aphid xylem absorption and osmoregulation under drought stress

Guo, Huijuan; Sun, Yucheng; Peng, Xinhong; Wang, Qinyang; Harris, Marvin K.; Ge, Feng

doi:10.1093/jxb/erv481

Cited by 71 publications

(74 citation statements)

References 61 publications

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“…He et al [23] have shown that the activity of PAL (an enzyme involved in lignin synthesis) is induced in chrysanthemum by aphid feeding, which implies a contribution of lignin to aphid tolerance. Aphids need to access the phloem for nutrient and to the xylem to avoid an excessive build-up of osmotic pressure resulting from their consumption of sugar-rich phloem fluid [24]. Thus, the great lignification of the CmMYB19 over-expressors may make it more difficult for the aphid stylet to gain access, and thus could act as the brake on aphid multiplication observed in the CmMYB19 over-expressors.…”

Section: Discussionmentioning

confidence: 99%

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Wang

Sheng

Zhang

et al. 2017

IJMS

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Abstract:The gene encoding the MYB (v-myb avian myeloblastosis vira l oncogene homolog) transcription factor CmMYB19 was isolated from chrysanthemum. It encodes a 200 amino acid protein and belongs to the R2R3-MYB subfamily. CmMYB19 was not transcriptionally activated in yeast, while a transient expression experiment conducted in onion epidermal cells suggested that the CmMYB19 product localized to the nucleus. CmMYB19 transcription was induced by aphid (Macrosiphoniella sanborni) infestation, and the abundance of transcript was higher in the leaf and stem than in the root. The over-expression of CmMYB19 restricted the multiplication of the aphids. A comparison of transcript abundance of the major genes involved in lignin synthesis showed that CmPAL1 (phenylalanine ammonia lyase 1), CmC4H (cinnamate4 hydroxylase), Cm4CL1 (4-hydroxy cinnamoyl CoA ligase 1), CmHCT (hydroxycinnamoyl CoA-shikimate/quinate hydroxycinnamoyl transferase), CmC3H1 (coumarate3 hydroxylase1), CmCCoAOMT1 (caffeoyl CoA O-methyltransferase 1) and CmCCR1 (cinnamyl CoA reductase1) were all upregulated, in agreement with an increase in lignin content in CmMYB19 over-expressing plants. Collectively, the over-expression of CmMYB19 restricted the multiplication of the aphids on the host, mediated by an enhanced accumulation of lignin.

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Section: Discussionmentioning

confidence: 99%

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Wang

Sheng

Zhang

et al. 2017

IJMS

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“…On the other hand, ABA can suppress SA-dependent defenses (De Torres Zabala et al, 2009; Jiang et al, 2010; Cao et al, 2011). Concerning aphids, there are several reports that infestation induced ABA levels or ABA-regulated gene expression in Glycine max , M. truncatula , and A. thaliana (Studham and Macintosh, 2013; Guo et al, 2015; Sun et al, 2015; Hillwig et al, 2016). In contrast, another study showed that ABA levels in M. truncatula were not affected or even reduced by A. pisum feeding (Stewart et al, 2016) a pattern we also found in our study, where ABA levels in aphid-infested plants were generally lower or very similar than those in control plants.…”

Section: Discussionmentioning

confidence: 99%

“…However, ABA could play other roles in plant-aphid interactions. For instance, ABA-driven stomatal closure could be advantageous for aphids under dry conditions since it maintains plant turgor and so facilitates aphid feeding (Guo et al, 2015). However, by causing reductions in photosynthetic activity, ABA-induced closure of stomata could decrease the carbohydrate supply available to aphids.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Legume Defense Signaling Pathways by Native and Non-native Pea Aphid Clones

