Updated description of Paratylenchus lepidus Raski 1975 and P. minor Sharma, Sharma and Khan, 1986 by integrating molecular and ultra-structural observations

Abstract

Two populations of Paratylenchus lepidus and P. minor were detected in the rhizosphere of Elaeocarpus sp. and Chinese red pine from Taizhou and Hangzhou, Zhejiang Province, China. Previously, P. lepidus has been reported from China whereas P. minor was originally described from India decades ago in the rhizosphere of peach but was never reported thereafter. In this study, both species were characterized morphologically and molecularly coupled with SEM observations. Morphologically, both species have four incisures in the lateral field, vulval present (SEM observations), stylet less than 30 μ m long and cephalic region without submedian lobes. Phylogenetically, both species grouped with paratylenchid species having short stylets. Both species can be differentiated from each other by the shape of lip region (rounded in P. lepidus and narrow truncated in P. minor) and tail terminus (pointed in P. lepidus and a broadly rounded in P. minor) and several morphomemtrical values.The study provided an updated description of P. lepidus and P. minor and a first record of P. minor from China. In addition, both species are the first paratylenchid species recorded from Elaeocarpus sp. and Pinus tabuliformis, respectively.

First report of Paratylenchus lepidus Raski, 1975 associated with green tea (Camellia sinensis (L.) Kuntze) in Vietnam

“…Morphology of P. lepidus from green tea in Vietnam is in agreement with the description of the type population ( Raski, 1975 ) with small variations in measurements, however, these variations can be seen from the type population and other populations ( Maria et al, 2019 ). In this study, molecular identification is in agreement with morphological identification to support the presence of P. lepidus in Vietnam.…”

DNA Barcoding and Morphometry Reveal Further Cryptic Bio-Diversity within the Pin Nematode Genus Paratylenchus (Nematoda: Tylenchulidae)

“…D2-D3 of P. nainianus (ON873217-ON873222) showed a low similarity with P. pedrami from Spain (MW798283-MW798285) being 83.1% (118–119 bp,16 indels difference) [ 4 ], and P. leptos from Ethiopia (MW413646-MW413652) with an 83.1% similarity (120 bp, 31 indels difference) [ 3 ]. ITS from P. nainianus (ON873186-ON873189) showed a scarce similarity (sequence query coverage less than 76%) with P. minor from China (MK660189) being 84.6% (79bp, 26 indels) [ 38 ] and 84.6% similar to Paratylenchus sp. BC (KT258979, 79 bp, 26 indels).…”

Integrative Taxonomy and Molecular Phylogeny of the Plant-Parasitic Nematode Genus Paratylenchus (Nematoda: Paratylenchinae): Linking Species with Molecular Barcodes

“…The new sequences for each gene (D2-D3 of 28S, ITS, 18S rRNA and COI ) were aligned using Clustal X 1.83 [ 104 ] with their corresponding published gene sequences [ 22 , 24 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. Outgroup taxa for each dataset were chosen based on previously published data [ 105 ].…”

“…by using 58 28S rRNA and 40 ITS rRNA gene sequences. Several other studies provided additional molecular characterisations, phylogenetic analyses and descriptions of new Paratylenchus species [ 14 , 25 , 32 , 33 , 34 , 35 , 36 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. In a study by Hesar et al [ 24 ], 28S rRNA and ITS rRNA gene sequences of several Paratylenchus spp.…”