Upconversion Luminescence Sensitized pH-Nanoprobes

Mahata, Manoj Kumar; Bae, Hyeongyu; Lee, Jun Young

doi:10.3390/molecules22122064

Cited by 45 publications

(16 citation statements)

References 111 publications

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“…Molecular imaging can in principle provide powerful tools for identifying cancers with greatly improved specificity and sensitivity [5], and functional inorganic nanoparticles have shown great potential as imaging probes owing to the intrinsic physical properties of inorganic particles apart from the well-known enhanced permeation and retention effect of tumors for nano-objects. Among different kinds of functional inorganic nanoparticles, upconversion nanoparticles (UCNPs) are superior as light-emitting, and rareearth ions can facilely be combined with paramagnetic ones such as Gd 3+ , for simultaneously visualizing tumors through upconversion luminescence (UCL) [6] and magnetic resonance imaging (MRI) [7,8]. Besides, they also provide an excellent platform to further combine photoacoustic imaging (PAI) [9], single-photon emission computed tomography (SPECT) [10], and positron emission tomography (PET) imaging [11], with above imaging modalities.…”

Section: Introductionmentioning

confidence: 99%

Ultra-sensitive Nanoprobe Modified with Tumor Cell Membrane for UCL/MRI/PET Multimodality Precise Imaging of Triple-Negative Breast Cancer

Fang

Liu

et al. 2020

Nano-Micro Lett.

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HIGHLIGHTS• A biomimetic nanoprobe was built with cancer cell membrane-coated and Gd 3+ -doped upconversion nanoparticles.• The nanoprobe could be applied to in vivo UCL/MRI/PET multimodality precise imaging and successfully differentiated MDA-MB-231 tumor models through in vivo tri-modality imaging, which may be used for breast cancer molecular classification.ABSTRACT Triple-negative breast cancer (TNBC) is a subtype of breast cancer in which the estrogen receptor and progesterone receptor are not expressed, and human epidermal growth factor receptor 2 is not amplified or overexpressed either, which make the clinical diagnosis and treatment very challenging. Molecular imaging can provide an effective way to diagnose TNBC. Upconversion nanoparticles (UCNPs), are a promising new generation of molecular imaging probes. However, UCNPs still need to be improved for tumor-targeting ability and biocompatibility.This study describes a novel probe based on cancer cell membrane-coated upconversion nanoparticles (CCm-UCNPs), owing to the low immunogenicity and homologous-targeting ability of cancer cell membranes, and modified multifunctional UCNPs. This probe exhibits excellent performance in breast cancer molecular classification and TNBC diagnosis through UCL/MRI/PET tri-modality imaging in vivo. By using this probe, MDA-MB-231 was successfully differentiated between MCF-7 tumor models in vivo. Based on the tumor imaging and molecular classification results, the probe is also expected to be modified for drug delivery in the future, contributing to the treatment of TNBC. The combination of nanoparticles with biomimetic cell membranes has the potential for multiple clinical applications.

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Section: Introductionmentioning

confidence: 99%

Ultra-sensitive Nanoprobe Modified with Tumor Cell Membrane for UCL/MRI/PET Multimodality Precise Imaging of Triple-Negative Breast Cancer

Fang

Liu

et al. 2020

Nano-Micro Lett.

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“…Due to their multicolor emission, UCNPs are principally ideal energy donors in two component sensing systems. The combination of UCNPs and pH-sensitive dyes as energy acceptors, for instance, can yield ratiometric pH-sensors utilizing either resonance energy transfer (RET) processes − or reabsorption-related inner filter effects. − As energy acceptor, typically an analyte-responsive organic dye is used. For both sensor principles, the absorption of the indicator dye must overlap with one of the UCL bands, typically the green and blue emission for Er 3+ - and Tm 3+ -based UCNPs, and referencing to an analyte-inert UCL band is essential.…”

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confidence: 99%

Simple Self-Referenced Luminescent pH Sensors Based on Upconversion Nanocrystals and pH-Sensitive Fluorescent BODIPY Dyes

