UPLC–MS/MS simultaneous determination of seven active ingredients of Yaobitong capsule in rat plasma and its integrated pharmacokinetic application

Abstract

A reliable and sensitive UPLC–MS/MS method was first established and validated for the simultaneous determination of seven active ingredients of Yaobitong capsule in rat plasma: ginsenoside Rg1, ginsenoside Rb1, osthole, tetrahydropalmatine, paeoniflorin, albiflorin, and ferulic acid. And this method was further applied for the integrated pharmacokinetic study of Yaobitong capsule in rats after oral administration. Plasma samples (100 μL) were precipitated with 300 μL of methanol using carbamazepine as internal standard. Chromatographic separation was achieved using an Aquity UPLC BEH C18 column (100 × 2.1 mm, 1.7 μm), with the mobile phase consisting of 0.1% formic acid and acetonitrile. The method was validated using a good linear relationship (r ≥ 0.991), and the lower limit of quantification of the analytes ranged from 0.5 to 40 ng/mL. In the integrated pharmacokinetic study, the weight coefficient was calculated by the ratio of AUC0–∞ of each component to the total AUC0–∞ of the seven active ingredients. The integrated pharmacokinetic parameters Cmax, Tmax, and t1/2 were 81.54 ± 9.62 ng/mL, 1.00 ± 0.21 h, and 3.26 ± 1.14 h, respectively. The integration of pharmacokinetic parameters showed a shorter t1/2 because of fully considering the contribution of the characteristics of each active ingredient to the overall pharmacokinetics.

Efficacy and safety of Yaobitong capsule for acute lumbar disc herniation: A protocol for a multi-center randomized controlled trial

“…Recent studies on the determination of rat plasma components found that the Yaobitong capsule mainly contains ginsenoside Rg1, ginsenoside Rb1, osthol, tetrahydropalmatine, paeoniflorin, paeoniflorin, and ferulic acid, among which the content of ginsenoside from Panax notoginseng is the highest,in recent years, Ginsenoside Rg1 and ginsenoside Rb1 are considered to be effective components for the treatment of LDH. [ 34 ] Previously, several randomized controlled trials showed that oral Yaobitong capsule could significantly reduce VAS score, IL-1β and TNF- α in serum, the total effective rate of LDH is more than 95%. [ 35 – 37 ] However, the intervention of these trials adopted the scheme of Yaobitong combined with other therapies (e.g., traction, acupuncture).…”

Simultaneous determination and pharmacokinetics study of three triterpenoid saponins in rat plasma by ultra‐high‐performance liquid chromatography tandem mass‐spectrometry after oral administration of Astragalus Membranaceus leaf extract

“…The sample processing methods and mobile phase were optimized to improve peak shape, stabilize retention time, increase the signal response of analytes, and shorten run time [15][16][17]. In this study, the influence of different mobile phase combinations (methanol-water, acetonitrile-water) and added buffers (formic acid, acetic acid, ammonium formate, and ammonium acetate) on the chromatographic behavior of the target compound was investigated.…”

Effects of Borneol on the Release of Compound Danshen Colon-Specific Osmotic Pump Capsule In Vitro and Pharmacokinetics Study in Beagle Dogs

“…However, the C max and AUC 0 → 36 h of SAB, Tan IIA, R 1 , Rg 1, and Re in the formulation with borneol was mainly higher than those lacking borneol, except for Rb 1 and Rd. We use an approach of self-defined weighting coefficient to describe the holistic pharmacokinetic properties of multiple components (41,42) In conclusion, the COPC as the delivery system can first be capable of delaying the drug release and prolonging the drug plasma concentrations. In contrast, borneol in the formulation specifically increased the body exposures of SAB, Tan IIA, R 1 , Rg 1, and Re.…”