“…For the XAPT phylogeny, XAPT orthologues from a wide range of tracheophyte species were extracted from our recent phylogeny of GT47-A enzymes (Yu et al ., 2022), which contained protein sequences from Plaza Dicots 4.5, Plaza Monocots 4.5, and Plaza Gymnosperms 1.0 (Van Bel et al ., 2018), as well as from several independent genomes. To obtain further XAPT orthologues from plants in the Myrtales order, the genomes of Metrosideros polymorpha (Izuno et al, 2016; Izuno et al, 2019), Rhodamnia argantea , Syzygium oleosum , and Punica granatum (all available from NCBI Genome, https://www.ncbi.nlm.nih.gov/genome/) and the transcriptome of Oenothera rosea (Carpenter et al, 2019; One Thousand Plant Transcriptomes, 2019), were searched using TBLASTN (Altschul et al ., 1990) using At XAPT1 as a query with a cut-off at E = 1 × 10 -125 . Sequences were aligned using MUSCLE (Edgar, 2004a; Edgar, 2004b) and truncated to the GT47 domain.…”