UPLC Q-TOF/MS-Based Metabolic Profiling of Urine Reveals the Novel Antipyretic Mechanisms of Qingkailing Injection in a Rat Model of Yeast-Induced Pyrexia

Gao, Xiaoyan; Guo, Mengping; Peng, Long; Zhao, Baosheng; Su, Jiankun; Liu, Haiyu; Zhang, Li; Xu, Bo; Qiao, Yanjiang

doi:10.1155/2013/864747

Cited by 33 publications

(24 citation statements)

References 43 publications

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“…These characteristics suggested a network with a small‐world property. This property demonstrated that alterations of a few important metabolites would trigger a more severe impact than changes occurring in marginal or relatively isolated positions (Gao et al, ).…”

Section: Resultsmentioning

confidence: 99%

Combined metabolomic and correlation networks analyses reveal fumarase insufficiency altered amino acid metabolism

Hou

Liu

et al. 2018

Biomedical Chromatography

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Fumarase catalyzes the interconversion of fumarate and l-malate in the tricarboxylic acid cycle. Fumarase insufficiencies were associated with increased levels of fumarate, decreased levels of malate and exacerbated salt-induced hypertension. To gain insights into the metabolism profiles induced by fumarase insufficiency and identify key regulatory metabolites, we applied a GC-MS based metabolomics platform coupled with a network approach to analyze fumarase insufficient human umbilical vein endothelial cells (HUVEC) and negative controls. A total of 24 altered metabolites involved in seven metabolic pathways were identified as significantly altered, and enriched for the biological module of amino acids metabolism. In addition, Pearson correlation network analysis revealed that fumaric acid, l-malic acid, l-aspartic acid, glycine and l-glutamic acid were hub metabolites according to Pagerank based on their three centrality indices. Alanine aminotransferase and glutamate dehydrogenase activities increased significantly in fumarase deficiency HUVEC. These results confirmed that fumarase insufficiency altered amino acid metabolism. The combination of metabolomics and network methods would provide another perspective on expounding the molecular mechanism at metabolomics level.

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Section: Resultsmentioning

confidence: 99%

Combined metabolomic and correlation networks analyses reveal fumarase insufficiency altered amino acid metabolism

Hou

Liu

et al. 2018

Biomedical Chromatography

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“…The global, unbiased metabolic profiling platform was based on a Waters ACQUITY Ultra Performance Liquid Chromatography (UPLC) system coupled to a Xevo G2-Q-TOF/MS. This platform was described in detail in our previous publication [ 25 ]. In brief, 200 μ L of each serum sample was thawed on ice and extracted by methanol protein precipitation.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis

Liu

Guo

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Objective. Berberine has been used to treat nonalcoholic steatohepatitis (NASH), which has been addressed in many studies. In this study, we investigated the molecular pharmacology mechanisms of berberine using metabolomic techniques. Methods. Sprague-Dawley rats were randomly divided into three groups (10 rats in each group): (i) normal control group; (ii) high-fat diet- (HFD-) induced NASH model group; and (iii) HFD berberine-treated group (i.d. 200 mg/kg). The handling procedure lasted eight weeks. Then, UPLC-Q-TOF/MS techniques coupled with histopathology and biochemical analyses were adopted to explore the mechanisms of berberine on the protective effects against NASH. Key Findings. (i) According to conventional test results, berberine treatment plays a fighting role in HFD-induced NASH due to its beneficial effects against insulin resistance, inflammation, and lipid metabolism. (ii) Based on UPLC-Q-TOF/MS techniques, metabolic profiles that involved sphingomyelin (SM), phosphatidylcholine (PC), lysophosphatidylcholine (LysoPC), 13-hydroperoxy-9, 11-octadecadienoic acid (13-HpODE), eicosatrienoic acid, docosatrienoic acid, and eicosenoic acid could provide potential metabolic biomarkers to address the pharmacological mechanisms of berberine. Conclusions. The parts of molecular pharmacological mechanisms of berberine for NASH treatment are related to the regulation of metabolic disruption involving phospholipid and unsaturated fatty acids in rats with NASH.

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“…The proposed algorithm was evaluated using the metabolomics data reported in [17]. To reveal the antipyretic mechanism of Qingkailing (QKL) injection in yeast-induced pyrexia rats, two groups of yeast-induced pyrexia rats were used.…”

Section: Sample Background and Preparationmentioning

confidence: 99%

Discovering Temporal Patterns in Longitudinal Nontargeted Metabolomics Data via Group and Nuclear Norm Regularized Multivariate Regression

Lin¹,

Zhang

Dai

et al. 2020

Metabolites

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Temporal associations in longitudinal nontargeted metabolomics data are generally ignored by common pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA). To discover temporal patterns in longitudinal metabolomics, a multitask learning (MTL) method employing structural regularization was proposed. The group regularization term of the proposed MTL method enables the selection of a small number of tentative biomarkers while maintaining high prediction accuracy. Meanwhile, the nuclear norm imposed into the regression coefficient accounts for the interrelationship of the metabolomics data obtained on consecutive time points. The effectiveness of the proposed method was demonstrated by comparison study performed on a metabolomics dataset and a simulating dataset. The results showed that a compact set of tentative biomarkers charactering the whole antipyretic process of Qingkailing injection were selected with the proposed method. In addition, the nuclear norm introduced in the new method could help the group norm to improve the method’s recovery ability.

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UPLC Q-TOF/MS-Based Metabolic Profiling of Urine Reveals the Novel Antipyretic Mechanisms of Qingkailing Injection in a Rat Model of Yeast-Induced Pyrexia

Cited by 33 publications

References 43 publications

Combined metabolomic and correlation networks analyses reveal fumarase insufficiency altered amino acid metabolism

Combined metabolomic and correlation networks analyses reveal fumarase insufficiency altered amino acid metabolism

Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis

Discovering Temporal Patterns in Longitudinal Nontargeted Metabolomics Data via Group and Nuclear Norm Regularized Multivariate Regression

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