Update of genetic information for the white-clawed crayfish in Spain, with new insights into its population genetics and origin

Matallanas, Beatriz; Ochando, M. D.; Alonso, F.; Callejas, C.

doi:10.1007/s13127-016-0268-4

Cited by 10 publications

(26 citation statements)

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“…For the last few years, the genetic variability of Spanish white-clawed crayfish has been assessed with mitochondrial and nuclear markers by sampling a significant number of populations throughout its distribution range in the country (Beroiz et al, 2008;Matallanas et al, 2013Matallanas et al, , 2016PedrazaLara et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish

Matallanas

Ochando

Callejas

2016

Knowl. Manag. Aquat. Ecosyst.

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-The white-clawed crayfish is endemic to western and southern Europe and its population has dramatically decreased in the last few decades. The latest reports on this species have shown that the genetic variability of Spanish populations displays a clear geographic pattern, with two main genetic groups across its range of distribution. To ensure the preservation of this endangered species, specimens for restocking purposes should be chosen, ideally, accordingly to their genetic characteristics. Here, we propose a PCR-RFLP assay developed for this purpose. Digestion of a fragment of the mitochondrial cytochrome oxidase subunit I gene -with two restriction enzymes, HpaI and CviAII -differentiates the most common haplotypes identified in Spain. This technique is a useful, low-cost and rapid method to facilitate the genetic characterization of Spanish white-clawed crayfish and, in doing so, to incorporate genetic information into conservation plans for this species. Key-words:Spanish white-clawed crayfish / Cytochrome oxidase subunit I (COI) / PCR-RFLP / Restriction enzymes / genetic groups Résumé -Mise au point d'un essai COI PCR-RFLP pour une caractérisation génétique rapide de l'écrevisse à pattes blanches espagnole. L'écrevisse à pattes blanches est endémique de l'Europe occidentale et méridionale et ses populations ont considérablement diminué au cours des dernières décennies. Les derniers rapports sur cette espèce ont montré que la variabilité génétique des populations espagnoles affiche une répartition géographique claire avec deux principaux groupes génétiques dans son aire de distribution. Pour assurer la conservation de cette espèce en voie de disparition, les échantillons à des fins de repeuplement doivent être choisis, idéalement, en fonction de leurs caractéristiques génétiques. Nous proposons ici une analyse PCR-RFLP développée à cet effet. La digestion d'un fragment du gène de la sous-unité I de cytochrome oxydase mitochondriale -avec deux enzymes de restriction HpaI et CviAII -différencie les haplotypes les plus communs identifiés en Espagne. Cette technique est une méthode, à faible coût, utile et rapide pour faciliter la caractérisation génétique de l'écrevisse à pattes blanches espagnole et, ce faisant, pour incorporer l'information génétique dans les plans de conservation pour cette espèce.

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Section: Discussionmentioning

confidence: 99%

Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish

Matallanas

Ochando

Callejas

2016

Knowl. Manag. Aquat. Ecosyst.

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“…

11recently published (Matallanas et al, 2016). Our work had concluded that the knowledge 12 generated by disparate scientific disciplines robustly converged to indicate that

Austropotamobius italicus is non-native species in Spain, but Matallanas et al (2016)

First, we point out that the mono-disciplinary approach followed by Matallanas et al (2016) is 17 much weaker than the transdisciplinary approach used and advocated by Clavero et al (2016).

Second, we believe that the genetic patterns presented by Matallanas et al (2016)

…”

mentioning

confidence: 85%

“…First, we point out that the mono-disciplinary approach followed by Matallanas et al (2016) is 17 much weaker than the transdisciplinary approach used and advocated by Clavero et al (2016).…”

mentioning

confidence: 92%

Multiple, solid evidence support that Austropotamobius italicus is not native to Spain

Clavero

Centeno‐Cuadros

2016

Org Divers Evol

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11recently published (Matallanas et al., 2016). Our work had concluded that the knowledge 12 generated by disparate scientific disciplines robustly converged to indicate that 13Austropotamobius italicus is non-native species in Spain, but Matallanas et al. (2016) 16First, we point out that the mono-disciplinary approach followed by Matallanas et al. (2016) is 17 much weaker than the transdisciplinary approach used and advocated by Clavero et al. (2016). 18Second, we believe that the genetic patterns presented by Matallanas et al. (2016)

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“…Later genetic studies have shown that A. astacus has inhabited Swedish aquatic ecosystems since the last ice age, thus being native to Sweden (Edsman et al, 2002;Gross et al, 2013;Dannewitz et al, 2021). Similarly, A. pallipes has been claimed to have been introduced to Spain from Italy only in the 1500s (Clavero et al, 2016), despite genetic evidence dating the species origin on the Iberian Peninsula from the last glaciation (Matallanas et al, 2011(Matallanas et al, , 2016Jelić et al, 2016;Martín-Torrijos et al, 2021a). Such scenarios, of course, create confusion and employ everyone to waste resources in order to correct the deliberate misinterpretation in any given case.…”

Section: Twisting the Definitions And Creating New Language To Cause Confusionmentioning

confidence: 99%

Money Kills Native Ecosystems: European Crayfish as an Example

et al. 2021

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Native European crayfish conservation was triggered by invasion of crayfish plague disease agent, Aphanomyces astaci, starting 1860s in Northern Italy. Resulting crayfish plague epidemics quickly spread over Continental Europe, then to Finland, Sweden and finally, after running amok around Europe, A. astaci was discovered also in Iberian Peninsula, Norway, Ireland, and United Kingdom in 1970s and 1980s. By that time significant proportion of native crayfish stocks had been lost, and while crayfish plague epidemics were still recorded, also industrialization and waterways construction were causing damage to remaining native crayfish stocks. While alien crayfish introductions, at least Faxonius limosus, already gave rise to first wave of crayfish plague epidemics in late 19th century, later in 1960s it was decided that introductions of alien Pacifastacus leniusculus should be initiated to replace native European crayfish populations. Decisions were based on presumed advantages for fishery, suitable habitat requirements and supposed immunity against A. astaci. Furthermore, conservation of native European crayfish species was sidelined and focus shifted toward alien crayfish stocking routine and consumption. Alien crayfish species introductions resulted in repeated waves of crayfish plague epidemics among remaining native crayfish stocks. It was soon discovered that alien crayfish of North American origin were, as suspected, permanent reservoirs for A. astaci, that some of those alien species were losing their resistance against selected strains of A. astaci and struggled in European aquatic ecosystems. In this article, we introduce numerous motives behind grand mistake of introducing alien crayfish species to Europe and then promoting their stocks instead of focusing on conservation of native crayfish species. We outline how false economical, biological and ecologic assumptions were used to justify a hasty introduction of alien crayfish, which has further devastated native crayfish and also permanently changed European aquatic ecosystems, both with disastrous consequences. Lesson to be learnt is that science-based warnings about alien species damage to native ecosystems and native crayfish must be taken with utmost caution. Protection of native European crayfish should be core issue, not commercial activities. Finally, we summarize main threats and actions needed to protect remaining native freshwater crayfish fauna in Europe.

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Update of genetic information for the white-clawed crayfish in Spain, with new insights into its population genetics and origin

Cited by 10 publications

References 73 publications

Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish

Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish

Multiple, solid evidence support that Austropotamobius italicus is not native to Spain

Money Kills Native Ecosystems: European Crayfish as an Example

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