et al. 2019

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Optical probes for monitoring, imaging, and sensing of pH are of great interest for the scientific community as pH is a crucial marker for many processes in biotechnology, biology, medical diagnostics, biomedical research, and material corrosion. Thereby, optical pH sensors based on fluorescence have attracted interest in particular as fluorescence offers a high sensitivity down to the single molecule level, can be read out with relatively simple and readily miniaturized instrumentation, and allows online in situ measurements. Also the versatility ranging from molecular and nanosensor formats to planar optodes and fiber-optic sensors, and the non-invasive, non-destructive, and contactless nature of the measurement are application-friendly features. The information content, which is offered by a fluorescence intensity-based sensor, is usually unspecific and limited on the presence or the absence of the chromophore or analyte and can additionally be hampered by fluctuation of the excitation light intensity and changes in fluorophore concentration, e.g., due to photobleaching. Therefore, many fluorescence sensors are utilized in referenced systems, which enable twowavelength ratiometric measurements of the fluorescence intensity by the introduction of an analyte-inert reference with a spectrally distinguishable emission. This work presents the rational design of a versatile, modular, multi-component-based platform for ratiometric optical analyte sensing that can be simply adapted to different formats and measurement geometries. Therefore, readily available analyte-responsive fluorescent boron-dipyrromethene (BODIPY) dyes and near infrared (NIR)-excitable multicolour-Partikelgrößen wurden dafür via Transmissionselektronenmikroskopie (TEM) und Kleinwinkel-Röntgenstreuung (SAXS) bestimmt. Neben der Partikelgröße konnten durch die TEM-Messungen auch Informationen über die Kristallphasen der Nanopartikel erhalten werden. Neben der Erfassung des Partikelwachstums wurde die UCL der UCNPs für die ratiometrische Sensorplattform als Nanolampe und gleichzeitig als Referenzsignal verwendet. Die blaue Upconversion(UC)-Emission der NaYF 4 :Yb 3+ /Tm 3+ UCNPs wurde dabei zur Anregung der pH-sensitiven BODIPY-Farbstoffe verwendet, während die rote UC-Emission als inertes Referenzsignal verwendet wurde. Die Berechnung des Verhältnisses der Emissionsintensitäten der grünen Fluoreszenz des Farbstoffs und der roten UC-Emission des Partikels ermöglicht eine Bestimmung des pH-Werts. Das Potenzial dieser Strategie zur Erfassung des pH-Werts wurde beispielhaft für die Bestimmung der zeitabhängigen Änderungen des pH-Werts einer metabolisierenden Escherichia coli (E. coli)-Suspension gezeigt.

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“…The SPT of nanoparticles at the cellular level can be engineered for efficient drug or gene delivery and sensing [3,4,5,6,60,64,89]. For example, the information from SPT analysis provides the endocytosis, delivery pathway, and arrival at the target [14,57].…”

Section: Applicationsmentioning

confidence: 99%

“…The synthesis and characterization of lanthanide-doped upconversion nanoparticles (UCNPs) are well established [1,2,3,4,5,6]. In particular, UCNPs, which are doubly doped by the sensitizer cations (usually Yb 3+ ) and the activator cations (e.g., Yb 3+ , Er 3+ , Tm 3+ , Nd 3+ , Ho 3+ ) in the host material (e.g., NaYF 4 , NaGdF 4 ), have attracted the greatest interest [4,5]. By selecting a proper cation pair and adjusting their relative composition, their optical properties can be optimized, such as wavelengths, band-width, quantum yield, and photo stability [1,4].…”

Section: Introductionmentioning

confidence: 99%

“…By selecting a proper cation pair and adjusting their relative composition, their optical properties can be optimized, such as wavelengths, band-width, quantum yield, and photo stability [1,4]. UCNPs are excited by near-infrared (NIR) lasers, whose wavelength is well matched to the absorption wavelength of Yb 3+ (980 nm) and Nd 3+ (808 nm) [1,2,3,4,5]. In this NIR range, biomolecules show minimum absorption in vitro and in vivo [7,8,9,10,11,12].…”

Section: Introductionmentioning

confidence: 99%

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Two-Dimensional and Three-Dimensional Single Particle Tracking of Upconverting Nanoparticles in Living Cells

Shin

Song

Goh

et al. 2019

IJMS

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Lanthanide-doped upconversion nanoparticles (UCNPs) are inorganic nanomaterials in which the lanthanide cations embedded in the host matrix can convert incident near-infrared light to visible or ultraviolet light. These particles are often used for long-term and real-time imaging because they are extremely stable even when subjected to continuous irradiation for a long time. It is now possible to image their movement at the single particle level with a scale of a few nanometers and track their trajectories as a function of time with a scale of a few microseconds. Such UCNP-based single-particle tracking (SPT) technology provides information about the intracellular structures and dynamics in living cells. Thus far, most imaging techniques have been built on fluorescence microscopic techniques (epifluorescence, total internal reflection, etc.). However, two-dimensional (2D) images obtained using these techniques are limited in only being able to visualize those on the focal planes of the objective lens. On the contrary, if three-dimensional (3D) structures and dynamics are known, deeper insights into the biology of the thick cells and tissues can be obtained. In this review, we introduce the status of the fluorescence imaging techniques, discuss the mathematical description of SPT, and outline the past few studies using UCNPs as imaging probes or biologically functionalized carriers.

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Upconversion Luminescence Sensitized pH-Nanoprobes

Cited by 45 publications

References 111 publications

Ultra-sensitive Nanoprobe Modified with Tumor Cell Membrane for UCL/MRI/PET Multimodality Precise Imaging of Triple-Negative Breast Cancer

Ultra-sensitive Nanoprobe Modified with Tumor Cell Membrane for UCL/MRI/PET Multimodality Precise Imaging of Triple-Negative Breast Cancer

Simple Self-Referenced Luminescent pH Sensors Based on Upconversion Nanocrystals and pH-Sensitive Fluorescent BODIPY Dyes

Two-Dimensional and Three-Dimensional Single Particle Tracking of Upconverting Nanoparticles in Living Cells

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