Up-regulation of neuropeptide Y Y4 receptor mRNA expression in the brainstem of refed rats following 48h of food deprivation: Effect of leptin

Yahya, Ayesha; Xiao, Chun; Chance, William T.; Sheriff, Sulaiman

doi:10.1016/j.peptides.2006.07.003

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…It is also worth noting that the regulation of feeding behavior involves changes in the expression of not only NPF but also its receptor in both vertebrates and invertebrates (Wu et al, 2005b ; Yahya et al, 2006 ; Lingo et al, 2007 ). Unfortunately, we did not observe a phenotypic change after NPFR knockdown due to the low gene silencing efficiency of NPFR dsRNA, although we attempted this experiment many times.…”

Section: Discussionmentioning

confidence: 99%

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Qu²,

Zhang

et al. 2018

Front. Physiol.

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Neuropeptide F (NPF) signaling systems are widespread and highly evolutionarily conserved from vertebrates to invertebrates. In fact, NPF has been identified in many insect species and plays regulatory roles in diverse physiological processes, such as feeding, learning, reproduction and stress responses. NPF operates by interacting with the NPF receptor (NPFR). Here, we characterized and determined the presumed role of NPF signaling in the wingless parthenogenetic pea aphid, Acyrthosiphon pisum. Quantitative real-time reverse transcription-PCR (qRT-PCR) revealed that the expression levels of both NPF and NPFR transcripts varied across developmental stages, which implies that the NPF signaling system might participate in the developmental regulation of aphid physiological processes or behaviors. The NPF transcript was mainly detected in the head but not in the gut, whereas the NPFR transcript was mainly detected in both the gut and head. In addition, the NPF transcript levels were markedly up-regulated in starved aphids compared with satiated aphids, and the transcript levels recovered after re-feeding. In contrast, the NPFR transcript levels remained stable in starved and re-fed aphids. Furthermore, RNAi knockdown by the injection of NPF dsRNA into wingless adult aphids significantly reduced their food intake. Further analysis of the modification of aphid feeding behavior on broad bean plants using electrical penetration graphs (EPGs) revealed that both the probing time and the total duration of phloem activity decreased significantly in the NPF treatment group. These results indicated a lower appetite for food after NPF knockdown, which could explain the reduction in aphid food intake. NPF silencing was also shown to reduce reproduction but not survival in aphids. Overall, the results of these experiments suggest that NPF plays an important role in regulation of feeding in A. pisum.

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Section: Discussionmentioning

confidence: 99%

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Qu²,

Zhang

et al. 2018

Front. Physiol.

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“…Total RNA was isolated using Tri reagent as previously described (23). Five micrograms of total RNA were used for cDNA synthesis using oligo (dT), SuperScript reverse transcriptase, and RNase inhibitor (Invitrogen Life Technologies).…”

Section: Real-time Reverse Transcription-pcrmentioning

confidence: 99%

“…Two microliters of cDNA were used to examine the expression of NPY, Y1R, Y2R, and Y5R in breast cancer cell lines. Real-time reverse transcription-PCR was carried out on a 96-well optical plate in MX-3000P (Stratagene) using the SYBR green-based kit (Brilliant QPCR Master Mix, Stratagene), as previously described in our laboratory (23,24). The PCR program consisted of an initial 10-min denaturation at 95°C, followed by 40 cycles of 1-min denaturation at 92°C, 1-min annealing at 64°C, and 1 min of extension at 72°C.…”

Section: Real-time Reverse Transcription-pcrmentioning

confidence: 99%

“…The expression of glyceraldehyde-3-phosphate dehydrogenase mRNA was used to normalize the RNA input. The threshold cycle (Ct) value analysis, PCR efficiency, linearity, slopes of the standard curve, relative quantity of fluorescence, and dissociation curve analysis were determined by the Stratagene software program (23,24).…”

Section: Real-time Reverse Transcription-pcrmentioning

confidence: 99%

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Neuropeptide Y Y5 Receptor Promotes Cell Growth through Extracellular Signal-Regulated Kinase Signaling and Cyclic AMP Inhibition in a Human Breast Cancer Cell Line

Sheriff

Ali

Yahya

et al. 2010

Molecular Cancer Research

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Overexpression of neuropeptide Y (NPY) and its receptor system has been reported in various types of cancers. NPY Y5 receptor (Y5R) has been implicated in cell growth and angiogenesis. However, the role of Y5R in breast cancer is unknown. To identify the role of Y5R in breast cancer, we screened several breast cancer cell lines to examine the expression of Y5R and its function in breast cancer. All screened cell lines express both Y1 receptor and Y5R except BT-549, which expresses mainly Y5R. Binding studies showed that NPY, Y5R-selective agonist peptide, and Y5R-selective antagonist (CGP71683A) displaced

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“…Brainstem Y 4 receptor mRNA levels respond to alterations in nutritional status or treatment with leptin in rats (152). Peripheral administration of leptin and refeeding after 48 h of fasting up-regulate Y 4 mRNA level in brainstem in rats.…”

Section: B Regulation Of Npy Receptors In Feeding Behavior and Energmentioning

confidence: 99%

Neuropeptide Y Receptor Selective Ligands in the Treatment of Obesity

Kamiji

Inui

2007

Endocrine Reviews

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Up-regulation of neuropeptide Y Y4 receptor mRNA expression in the brainstem of refed rats following 48h of food deprivation: Effect of leptin

Cited by 6 publications

References 28 publications

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Expression of Neuropeptide F Gene and Its Regulation of Feeding Behavior in the Pea Aphid, Acyrthosiphon pisum

Neuropeptide Y Y5 Receptor Promotes Cell Growth through Extracellular Signal-Regulated Kinase Signaling and Cyclic AMP Inhibition in a Human Breast Cancer Cell Line

Neuropeptide Y Receptor Selective Ligands in the Treatment of Obesity

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