et al. 2016

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The pea aphid (Acyrthosiphon pisum) is a complex of at least 15 genetically different host races that are native to specific legume plants, but can all develop on the universal host plant Vicia faba. Despite much research, it is still unclear why pea aphid host races (biotypes) are able to colonize their native hosts while other host races are not. All aphids penetrate the plant and salivate into plant cells when they test plant suitability. Thus plants might react differently to the various pea aphid host races. To find out whether legume species vary in their defense responses to different pea aphid host races, we measured the amounts of salicylic acid (SA), the jasmonic acid-isoleucine conjugate (JA-Ile), other jasmonate precursors and derivatives, and abscisic acid (ABA) in four different species (Medicago sativa, Trifolium pratense, Pisum sativum, V. faba) after infestation by native and non-native pea aphid clones of various host races. Additionally, we assessed the performance of the clones on the four plant species. On M. sativa and T. pratense, non-native clones that were barely able to survive or reproduce, triggered a strong SA and JA-Ile response, whereas infestation with native clones led to lower levels of both phytohormones. On P. sativum, non-native clones, which survived or reproduced to a certain extent, induced fluctuating SA and JA-Ile levels, whereas the native clone triggered only a weak SA and JA-Ile response. On the universal host V. faba all aphid clones triggered only low SA levels initially, but induced clone-specific patterns of SA and JA-Ile later on. The levels of the active JA-Ile conjugate and of the other JA-pathway metabolites measured showed in many cases similar patterns, suggesting that the reduction in JA signaling was due to an effect upstream of OPDA. ABA levels were downregulated in all aphid clone-plant combinations and were therefore probably not decisive factors for aphid-plant compatibility. Our results suggest that A. pisum clones manipulate plant-defense signaling to their own advantage, and perform better on their native hosts due to their ability to modulate the SA- and JA-defense signaling pathways.

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“…These receptors inactivate plant PP2Cs, such as ABI1 and ABI2 , which are known to suppress ABA signalling (Ma et al 2009; Park et al 2009). Recently, it was shown that aphids feeding on A. thaliana and the legume Medicago truncatula increase ABA content in the plants (Guo et al 2016; Hillwig et al 2016). Furthermore, M. persicae aphid population development was negatively affected on ABA-deficient mutants compared to wild-type A. thaliana plants (Kerchev et al 2013; Hillwig et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Brevicoryne brassicae aphids interfere with transcriptome responses of Arabidopsis thaliana to feeding by Plutella xylostella caterpillars in a density-dependent manner

et al. 2016

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Plants are commonly attacked by multiple herbivorous species. Yet, little is known about transcriptional patterns underlying plant responses to multiple insect attackers feeding simultaneously. Here, we assessed transcriptomic responses of Arabidopsis thaliana plants to simultaneous feeding by Plutella xylostella caterpillars and Brevicoryne brassicae aphids in comparison to plants infested by P. xylostella caterpillars alone, using microarray analysis. We particularly investigated how aphid feeding interferes with the transcriptomic response to P. xylostella caterpillars and whether this interference is dependent on aphid density and time since aphid attack. Various JA-responsive genes were up-regulated in response to feeding by P. xylostella caterpillars. The additional presence of aphids, both at low and high densities, clearly affected the transcriptional plant response to caterpillars. Interestingly, some important modulators of plant defense signalling, including WRKY transcription factor genes and ABA-dependent genes, were differentially induced in response to simultaneous aphid feeding at low or high density compared with responses to P. xylostella caterpillars feeding alone. Furthermore, aphids affected the P. xylostella-induced transcriptomic response in a density-dependent manner, which caused an acceleration in plant response against dual insect attack at high aphid density compared to dual insect attack at low aphid density. In conclusion, our study provides evidence that aphids influence the caterpillar-induced transcriptional response of A. thaliana in a density-dependent manner. It highlights the importance of addressing insect density to understand how plant responses to single attackers interfere with responses to other attackers and thus underlines the importance of the dynamics of transcriptional plant responses to multiple herbivory.Electronic supplementary materialThe online version of this article (doi:10.1007/s00442-016-3758-3) contains supplementary material, which is available to authorized users.

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Up-regulation of abscisic acid signaling pathway facilitates aphid xylem absorption and osmoregulation under drought stress

Abstract: HighlightAphid fecundity is curtailed in drought-stressed plants primarily via reduced water status; however, activation of ABA signaling by drought cross-talks with SA and JA signaling to reduce induced plant resistance effects on aphid fecundity.

Cited by 71 publications

References 61 publications

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

CmMYB19 Over-Expression Improves Aphid Tolerance in Chrysanthemum by Promoting Lignin Synthesis

Modulation of Legume Defense Signaling Pathways by Native and Non-native Pea Aphid Clones

Brevicoryne brassicae aphids interfere with transcriptome responses of Arabidopsis thaliana to feeding by Plutella xylostella caterpillars in a density-dependent manner

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