Up-Regulation of MUC18 in Airway Epithelial Cells by IL-13

Simon, Glenn C.; Martin, Richard J.; Smith, Sean; Thaikoottathil, Jyoti; Bowler, Russell P.; Barenkamp, Stephen J.; Chu, Hong Wei

doi:10.1165/rcmb.2010-0384oc

Cited by 30 publications

(29 citation statements)

References 33 publications

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“…A previous study suggests up-regulation of MUC18 expression in brushed bronchial epithelial cells from COPD patients [24], but MUC18 function in the lung remains unclear. We have found MUC18 up-regulation in airway epithelial cells as well as in lung macrophages from human asthmatics and COPD patients [13,14]. Importantly, we demonstrated a pro-inflammatory function of MUC18 in lung macrophages in the context of bacterial (e.g., mycoplasma) infection [14].…”

Section: Discussionmentioning

confidence: 89%

“…However, MUC18 regulation and function in inflammatory lung diseases such as asthma or COPD are poorly understood. Our recent publications [13,14] demonstrated MUC18 up-regulation in airway epithelial cells of asthmatics and COPD patients. Importantly, MUC18 is critical to bacteria-induced lung inflammation, which is in part through activation of NF-κB signaling [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Our recent publications [13,14] demonstrated MUC18 up-regulation in airway epithelial cells of asthmatics and COPD patients. Importantly, MUC18 is critical to bacteria-induced lung inflammation, which is in part through activation of NF-κB signaling [13,14]. However, whether MUC18 promotes inflammatory responses to Toll-like receptor (TLR) ligands mimicking viral infection or viruses has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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MUC18 Differentially Regulates Pro-Inflammatory and Anti-Viral Responses in Human Airway Epithelial Cells

Berman

Huang

Jiang

et al. 2014

J Clin Cell Immunol

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Objective MUC18 or CD146, a transmembrane glycoprotein, is mainly expressed by endothelial cells and smooth muscle cells where it serves as a cell-cell adhesion molecule. We have found MUC18 up-regulation in airway epithelial cells of patients with asthma and chronic obstructive pulmonary disease (COPD). However, the function of MUC18 in airway epithelial cells remains unclear. In the present study, we tested the hypothesis that MUC18 exerts a pro-inflammatory function during stimulation with a viral mimic polyI:C or human rhinovirus infection. Methods Normal human primary airway epithelial cells were transduced with lentivirus encoding MUC18 cDNA to over-express MUC18 or with GFP (control), and treated with polyI:C or HRV for detection of pro-inflammatory cytokine IL-8 and anti-viral gene IFN-β. Additionally, we performed cell culture of human lung epithelial cell line NCIH292 cells to determine the mechanisms of MUC18 function. Results We found that MUC18 over-expression promoted IL-8 production, while it inhibited IFN-β expression following polyI:C stimulation or HRV infection. Increased phosphorylation of MUC18 serines was observed in MUC18 over-expressing cells. Reduction of MUC18 serine phosphorylation by inhibiting ERK activity was associated with less production of IL-8 following polyI:C stimulation. Conclusions Our results for the first time demonstrate MUC18's pro-inflammatory and anti-viral function in human airway epithelial cells.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MUC18 Differentially Regulates Pro-Inflammatory and Anti-Viral Responses in Human Airway Epithelial Cells

Berman

Huang

Jiang

et al. 2014

J Clin Cell Immunol

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“…Serine and threonine are sites of O-linked glycosylation for the peptide backbone [28,29]. From the 22 known human mucin genes 16 mucins genes are expressed in the lung, of which Muc1, Muc4, Muc5AC, Muc5B, and Muc16 reveal the highest expression profiles [30][31][32][33][34]. There are two different types of mucins, the membrane-tethered mucins like Muc1, Muc4, and MucC16, which contribute to the periciliary layer, and the secreted gel-forming mucins like Muc5AC and Muc5B, which form the upper gel-layer and determine the mucus viscosity [28,[35][36][37].…”

Section: Journal Of Clinical and Cellular Immunologymentioning

confidence: 99%

Small Airways in Asthma: Distal is Different

Vock¹,

Lunding²,

Fehrenbach³

et al. 2017

J Clin Cell Immunol

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“…Sympathetic nerves release the catecholamine noradrenaline (NA). Catecholamines bind to adreno-MUC5AC, MUC5B, MUC7, MUC8, MUC11, MUC13, MUC15, MUC16, MUC18, MUC19, MUC20, MUC21, and MUC22) mucin genes are expressed (19,31,35,56,63,68). The predicted amino acid sequences of the cloned mucin genes revealed that there are 2 types of mucin groups in humans: secreted and membrane-tethered mucins.…”

Section: +mentioning

confidence: 99%

α1-Adrenoceptors relate Ca2+ modulation and protein secretions in rat lacrimal gland

et al. 2015

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Noradrenaline (NA) is a catecholamine with multiple roles including as a hormone and a neurotransmitter. Cellular secretory activities are enhanced by adrenergic stimuli as well as by cholinergic stimuli. The present study aimed to determine which adrenoceptors play a role in controlling intracellular calcium ion ([Ca 2+ ] i ) level in acinar cells of rat lacrimal glands. Expression of mRNA for adrenoceptor subtypes in the acinar cells was assessed using RT-PCR. ] i increase. The peroxidase activity was quantified as a measure of mucin secretion. Ca 2+ -dependent exocytotic secretion of peroxidase was detected in rat lacrimal glands. The RT-PCR results showed that MUC1, MUC4, MUC5AC, MUC5B, and MUC16 were expressed in acinar cells. These findings indicated that NA activates α1-adrenoceptors, which were found to be the main receptors in Ca 2+ -related cell homeostasis and protein (including mucin) secretion in lacrimal glands.The lacrimal gland is the main contributor to the aqueous component of the preocular tear film, which contains water, electrolytes, proteins, peroxidase, mucins, and lactoferrin. The lacrimal gland is composed of several cell types, including myoepithelial cells, ductal cells, goblet cells, and acinar cells, which are the major cell type constituting 80% of the gland. Acinar cells are highly polarized and joined by tight junctions at the luminal membrane, creating distinct basolateral and apical membranes (12). Goblet cells are a source of mucus in tears and secrete different types of mucins onto the ocular surface, especially in the conjunctiva. Although the number of goblet cells increases in the nasolacrimal duct, as the diameter of the nasolacrimal duct lumen is narrower than that of the lacrimal sac. Regulation of lacrimal gland fluid secretions is under neural control; activation of the sensory nerves in the cornea and conjunctiva initiates an afferent pathway leading to the central nervous system, which subsequently activates an efferent pathway that stimulates parasympathetic and sympathetic nerves, and ultimately, the lacrimal gland (32). The appropriate amount and composition of lacrimal gland fluid are crucial for a healthy, intact ocular surface (14). Sympathetic nerves release the catecholamine noradrenaline (NA). Catecholamines bind to adreno-

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Up-Regulation of MUC18 in Airway Epithelial Cells by IL-13

Cited by 30 publications

References 33 publications

MUC18 Differentially Regulates Pro-Inflammatory and Anti-Viral Responses in Human Airway Epithelial Cells

MUC18 Differentially Regulates Pro-Inflammatory and Anti-Viral Responses in Human Airway Epithelial Cells

Small Airways in Asthma: Distal is Different

<b>α1-Adrenoceptors relate Ca</b><sup><b>2+</b></sup><b> modulation and protein secretions in rat lacrimal </b><b>gland </b>

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