Up-Regulation of Cell-Free MicroRNA-1 and MicroRNA-221-3p Levels in Patients with Myocardial Infarction Undergoing Coronary Angiography

Abstract

Purpose: Myocardial infarction (MI), known as a multifactorial disease, remains the predominant cause of mortality and sudden deaths annually. The current study aimed to measure the expression of microRNA-1 and microRNA-221-3p in MI patients. Methods: In the current study, 100 healthy controls (with no history of heart disease) and 200 patients with MI were selected. Patients were divided into two groups based on angiography results: normal (no significant artery stenosis) and primary percutaneous coronary intervention (primary PCI, significant artery stenosis). The levels of microRNA-1 and microRNA-221-3p were quantified using real-time quantitative polymerase chain reaction. The correlation between levels of microRNAs and the common cardiac markers were analyzed statistically. Results: In comparison to fold change, microRNA-1 elevations were 8.5-fold in normal patients and 60-fold in patients with primary PCI; while microRNA-221-3p levels were 210- fold higher in primary PCI and 31.31-fold higher in normal cases compared with the healthy controls. Receiver operating characteristic analysis showed that the area under the curve (AUC) for circulating microRNA-1 and microRNA-221 were 0.903 and 0.958 in normal patients and 0.927 and 0.985 in primary PCI patients (p <0.0001), respectively. Pearson correlation (ρ) analysis showed that circulation of microRNA-1 correlated with serum levels of cardiac troponin I (CTnI) (ρ=0.24), creatinine (ρ =0.34), creatinine kinase-myocardial band (CK-MB) (ρ=0.31), and microRNA-221-3p was significantly correlated with serum levels of CTnI (ρ =0.6), creatinine (ρ=0.41), and CK-MB (ρ=0.37), (P <0.0001). Conclusion: The study underscored the potential of microRNA-1 and microRNA-221-3p as informative biomarkers and positively correlated with artery stenosis in MI.

Diagnostic and prognostic value of plasma miR-106a-5p levels in patients with acute heart failure

“…This harsh reality forces researchers to conduct more investigations on AHF, especially in terms of diagnosis and prognosis. A great body of available literature has demonstrated the significance of miRNAs for the diagnosis and prognosis of heart diseases, including HF and myocardial infarction [ 29 – 34 ]. The salient findings from this study illustrated that plasma miR-106a-5p expression was reduced in AHF patients and could assist in diagnosing AHF and predicting the poor prognosis of AHF.…”

Survival of Myocardial Infarction Patients with Diabetes Mellitus at the Invasive Era (Results from the Városmajor Myocardial Infarction Registry)

“…Circulating miR-19a was found to have a prognostic value as a promising molecular target with acceptable sensitivity and specificity for the early diagnosis and prognosis of acute MI [14]. Moreover, the potential of microRNA-1 and microRNA-221-3p as informative biomarkers positively correlating with coronary artery stenosis in MI was found [15]. Moreover, the microRNA reducing effects of metformin can provide a potential therapeutic strategy for patients with type 2 diabetes by reducing the MI risk [16].…”

Bioinformatics and experimental analyses of glutamate receptor and its targets genes in myocardial and cerebral ischemia

“…Cardiac and cerebral damage caused by ischemic-hypoxic-reperfusion injury from cardiopulmonary resuscitation, severe compound injuries, and complex cardiovascular diseases are the major causes of acute mortality and chronic disability in patients [ 1 ]. This type of injury is an extremely complex pathophysiological cascade response process that includes a variety of damage repair mechanisms, such as neuronal autophagy, apoptosis, inflammatory damage, mitochondrial damage, and intracellular calcium overload [ 2 – 4 ]. Many studies have been conducted in the last decade on the treatment of ischemia-reperfusion injury in the heart and brain.…”