Updates on Legume Genome Sequencing

Ha, Jung Min; Lee, Suk-Ha

doi:10.1007/978-1-0716-0235-5_1

Cited by 8 publications

(6 citation statements)

References 65 publications

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“…Subsequently, molecular marker technologies facilitated the identification of causative virus-disease-resistant genomic regions/QTL in various grain legumes using biparental QTL analysis and genome-wide association studies (GWAS). Decoding various grain legume genome sequences enabled the discovery of novel single nucleotide sequence (SNP) markers for GWAS to explore marker-trait associations/haplotypes for disease resistance at the whole-genome level (for details see Ha and Lee, 2020 ). Whole-genome resequencing and the availability of pangenomes in various legumes and viruses offer novel opportunities to explore presence/absence variations, structural variations conferring viral disease resistance, and novel resistance (R) gene(s) and virulence gene(s) across the whole genome ( Zhao et al., 2020 ; Jha et al., 2022b ).…”

Section: Introductionmentioning

confidence: 99%

Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration

et al. 2023

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Grain legumes play a crucial role in human nutrition and as a staple crop for low-income farmers in developing and underdeveloped nations, contributing to overall food security and agroecosystem services. Viral diseases are major biotic stresses that severely challenge global grain legume production. In this review, we discuss how exploring naturally resistant grain legume genotypes within germplasm, landraces, and crop wild relatives could be used as promising, economically viable, and eco-environmentally friendly solution to reduce yield losses. Studies based on Mendelian and classical genetics have enhanced our understanding of key genetic determinants that govern resistance to various viral diseases in grain legumes. Recent advances in molecular marker technology and genomic resources have enabled us to identify genomic regions controlling viral disease resistance in various grain legumes using techniques such as QTL mapping, genome-wide association studies, whole-genome resequencing, pangenome and ‘omics’ approaches. These comprehensive genomic resources have expedited the adoption of genomics-assisted breeding for developing virus-resistant grain legumes. Concurrently, progress in functional genomics, especially transcriptomics, has helped unravel underlying candidate gene(s) and their roles in viral disease resistance in legumes. This review also examines the progress in genetic engineering-based strategies, including RNA interference, and the potential of synthetic biology techniques, such as synthetic promoters and synthetic transcription factors, for creating viral-resistant grain legumes. It also elaborates on the prospects and limitations of cutting-edge breeding technologies and emerging biotechnological tools (e.g., genomic selection, rapid generation advances, and CRISPR/Cas9-based genome editing tool) in developing virus-disease-resistant grain legumes to ensure global food security.

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Section: Introductionmentioning

confidence: 99%

Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration

et al. 2023

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“…Phaseolus vulgaris L. (the common bean) is in the practical and economical crop group used to solve nutritional problems and eliminate protein deficits in the daily diet [ 1 ]. The common bean, one of the most widely grown edible legumes in the world, contains high amounts of protein (18–24%), carbohydrates (56%), and vitamins A, B, and C [ 2 , 3 , 4 , 5 ]. It is also rich in iron (Fe) and zinc (Zn) elements, which are defined as trace elements necessary for human health [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…This study identified members of the MTP gene family in the common bean genome. In addition, it aimed to determine the expression levels of PvMTP (4,5,12) genes and evaluate Fe and Zn accumulation in common bean pods and roots.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of MTP (Metal Ion Transport Proteins) Genes in the Common Bean

Yilmaz,

Özer,

Baloch

et al. 2023

Plants

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MTP/CDF carriers, called metal ion transport proteins, act as substrates for the transmission of micronutrients such as iron (Fe), zinc (Zn), and manganese (Mn) to membrane carriers in plants. In this study, genome-wide analysis of the MTP gene family in the common bean genome, expression analysis of the PvMTP4, PvMTP5, and PvMTP12 genes after Fe and Zn treatments, and the effects of Fe and Zn applications on iron and zinc content were investigated. This study used common bean genotypes assumed to have high or low Fe and Zn accumulation ability. PvMTP genes were defined as containing conserved catalytic domains with molecular weights and protein lengths ranging from 41.35 to 91.05 kDa and from 369 to 813 amino acids (aa), respectively. As a result of the phylogenetic analysis, three main clusters containing seven subgroups were formed. In this study, the first characterization of the MTP gene family of beans was performed, and the responses of three different PvMTP genes in the Zn-CDF group to Fe and Zn applications were revealed. The obtained findings are thought to constitute pioneering resources for future research on common bean biofortification studies, plant breeding related to Fe and Zn, and the functional characterization of the MTP gene family.

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“…angulari, Phaseolus vulgaris L., Cicer arietinum, Arachis hypogaea, Astragalus membranaceus var. mongholicus, Hedysarum polybotrys [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…При анализе полученных данных хотелось бы отметить, что наблюдаемая корреляция на примере представителей рода Hedysarum позволяет не только установить степень филогенетического родства различных таксонов [8,9,[40][41][42][43][44][45], но и представить совокупность хемотаксономических признаков, характерных для определенных видов на примере семейства Fabaceae, что в дальнейшем может быть использовано при проведении фармакогностических исследований и разработке нормативной документации на лекарственное растительное сырье.…”

Section: Introductionunclassified

Use of Sequencing Methods for Species Identification Exemplified by Phylogenetic Relationships Within Genus Hedysarum L.

Imachueva

Serebryanaya

Мачс

et al. 2021

Farm. farmakol. (Pâtigorsk)

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At the moment, a relevant objective in pharmacognosy, is the use of all kinds of the DNA analysis methods for identifying plant materials, detecting counterfeits, genetically modified crops and products.The aim of the research is to study the possibility of using molecular genetic research methods in the analysis of the genus Hedysarum L., for the identification of medicinal plant materials. This article presents the results of the application of molecular genetic research methods in the analysis of the genus Hedysarum L. in the flora of the North Caucasus.Materials and methods. The study material was the samples of the genus Hedysarum L. species collected in the North Caucasus: Hedysarum caucasicum M. Bieb. (in the fruiting phase in the territory of the Karachay-Cherkess Republic); Hedysarum grandiflorum Pall. (in the fruiting phase in the Volgograd region); Hedysarum daghestanicum Rupr. ex Boiss. (in the flowering phase in the Republic of Dagestan). Sequencing of the ITS1-5.8S-ITS2 marker region of gene 5.8S by the RNA ribosome was carried out according to the Sanger method on the AbiPrism 3130 genetic analyzer at the laboratory of biosystematics and cytology of Komarov Botanical Institute of the Russian Academy of Sciences.Results. Based on a comparative study of the marker region of the nuclear ribosomal gene 5.8S rRNA, marker nucleotide substitutions of Hedysarum caucasicum M. Bieb., Hedysarum daghestanicum Rupr. ex Boiss., Hedysarum grandiflorum Pall, have been identified. The most probable secondary structure of 5.8S rRNA has been constructed. It has been shown that based on the analysis performed, it is possible to predict additional raw material sources of mangiferin and other groups of xanthones using the molecular data exemplified by the Obscura section.Conclusion. Based on the data obtained, it can be concluded that the morphological classification of the genus Hedysarum L. can be confirmed within the Obscura section.

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Updates on Legume Genome Sequencing

Cited by 8 publications

References 65 publications

Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration

Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration

Genome-Wide Identification and Expression Analysis of MTP (Metal Ion Transport Proteins) Genes in the Common Bean

Use of Sequencing Methods for Species Identification Exemplified by Phylogenetic Relationships Within Genus Hedysarum L.

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