Up-regulation of<i>LSB1</i>/<i>GDU3</i>affects geminivirus infection by activating the salicylic acid pathway

Chen, Hao; Zhang, Zhonghui; Teng, Kunling; Lai, Jianbin; Zhang, Yiyue; Huang, Yajing; Li, Yin; Liang, Liming; Wang, Yiqin; Chu, Chengcai; Guo, Hui‐Shan; Xie, Qi

doi:10.1111/j.1365-313x.2009.04120.x

Cited by 74 publications

(60 citation statements)

References 56 publications

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“…Thus, we hypothesized that changes affecting host DNA methylation may also affect host antiviral defenses and geminivirus infectivity. To test this idea, we first compared the infectivity of c2 -mutant BSCTV to that of intact BSCTV using an A. tumefaciens spraying method described previously (Chen et al, 2010). The c2 -mutant BSCTV showed milder and delayed symptoms, such as longer inflorescence, mild curling inflorescence, and more production of lateral branches ( Figure 5A).…”

Section: Loss Of Function Of Samdc1 Has a Similar Effect On Virus Infmentioning

confidence: 99%

“…SD medium minus Leu and Trp ensure the maintenance of both expression plasmids. The interactions were confirmed by assessing b-galactosidase activity using a filter-lift assay (Breeden and Nasmyth, 1985) The construct containing full-length BSCTV C2 was generated by PCR using pCambia1300-BSCTV (1.8 copy) (Chen et al, 2010). Constructs containing defined regions of SAMDC1 were generated by different restriction endonuclease digestions of full-length SAMDC1 cDNA: truncation from amino acids 1 to 83 was produced by KpnI; truncation from amino acids 182 to 292 was produced by EcoRI and XhoI; truncation from amino acids 292 to 366 was produced by XhoI; and truncation from amino acids 226 to 366 was produced by EcoRI.…”

Section: Yeast Two-hybrid Screenmentioning

confidence: 99%

“…Rosette leaves of 4-week-old plants were agroinoculated with BSCTV using an established A. tumefaciens spraying method described previously (Chen et al, 2010). The concentration of the suspension of A. tumefaciens strain EHA105 (Hood et al, 1993) carrying pCambia1300-BSCTV (1.8 copy) or the C2 mutant was at a dose of OD 600 = 2.0.…”

Section: Bsctv Agroinoculationmentioning

confidence: 99%

“…Total DNA was extracted using a CTAB buffer as described (Chen et al, 2010). Genomic DNA was stained using ethidium bromide as a loading control.…”

Section: Dna Gel Blottingmentioning

confidence: 99%

“…Plants have evolved various mechanisms to respond to viral invasion, such as pathways mediated by gene silencing, salicylic acid, and metabolism regulation (Herbers et al, 1996;Vanitharani et al, 2005;Bisaro, 2006;Ascencio-Ibá ñ ez et al, 2008;Chen et al, 2010). Among them, gene silencing-mediated antiviral defense is a wellconserved and effective pathway.…”

Section: Introductionmentioning

confidence: 99%

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BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis

et al. 2011

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Plant viruses are excellent tools for studying microbial-plant interactions as well as the complexities of host activities. Our study focuses on the role of C2 encoded by Beet severe curly top virus (BSCTV) in the virus-plant interaction. Using BSCTV C2 as bait in a yeast two-hybrid screen, a C2-interacting protein, S-adenosyl-methionine decarboxylase 1 (SAMDC1), was identified from an Arabidopsis thaliana cDNA library. The interaction was confirmed by an in vitro pull-down assay and a firefly luciferase complemention imaging assay in planta. Biochemical analysis further showed that the degradation of the SAMDC1 protein was inhibited by MG132, a 26S proteasome inhibitor, and that C2 could attenuate the degradation of the SAMDC1 protein. Genetic analysis showed that loss of function of SAMDC1 resulted in reduced susceptibility to BSCTV infection and reduced viral DNA accumulation, similar to the effect of BSCTV C2 deficiency. Bisulfite sequencing analysis further showed that C2 deficiency caused enhanced DNA methylation of the viral genome in infected plants. We also showed that C2 can suppress de novo methylation in the FWA transgenic assay in the C2 transgene background. Overexpression of SAMDC1 can mimic the suppressive activity of C2 against green fluorescent protein-directed silencing. These results suggest that C2 interferes with the host defense mechanism of DNA methylation-mediated gene silencing by attenuating the 26S proteasome-mediated degradation of SAMDC1.

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Section: Loss Of Function Of Samdc1 Has a Similar Effect On Virus Infmentioning

confidence: 99%

Section: Yeast Two-hybrid Screenmentioning

confidence: 99%

Section: Bsctv Agroinoculationmentioning

confidence: 99%

“…Total DNA was extracted using a CTAB buffer as described (Chen et al, 2010). Genomic DNA was stained using ethidium bromide as a loading control.…”

Section: Dna Gel Blottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis

et al. 2011

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Functional role of geminivirus encoded proteins in the host: Past and present

Kamal,

Zafar,

Razzaq

et al. 2024

Biotechnology Journal

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During plant–pathogen interaction, plant exhibits a strong defense system utilizing diverse groups of proteins to suppress the infection and subsequent establishment of the pathogen. However, in response, pathogens trigger an anti‐silencing mechanism to overcome the host defense machinery. Among plant viruses, geminiviruses are the second largest virus family with a worldwide distribution and continue to be production constraints to food, feed, and fiber crops. These viruses are spread by a diverse group of insects, predominantly by whiteflies, and are characterized by a single‐stranded DNA (ssDNA) genome coding for four to eight proteins that facilitate viral infection. The most effective means to managing these viruses is through an integrated disease management strategy that includes virus‐resistant cultivars, vector management, and cultural practices. Dynamic changes in this virus family enable the species to manipulate their genome organization to respond to external changes in the environment. Therefore, the evolutionary nature of geminiviruses leads to new and novel approaches for developing virus‐resistant cultivars and it is essential to study molecular ecology and evolution of geminiviruses. This review summarizes the multifunctionality of each geminivirus‐encoded protein. These protein‐based interactions trigger the abrupt changes in the host methyl cycle and signaling pathways that turn over protein normal production and impair the plant antiviral defense system. Studying these geminivirus interactions localized at cytoplasm‐nucleus could reveal a more clear picture of host–pathogen relation. Data collected from this antagonistic relationship among geminivirus, vector, and its host, will provide extensive knowledge on their virulence mode and diversity with climate change.

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Curtovirus

Briddon¹,

Stenger²

2011

The Springer Index of Viruses

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Up-regulation ofLSB1/GDU3affects geminivirus infection by activating the salicylic acid pathway

Cited by 74 publications

References 56 publications

BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis

BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis

Functional role of geminivirus encoded proteins in the host: Past and present

Curtovirus

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