Upconversion Nanoparticles-Based Fluorescence Immunoassay for the Sensitive Detection of 2-Amino-3-methylimidazo [4,5-f] Quinoline (IQ) in Heat Processed Meat

Abstract

A competitive fluorescence immunoassay for the quantitative detection of 2-amino-3-methylimidazo [4, 5-f] quinoline (IQ) in pan-fried meat patties was developed, using magnetic nanoparticles coupled with coating antigen as the capture probe and anti-IQ antibody coupled with NaYF4: Yb, Er upconversion nanoparticles as the signal probe. Under optimal conditionals, the wide detection range for IQ in phosphate buffer saline is from 0.01 to 100 μg·L−1 (R2 = 0.991) with a detection limit of 0.007 μg·L−1. This proposed method has been applied to detect IQ in two different types of pan-fried meat patties at varying frying times, and the IQ content in chicken patties and fish patties are 2.11–3.47 μg·kg−1 and 1.35–2.85 μg·kg−1, respectively. These results are consistent with that of the ultraperformance liquid chromatography-tandem mass spectrometry. In summary, this method can serve as a sensitive and specific test tool for the determination of IQ in processed meat.

A Comprehensive Review on Upconversion Nanomaterials-Based Fluorescent Sensor for Environment, Biology, Food and Medicine Applications

“…The experiments show that quantum dots are the most sensitive of norfloxacin, while the UCNPs provide the most accurate analysis results in the milk. In order to detect heterocyclic aromatic amine carcinogens in fried meat products, Huang et al [ 198 ] used NaYF 4 : Yb, Er grafted with anti-2-amino-3-methylimidazo [4, 5-f] quinoline (IQ) antibodies as fluorescent sensor through immunofluorescence sensing to detect IQ, and the LOD was as low as 7 ng L −1 .…”