Update on the diagnosis of parasitic and fungal infections

Fréalle, Émilie; Valot, Stéphane; Piarroux, Renaud; Menotti, Jean; Lachaud, Laurence; Persat, Florence; Izri, Arezki; Villard, Odile; Yera, Hélène; Dannaoui, Éric; Morio, Florent; Houzé, Sandrine

doi:10.1684/abc.2020.1554

Cited by 4 publications

(4 citation statements)

References 61 publications

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“…Conventional mycological diagnosis relies on the macroscopic and microscopic assessment of fresh and culture samples 122,123 . Direct examination of fresh samples aims at recognizing characteristic features, such as fungal hyphae.…”

Section: Overview Of Diagnostic Tools For Fungal Hypersensitivity Dis...mentioning

confidence: 99%

“…112,122 Conventional mycological diagnosis relies on the macroscopic and microscopic assessment of fresh and culture samples. 122,123 Direct examination of fresh samples aims at recognizing characteristic features, such as fungal hyphae. Inoculation on fungal growth media, followed by 5-7 days of incubation yields colonies further identified at the species level using MALDI-TOF Mass Spectrometry and a specialized fungal reference spectra database.…”

Section: Mycologymentioning

confidence: 99%

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Fungal exposome, human health, and unmet needs: A 2022 update with special focus on allergy

Vitte

Michel

Malinovschi

et al. 2022

Allergy

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Humans inhale, ingest, and touch thousands of fungi each day. The ubiquity and diversity of the fungal kingdom, reflected by its complex taxonomy, are in sharp contrast with our scarce knowledge about its distribution, pathogenic effects, and effective interventions at the environmental and individual levels. Here, we present an overview of salient features of fungi as permanent players of the human exposome and key determinants of human health, through the lens of fungal allergy and other fungal hypersensitivity reactions. Improved understanding of the fungal exposome sheds new light on the epidemiology of fungal‐related hypersensitivity diseases, their immunological substratum, the currently available methods, and biomarkers for environmental and medical fungi. Unmet needs are described and potential approaches are highlighted as perspectives.

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Section: Overview Of Diagnostic Tools For Fungal Hypersensitivity Dis...mentioning

confidence: 99%

Section: Mycologymentioning

confidence: 99%

Fungal exposome, human health, and unmet needs: A 2022 update with special focus on allergy

Vitte

Michel

Malinovschi

et al. 2022

Allergy

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“…Conventional mycological diagnosis relies on the macroscopic and microscopic assessment of fresh and culture samples (96,97). Direct examination of fresh samples aims at recognizing characteristic features, such as fungal hyphae.…”

Section: V21 Mycologymentioning

confidence: 99%

Fungal exposome, human health and unmet needs: a 2022 update with special focus on allergy

Vitte¹,

Michel²,

Malinovschi

et al. 2022

Preprint

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Humans inhale, ingest and touch thousands of fungi each day. The ubiquity and diversity of the fungal kingdom are in sharp contrast with their complex and relatively blurred taxonomy and scarce knowledge about their distribution, pathogenic effects, and effective interventions at the environmental and individual levels. Here, we present an overview of salient features of fungi as permanent players of the human exposome and key determinants of human health. Improved understanding of the fungal exposome sheds new light on the epidemiology of fungal-related diseases, their immunological substratum, the currently available methods, and biomarkers for environmental and medical fungi. Unmet needs are described and potential approaches are highlighted as perspectives.

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“…Detection methods such as 1,3-beta-D-glucan assay (G test) [ 4 ], microscopic examination [ 5 ], culture methods [ 6 ], histopathology [ 7 ], serology [ 8 ], and molecular biology [ 9 ] have been used in diagnosis of fungal infections. Traditional culture methods are widely used to characterize pathogenic fungi including Candida spp.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of pathogenic fungi from coastal population with respiratory infections using microfluidic chip technology

Yao,

He,

Deng

et al. 2024

BMC Infect Dis

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Background Currently, culture methods are commonly used in clinical tests to detect pathogenic fungi including Candida spp. Nonetheless, these methods are cumbersome and time-consuming, thereby leading to considerable difficulties in diagnosis of pathogenic fungal infections, especially in situations that respiratory samples such as alveolar lavage fluid and pleural fluid contain extremely small amounts of microorganisms. The aim of this study was to elucidate the utility and practicality of microfluidic chip technology in quick detection of respiratory pathogenic fungi. Methods DNAs of clinical samples (mainly derived from sputa, alveolar lavage fluid, and pleural fluid) from 64 coastal patients were quickly detected using microfluidic chip technology with 20 species of fungal spectrum and then validated by Real-time qPCR, and their clinical baseline data were analyzed. Results Microfluidic chip results showed that 36 cases infected with Candida spp. and 27 cases tested negative for fungi, which was consistent with Real-time qPCR validation. In contrast, only 16 cases of fungal infections were detected by the culture method; however, one of the culture-positive samples tested negative by microfluidic chip and qPCR validation. Moreover, we found that the patients with Candida infections had significantly higher rates of platelet count reduction than fungi-negative controls. When compared with the patients infected with C. albicans alone, the proportion of males in the patients co-infected with multiple Candidas significantly increased, while their platelet counts significantly decreased. Conclusions These findings suggest that constant temperature amplification-based microfluidic chip technology combined with routine blood tests can increase the detection speed and accuracy (including sensitivity and specificity) of identifying respiratory pathogenic fungi.

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Update on the diagnosis of parasitic and fungal infections

Cited by 4 publications

References 61 publications

Fungal exposome, human health, and unmet needs: A 2022 update with special focus on allergy

Fungal exposome, human health, and unmet needs: A 2022 update with special focus on allergy

Fungal exposome, human health and unmet needs: a 2022 update with special focus on allergy

Rapid detection of pathogenic fungi from coastal population with respiratory infections using microfluidic chip technology

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