Up-scaling capillary zone electrophoresis separations of polydisperse anionic polyelectrolytes with preparative free-flow electrophoresis exemplified with a soil fulvic acid

Junkers, Jens; Schmitt‐Kopplin, Philippe; Munch, J. C.; Kettrup, A.

doi:10.1002/1522-2683(200209)23:17<2872::aid-elps2872>3.0.co;2-5

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…Correlation of CZE and FFZE has been utilized for conversion of analytical CZE separations into preparative FFZE processes also for some other substances. A good correlation has been found between CZE and FFZE separations of model compounds, carboxylic acids and polystyrene sulfonates, as well as polydisperse anionic polyelectrolyte, soil fulvic acid 77. Diffusion and electrohydrodynamic distortions were more pronounced in FFZE than in CZE, but comparison of FFZE fractions with CZE measurements of the same sample using the effective mobility scale showed a good agreement of both methods and confirmed the possibility to scale‐up analytical CZE separation into preparative FFZE on the basis of their correlation.…”

Section: Correlation Of Ce and Ffe – Basis For Conversion Of Ce To Ffementioning

confidence: 58%

From micro to macro: Conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free‐flow electrophoresis scale

Kašička

2009

Electrophoresis

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This invited contribution in the special issue of Electrophoresis published in celebration of the 30th Anniversary of this journal reflects the impact of our milestone paper [Prusík, Z., Kasicka, V., Mudra, P., Stepánek, J., Smékal, O., Hlavácek, J., Electrophoresis 1990, 11, 932-936] in the area of conversion of microscale analytical and micropreparative CE separations of biomolecules and bioparticles into (macro)preparative free-flow electrophoresis (FFE) scale on the basis of a correlation between CE and FFE methods. In addition to the survey of advances in the relatively narrow field of CE-FFE correlation and CE-FFE conversion, a comprehensive review of the recent developments of micropreparative CE and (macro)preparative FFE techniques is also presented and applications of these techniques to micro- and (macro)preparative separations and purifications of biomolecules and bioparticles are demonstrated. The review covers the period since the year of publication of the above paper, i.e. ca. the last 20 years.

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Section: Correlation Of Ce and Ffe – Basis For Conversion Of Ce To Ffementioning

confidence: 58%

From micro to macro: Conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free‐flow electrophoresis scale

Kašička

2009

Electrophoresis

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“…To overcome sample limitations, a preparative approach of CZE was proposed with free-flow electrophoresis (FFE) for the structure characterization of dissolved natural organic matter [335]; combined off-line with flowinjection ESI-MS, the CZE-ESI-MS of the same materials could be validated [336]. These are probably exciting fields of investigation with many challenges for the next decades.…”

Section: Where Do We Go?mentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

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Capillary electrophoresis -mass spectrometry: 15 years of developments and applicationsSince its introduction in 1987, capillary electrophoresis-mass spectrometry (CE-MS) has developed to a well accepted multidimensional analytical approach complementary and/or competitive to classical MS-hyphenated separation techniques. The threefold combination of rapid developments of an exceptional separation technique, of selective mass detection possibilities, and of very mild ionization modes first allowed these progresses. This article shows the CE specificities that need to be well controlled/known, compared to classical and more routinely used liquid chromatography in the light of its coupling to MS. The major trends and developments over the last 15 years and most of the reviews and applications found in ISI Web of science and publisher databases are presented in a tabulated way. The reader can thus rapidly find existing CE-MS analysis techniques in his field of research and application (forensics, environment, bioanalytics, pharmaceutics, and metabolites).

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“…This new scaling enables the direct comparison of complete electropherograms 55. The procedure was described elsewhere 52, 54. Briefly, after the essential parameters (voltage, buffer flow rate and buffer ionic strength) were optimized, these settings were used to verify the behavior of the model compounds (benzoic acid (−0.0186 cm 2 /Vmin), phthalic acid (−0.030 cm 2 /Vmin), trimellitic acid (−0.038 cm 2 /Vmin), and pyromellitic acid (−0.042 cm 2 /Vmin)) and their effective mobilities, which were described formally with CZE system.…”

Section: Methodsmentioning

confidence: 99%

“…Free‐flow electrophoresis (FFE) is successfully used as a zone electrophoretic technique for peptides, membrane proteins, biological particles, and for small ions 51. NOM also was separated previously by means of FFE, based on their chemical properties (charge) and configuration (size) under different solution conditions with minimal wall interaction as compared with LC 52. Furthermore, FFE separation produces sizable amounts of sample that could be further investigated with methods like 1 H or 13 C NMR, where higher amounts of sample are required (results not shown here).…”

Section: Introductionmentioning

confidence: 96%

Preparative free‐flow electrophoretic offline ESI‐Fourier transform ion cyclotron resonance/MS analysis of Suwannee River fulvic acid

et al. 2010

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Free-flow electrophoresis (FFE), a preparative free zone electrophoretic method, was used offline in conjunction with ultrahigh-resolution FT/ion cyclotron resonance -MS to resolve the complexity of Suwannee River fulvic acid (SRFA). Before MS, the FFE separation conditions and the compatibility with ESI were optimized. The constituents in SRFA were effectively separated based on their charge states and sizes. The obtained mass spectra were compared by means of van Krevelen diagrams and the calculated aromaticity indices of the individual constituents were used to describe the distribution of aromatic/unsaturated structures across the FFE-fractionated samples. The consolidated number of ions observed within the individual SRFA fractions were much higher than those of the bulk samples alone, demonstrating extensive ion suppression effects in bulk SRFA likely also operating in the analysis of complex biogeochemical mixtures in flow injection mode. The FFE approach allows for producing sizable amounts of sample from dilute solutions, which can be easily fractionated into dozens of individual samples with the possibility of further in-depth characterization.

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Up-scaling capillary zone electrophoresis separations of polydisperse anionic polyelectrolytes with preparative free-flow electrophoresis exemplified with a soil fulvic acid

Cited by 10 publications

References 29 publications

From micro to macro: Conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free‐flow electrophoresis scale

From micro to macro: Conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free‐flow electrophoresis scale

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Preparative free‐flow electrophoretic offline ESI‐Fourier transform ion cyclotron resonance/MS analysis of Suwannee River fulvic acid

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