Up-regulation of reactivity and survival genes in astrocytes after exposure to short duration overpressure

VandeVord, Pamela J.; Leung, Lai Yee; Hardy, Warren N.; Mason, Matthew J.; Yang, King H.; King, Albert I.

doi:10.1016/j.neulet.2008.01.056

Cited by 30 publications

(28 citation statements)

References 30 publications

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“…4,5 It has been assumed that shortduration overpressure is a dominating physical factor for TBI. 6,7 Especially, the shockwave of the blast TBI has a pulse duration of an order of milliseconds. 4 Based on this assumption and observation, barometer chamber capable of transient pressurization has been developed and used for assessing brain cell behavior under TBI conditions.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in the fluid percussion barotrauma chamber a pressure pulse with 20-30 ms long duration and a peak pressure of up to 0.5 MPa was used to examine the human glial cell behavior under TBI. 6 Recently, VandeVord et al 7 used metal ball striking barometer chamber to examine the astrocyte cell behavior under TBI but the metal ball striking produced multiple pressure pulses. In this study, we have developed a novel cell pressurization device that generates single-pulse type impulsive pressurization.…”

Section: Discussionmentioning

confidence: 99%

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Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study

Nienaber

Lee

Feng

et al. 2011

JoVE

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A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration. Video LinkThe video component of this article can be found at https://www.jove.com/video/2723/ Protocol 1. Neuronal Cell Culture 1. Brain cells including neuronal cells, astrocytes, and their co-culture may be used as a cell model. As a feasibility demonstration, impulsive pressurization of cell-line neuronal cells is presented. 2. SH-SY5Y human neuroblastoma cells (ATCC, CRL-2266) are cultured on 18 mm diameter glass coverslips. Cells are seeded at a density of 3×10 3 cells/cm 2 using the growth media composed of DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. Cell cultured glass slides are kept in a 5% CO 2 humidified incubator at 37°C. 3. To obtain the neuronal cell differentiation of SH-SY5Y cells, cells are treated with media further supplemented with 10 μM retinoic acid (RA) for 7 days with media changed every two days. On day 7, cells are ready to be pressurized. Pressurization Equipment: Kolsky Bar1. The Kolsky bar, developed by Kolsky 1 in 1949, has been used to measure the mechanical property of a material at a very high loading rate.The apparatus consists of two bars with a sample placed in contact between the bars. A stress wave, created on the incident bar, propagates to the sample where the wave splits into a reflected and transmitted wave. The stress in the sample is proportional to the transmitted wave. In this study, we utilize a cell pressurization chamber to be placed between the two bars of the Kolsky set-up. 2. The two aluminum alloy bars (each 6-m long) are suspended by aligned brass bearings, and an in vitro cell pressurization chamber is sandwiched between the bars. The upstream bar is the incident bar with a 130 lb mass clamped onto one end. A friction clamp is placed at a position that will give the desired pulse duration. By engaging the clamp and tightening a scissors jack between the mass and a loading support, the clamp-mass section of the incident bar is pre-compressed to the desired level. Forcing a notched bolt that locks the clamp to a sudden break with ...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study

Nienaber

Lee

Feng

et al. 2011

JoVE

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“…RT of 1 μg RNA was carried out for 60 min at 42°C in 20 μl reaction buffer (50 mM Tris-Hcl, pH 8.3) containing KCl (50 mM), MgCl 2 (5 mM), Oligo(dt) 18 Primer (20 mM), and dNTP mix (20 μM each) in the presence of RevertAid TM M-MuLV Reverse Transcriptase (200 U). Polymerase chain reaction (PCR) reactions were performed under the following conditions: Primers for PCR amplification were as follows: glyceraldehyde 3-phosphate dehydrogenase (GAPDH), BLBP, GFAP, vimentin, nestin, and Sox2 (Kwitek et al 2004;Yang et al 2007;Vandevord et al 2008). Details were summarized in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Generation and identification of rat fetal cerebral radial glia-like cells in vitro

Jin

Qin

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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The role of radial glia cells (RGCs) as neural progenitors and as guides for migrating neurons is well established, mouse or human-derived radial glia (RG)-like cells in vitro showed some astroglia and stem/progenitor properties like RGCs in vivo, but different species-derived RG-like cells present some different properties. Here we acquired rat-derived RG-like cells on adherent conditions in vitro and then identified their astroglia and stem/progenitor properties. Similarly to the RGCs, the RG-like cells could be double-labeled by brain lipid-binding protein, glial fibrillary acidic protein, vimentin with nestin and expressed some astroglia and stem/progenitor genes; these cells also presented tripotent differentiation potentialities, albeit the ability of gliogenesis far exceeded the neurogenesis in vitro. Taken together, we acquired and identified some properties of rat-derived RG-like cells from fetal cerebral cortices in vitro.

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“…4.4. This example from Vandevord et al [30] consists of a five-piece aluminium chamber. The chamber contains a pedestal in the centre for housing cell cultures.…”

Section: Pressure Systemsmentioning

confidence: 99%

“…The first pulse is of a high amplitude and short duration and the sum of all the integrated positive components of the pulse sequence is the time integral of the pressure, referred to as the 'positive impulse'. In Vandevord et al [30] this system was used to examine the effects of short duration overpressures on adherent cultures of rat astrocytes. In this setup, Petrie dishes containing cell culture were filled with culture media, covered with Parafilm so that no air bubbles are present, and placed on the pedestal.…”

Section: Pressure Systemsmentioning

confidence: 99%

Blast Loading of Cells

Brown

2016

Blast Injury Science and Engineering

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Up-regulation of reactivity and survival genes in astrocytes after exposure to short duration overpressure

Cited by 30 publications

References 30 publications

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study

Impulsive Pressurization of Neuronal Cells for Traumatic Brain Injury Study

Generation and identification of rat fetal cerebral radial glia-like cells in vitro

Blast Loading of Cells

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