UPLC-Q/TOF-MS coupled with multivariate analysis for comparative analysis of metabolomic in Dendrobium nobile from different growth altitudes

Lu, Anjing; Cao, Ligang; Tan, Daopeng; Qin, Lin; Lu, Yanliu; Zhao, Yongxia; Qian, Yongfang; Bai, Chao-jun; Yang, Jiyong; Ling, Hua; Shi, Jing‐Shan; Yang, Zhou; He, Yuqi

doi:10.1016/j.arabjc.2022.104208

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…The fragment ion m / z 236.20093 [M+H-2CH 2 ] + was produced by the loss of -2CH 2 from [M+H] + , and the latter was further cleaved -CO 2 and -CNH 3 to obtain m / z 163.11084 [M+H-C 4 H 5 O 2 N] + ; m / z 218.18927 [M+H-CH 2 O 2 ] + as a fragment ion was produced by the loss of -H 2 O and -CO from [M+H] + , and the latter was further cleaved -3CH 2 and -CH 3 to obtain m / z 176.14284 [M+H-C 4 H 4 O 2 ] + and m / z 203.16554 [M+H-C 2 H 5 O 2 ] + ; m / z 160.11208 [M+H-C 5 H 11 O 2 ] + was derived from m / z 176.14284 by the cleavage of -CH 3 , and the latter was further cleaved -CH 3 and -HCN to obtain m / z 145.10065 [M+H-C 6 H 14 O 2 ] + and m / z 133.10066 [M+H-C 6 H 12 O 2 N] + ; and the fragment ion m / z 133.10066 could drop -CH 2 to produce m / z 119.08548 [M+H-C 7 H 14 O 2 N] + ( Figure 2 ). After the relevant research literature comparison [ 7 , 8 ], DN 10 was inferred as dendrobine (C 16 H 25 NO 2 ). The molecular formula of DN 11 was C 15 H 23 NO 2 , as established by the [M+H] + peak at m / z 250.17961 (ppm −2.181).…”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents and Hypoglycemic Mechanisms of Dendrobium nobile in Treatment of Type 2 Diabetic Rats by UPLC-ESI-Q-Orbitrap, Network Pharmacology and In Vivo Experimental Verification

Zeng

Geng

et al. 2023

Molecules

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This study aimed to systematically explore the chemical constituents of D. nobile and its hypoglycemic effect by UPLC-ESI-Q-Orbitrap, network pharmacology and in vivo experiment. The chemical constituents of D. nobile were qualitatively analyzed, and the hypoglycemic compounds were quickly identified. Network pharmacological analysis and molecular docking technique were applied to assist in the elucidation of the hypoglycemic mechanisms of D. nobile. A type 2 diabetic mellitus (T2DM) rat model was established using the HFD and STZ method for in vivo experimental verification, and these T2DM rats were treated with D. nobile extract and D. nobile polysaccharide for two months by gavage. The results showed that a total of 39 chemical constituents of D. nobile, including alkaloids, bibenzyls, phenanthrenes and other types of compounds, were identified. D. nobile extract and D. nobile polysaccharide could significantly ameliorate the body weight, hyperglycemia, insulin resistance, dyslipidemia and morphological impairment of the liver and pancreas in the T2DM rats. α-Linolenic acid, dihydroconiferyl dihydro-p-coumarate, naringenin, trans-N-feruloyltyramine, gigantol, moscatilin, 4-O-methylpinosylvic acid, venlafaxine, nordendrobin and tristin were regarded as the key hypoglycemic compounds of D. nobile, along with the hypoglycemic effect on the PI3K-AKT signaling pathway, the insulin signaling pathway, the FOXO signaling pathway, the improvement of insulin resistance and the AGE-RAGE signaling pathway. The Western blotting experiment results confirmed that D. nobile activated the PI3K/AKT pathway and insulin signaling pathway, promoted glycogen synthesis via regulating the expression of glycogen synthase kinase 3 beta (GSK-3β) and glucose transporter 4 (GLUT4), and inhibited liver gluconeogenesis by regulating the expression of phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6 phosphatase (G6pase) in the liver. The results suggested that the hypoglycemic mechanism of D. nobile might be associated with liver glycogen synthesis and gluconeogenesis, contributing to improving insulin resistance and abnormal glucose metabolism in the T2DM rats.

