Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

Hoshino, Yumi

doi:10.1002/rmb2.12245

Cited by 27 publications

(19 citation statements)

References 107 publications

(206 reference statements)

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“…Mitochondria play a key role in adenosine triphosphate (ATP) generation for oocyte and embryonic development [ 41 ]. It is well known that mitochondria distribution is a dynamic process, and it is an important indicator of oocyte quality [ 42 ]. For example, a uniform, granulated distribution of active mitochondria in the process of oocyte maturation and also in the early embryo-specific period is essential for the normal embryo development [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

García-Martínez

Vendrell-Flotats

Martínez-Rodero

et al. 2020

IJMS

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This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM.

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Section: Discussionmentioning

confidence: 99%

Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

García-Martínez

Vendrell-Flotats

Martínez-Rodero

et al. 2020

IJMS

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“…Ovarian reserve is a key determinant of female fecundity and hence a reduction in the number of primordial follicles available in early adulthood is highly likely to be associated with an early decline in fertility (35). Fecundity in later life relates to both oocyte quality and quantity; however, there is, as yet, no well-established method of reliably predicting oocyte quality (36). Hence, there is a reliance on occyte quantity.…”

Section: Discussionmentioning

confidence: 99%

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next‐generation adult rats

et al. 2019

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Chronic fetal hypoxia is a common complication observed in human pregnancy, impacting pregnancies across global contexts. Exposure to chronic intrauterine hypoxia has major short- and long-term consequences for offspring health. However, the impact of chronic gestational hypoxia on female reproductive system development is unknown. We aimed to understand the impact of exposure to chronic fetal hypoxia on the developing female reproductive system. Wistar rat dams underwent normoxia (21%) or hypoxia (13%) during pregnancy. Postnatally, all female offspring were maintained in normoxic conditions into early adulthood. Female rats exposed to chronic gestational hypoxia (13%) during their intrauterine development had decreased ovarian primordial follicular reserve compared to controls ( P < 0.05). Adult females who had been exposed to chronic fetal hypoxia had significantly reduced somatic ovarian telomere length ( P < 0.05) and reduced ovarian protein expression of KU70, a critical component of the DNA-activated protein kinase repair complex ( P < 0.01). Gene expression of NADPH oxidase 2–mediated oxidative stress markers was increased ( P < 0.05). Exposure to chronic hypoxia during fetal development leads to accelerated aging of the somatic ovary and decreased ovarian reserve in adulthood. Ovarian aging is highly sensitive to gestational hypoxia, with implications for future fertility in next-generation offspring of high-risk pregnancies.—Aiken, C. E., Tarry-Adkins, J. L., Spiroski, A.-M., Nuzzo, A. M., Ashmore, T. J., Rolfo, A., Sutherland, M. J., Camm, E. J., Giussani, D. A., Ozanne, S. E. Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next-generation adult rats.

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“…Oocyte competence is fundamental for the formation of a gamete with full embryo and implantation potential. Oocyte competence is acquired during in vivo maturation inside the ovarian follicle, in association with local factors, brain hormones and the surrounding follicular cells, being accomplished in an integrated mode at cytoplasmic and nuclear levels (Keef et al, 2015;Conti and Franciosi, 2018;Hoshino, 2018). Regarding cytoplasmic maturation, the oocyte accumulates RNA molecules, proteins, substrates and nutrients, and calcium signalling stores (Coticchio et al, 2015;Ferrer-Buitrago et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Stereological study of organelle distribution in human oocytes at metaphase I

Coelho

Luís

Oliveira

et al. 2020

Zygote

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Summary We have previously presented a stereological analysis of organelle distribution in human prophase I oocytes. In the present study, using a similar stereological approach, we quantified the distribution of organelles in human metaphase I (MI) oocytes also retrieved after ovarian stimulation. Five MI oocytes were processed for transmission electron microscopy and a classical manual stereological technique based on point-counting with an adequate stereological grid was used. Kruskal–Wallis and Mann–Whitney U-tests with Bonferroni correction were used to compare the means of relative volumes (Vv) occupied by organelles. In all oocyte regions, the most abundant organelles were mitochondria and smooth endoplasmic reticulum (SER) elements. No significant differences were observed in Vv of mitochondria, dictyosomes, lysosomes, or SER small and medium vesicles, tubular aggregates and tubules. Significant differences were observed in other organelle distributions: cortical vesicles presented a higher Vv (P = 0.004) in the cortex than in the subcortex (0.96% vs 0.1%) or inner cytoplasm (0.96% vs 0.1%), vesicles with dense granular contents had a higher Vv (P = 0.005) in the cortex than in the subcortex (0.1% vs 0%), and SER large vesicles exhibited a higher Vv (P = 0.011) in the inner cytoplasm than in the subcortex (0.2% vs 0%). Future stereological analysis of metaphase II oocytes and a combined quantitative data of mature and immature oocytes, will enable a better understanding of oocyte organelle distribution during in vivo maturation. Combined with molecular approaches, this may help improve stimulation protocols and in vitro maturation methods.

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Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

Cited by 27 publications

References 107 publications

Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming

Chronic gestational hypoxia accelerates ovarian aging and lowers ovarian reserve in next‐generation adult rats

Stereological study of organelle distribution in human oocytes at metaphase I

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