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Section: Resultsmentioning

confidence: 99%

Chemical Constituents and Hypoglycemic Mechanisms of Dendrobium nobile in Treatment of Type 2 Diabetic Rats by UPLC-ESI-Q-Orbitrap, Network Pharmacology and In Vivo Experimental Verification

Zeng

Geng

et al. 2023

Molecules

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“…All samples were processed as described previously with some modifications (Lu et al, 2022). The fresh stems of D. officinale samples were dried at 60 °C and then grounded into powder for the assay.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Instrument conditions were set as described previously with some modifications (Lu et al, 2022). Agilent 1290 Infinity II UPLC liquid chromatograph system was employed for the present secondary metabolites analysis.…”

Section: Instrument Conditionsmentioning

confidence: 99%

“…To the best of our knowledge, whether the secondary metabolites other than polysaccharides are similarly different in D. officinale from different origins has not been reported. Due to the superiorities of high resolution, good selectivity, and shorter analysis time of ultra-high performance liquid chromatography coupled with Q/TOF-MS (UPLC-Q/ TOF-MS), the method has been widely used as a powerful tool for qualitative analysis of secondary metabolites in traditional Chinese medicine (Lu et al, 2022). In the present continuing study, UPLC-Q/TOF-MS coupled with multivariate analysis were employed for comparative analysis of the secondary metabolites of D. officinale from different growing origins.…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of the secondary metabolites of Dendrobium officinale from different growing origins by UPLC-Q/TOF-MS

et al. 2023

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Dendrobium officinale is a famous traditional Chinese medicine and nourishing food in China. The active constituents in D. officinale include a variety of chemical components such as polysaccharides, bibenzyl, phenanthrenes, and flavonoids etc. Among them, polysaccharide was selected as the quality control marker for D. officinale in the Chinese Pharmacopoeia. The previous studies found that the content of polysaccharide constituents in D. officinale from different growing origins had significant differences. To the best of our knowledge, whether the secondary metabolites other than polysaccharides are similarly different in D. officinale from different origins has not been reported. In the present continuing study, UPLC-Q/TOF-MS coupled with multivariate analysis were employed for comparative analysis of the secondary metabolites of D. officinale from different growing origins. At last, 12 differential secondary metabolites of D. officinale from different origins were identified. Network pharmacological analysis results indicated that these differential secondary metabolites of D. officinale from different origins are mainly involved in neuropsychiatric related disorder diseases. This investigation will provide a valuable information for doctors and consumers in clinical practice.

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“…DSAs share a common sesquiterpene backbone primarily synthesized through two pathways, namely the mevalonate (MVA) pathway and the MEP pathway [ 13 ]. Transcriptome sequencing of D. nobile co-cultured with endophytic fungus MF23 also identified 16 genes that were potentially involved in the synthesis of the sesquiterpene backbone [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Pathway Engineering Improves Dendrobine Production in Dendrobium catenatum

Zhao,

Yang

et al. 2023

IJMS

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The sesquiterpene alkaloid dendrobine, widely recognized as the main active compound and a quality control standard of medicinal orchids in the Chinese Pharmacopoeia, demonstrates diverse biological functions. In this study, we engineered Dendrobium catenatum as a chassis plant for the production of dendrobine through the screening and pyramiding of key biosynthesis genes. Initially, previously predicted upstream key genes in the methyl-D-erythritol 4-phosphate (MEP) pathway for dendrobine synthesis, including 4-(Cytidine 5′-Diphospho)-2-C-Methyl-d-Erythritol Kinase (CMK), 1-Deoxy-d-Xylulose 5-Phosphate Reductoisomerase (DXR), 2-C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase (MCT), and Strictosidine Synthase 1 (STR1), and a few downstream post-modification genes, including Cytochrome P450 94C1 (CYP94C1), Branched-Chain-Amino-Acid Aminotransferase 2 (BCAT2), and Methyltransferase-like Protein 23 (METTL23), were chosen due to their deduced roles in enhancing dendrobine production. The seven genes (SG) were then stacked and transiently expressed in the leaves of D. catenatum, resulting in a dendrobine yield that was two-fold higher compared to that of the empty vector control (EV). Further, RNA-seq analysis identified Copper Methylamine Oxidase (CMEAO) as a strong candidate with predicted functions in the post-modification processes of alkaloid biosynthesis. Overexpression of CMEAO increased dendrobine content by two-fold. Additionally, co-expression analysis of the differentially expressed genes (DEGs) by weighted gene co-expression network analysis (WGCNA) retrieved one regulatory transcription factor gene MYB61. Overexpression of MYB61 increased dendrobine levels by more than two-fold in D. catenatum. In short, this work provides an efficient strategy and prospective candidates for the genetic engineering of D. catenatum to produce dendrobine, thereby improving its medicinal value.

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UPLC-Q/TOF-MS coupled with multivariate analysis for comparative analysis of metabolomic in Dendrobium nobile from different growth altitudes

Cited by 7 publications

References 23 publications

Chemical Constituents and Hypoglycemic Mechanisms of Dendrobium nobile in Treatment of Type 2 Diabetic Rats by UPLC-ESI-Q-Orbitrap, Network Pharmacology and In Vivo Experimental Verification

Chemical Constituents and Hypoglycemic Mechanisms of Dendrobium nobile in Treatment of Type 2 Diabetic Rats by UPLC-ESI-Q-Orbitrap, Network Pharmacology and In Vivo Experimental Verification

Comparative analysis of the secondary metabolites of Dendrobium officinale from different growing origins by UPLC-Q/TOF-MS

Metabolic Pathway Engineering Improves Dendrobine Production in Dendrobium catenatum

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