UPLC–QTOF–MS and NMR analyses of graviola (Annona muricata) leaves

Moraes, Ingrid Vieira Machado de; Ribeiro, Paulo Riceli Vasconcelos; Schmidt, Flávio Luís; Canuto, Kirley Marques; Zocolo, Guilherme Julião; Brito, Edy Sousa de; Luo, Rensheng; Richards, Kristy M.; Tran, Kevin; Smith, Robert E.

doi:10.1016/j.bjp.2015.12.001

Cited by 10 publications

(11 citation statements)

References 17 publications

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“…These results differed from those reported by others; the average annonacin content of A. triloba fruit pulp was reported to be 7.01 mg/100 g (Potts et al, 2012) compared to 375.0 to 772.4 mg/100 g in pawpaw fruit (Levine et al, 2015). The annonacin content of pawpaw fruit determined in our study (except that of RFW and RFM) was higher than that of graviola (Annona muricata) leaves (30.56 mg/100 g; Moraes et al, 2016). The relative amount of acetogenin compounds is likely to be reflected in bioassays (Pomper & Lane, 2009), with bioactive compounds, such as phenolics present at higher levels in unripe than in ripe pawpaw fruit (Kobayashi et al, 2008).…”

Section: Acetogenin Contentcontrasting

confidence: 99%

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Nam

Park

Lee

et al. 2018

Journal of Food Science

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Pawpaw (Asimina triloba) contains acetogenins that can inhibit the growth of cancer cells. In our study, we demonstrate that the antiproliferative activity is higher in unripe than in ripe fruit and depends on acetogenin content. Our results indicate that the extract of unripe pawpaw fruit has value not only as a functional food, but has therapeutic potential for the treatment of cancer as a naturally derived substance that may be less toxic than conventional chemotherapy drugs.

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Section: Acetogenin Contentcontrasting

confidence: 99%

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Nam

Park

Lee

et al. 2018

Journal of Food Science

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“…In a separate study, freshly lyophilized graviola leaves were found to have 305.6 + 28.3 mg/g-dw annonacin and 17.4 + 0.89 mg/g-dw squamocin. 31 On the other hand, a hot, aqueous infusion (tea) solubilized only 0.213% (0.65 + 0.07 mg/g-dw) of the annonacin from 2.5 g of dry leaves and too little of the squamocin to be detected. 31 The treatment of LUHMES cells with extracts from 3 of the 4 dietary supplements led to a reduced cell viability.…”

Section: Neurotoxicity Of Dietary Supplementsmentioning

confidence: 99%

Neurotoxicity of Dietary Supplements from Annonaceae Species

et al. 2015

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Dietary supplements containing plant materials of Annonaceae species (Annona muricata L., A. squamosa L., A. mucosa JACQ., A. squamosa × cherimola Mabb.) were extracted by hot, pressurized ethyl acetate and analyzed for their effect in vitro on Lund human mesencephalic neurons. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell death was determined by lactate dehydrogenase levels. Three supplements strongly decreased the cell viability at extract concentrations of 1 µg/mL, of which 1 decreased cell viability at 0.1 µg/µL. Also, strong neuronal toxicities of these supplements were found. Cell death was observed at concentrations of 10 µg/mL. The degree of toxicity was comparable to the ones found in Annonaceous fruit extracts. Two fruit pulps of Annonaceae (A. muricata and A. squamosa) showed a reduction in cell viability at lower concentrations. The fruit pulp extract of A. muricata revealed the strongest neurotoxic effect, with 67% cell death at a concentration of 1 µg/mL. A high reduction in cell viability coupled with pronounced cell death was found at 0.1 µg/mL for an Annonaceous seed extract. These results demonstrate that the intake of dietary supplements containing plant material from Annonaceae may be hazardous to health in terms of neurotoxicity.

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“…The extraction was conducted in a shaker in a thermostatic bath (TE-052 Dubnoff Fibralimentar, Tecnal, Brazil) at 80 rpm and 50 C for 15 min, and the extracts were then filtered through a Büchner funnel with a vacuum pump. The experimental conditions used (Ethanol/water concentrations, temperature and extraction time) were optimized from a previous study by our research group (Moraes et al, 2016), but the final work with optimization planning has not yet been published.…”

Section: Raw Materialsmentioning

confidence: 99%

“…Phytochemical screening of A. muricata leaves extracts revealed that they are rich in secondary metabolites such as alkaloids, saponins, terpenoids, flavonoids, coumarins and lactones, anthraquinones, tannins, cardiac glycosides, annonaceous acetogenins, phenols and phytosterols (Gavamukulya et al, 2014;Moraes et al, 2016). The high presence of phenolic compounds confers potent antioxidant capacity to the leaves and their extracts (Nawwar et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Recovery of phenolic compounds from plant sources such as graviola leaves is generally made using methanol, ethyl acetate, acetone or other organic solvents which can compromise the final application of the extracts in food or pharmaceutical industries (Azmir et al, 2013;Moraes et al, 2016). Thus, the use of ethanol, a recognized GRAS solvent (Generally Recognized as Safe), have been extensively explored for this application.…”

Section: Introductionmentioning

confidence: 99%

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Concentration of hydroalcoholic extracts of graviola (Annona muricata L.) pruning waste by ultra and nanofiltration: Recovery of bioactive compounds and prediction of energy consumption

Moraes¹,

Rabelo

Pereira

et al. 2018

Journal of Cleaner Production

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Concentrated extracts of graviola leaves, which are widely discarded during the pruning of trees in commercial crops, were obtained by different membrane concentration process. These leaves are commonly used in traditional medicine due to their pharmacological properties. In this study, hydroalcoholic extracts (with 50 or 70% of ethanol (v/v)) of graviola leaves were subjected to ultrafiltration (UF), nanofiltration (NF) and to combination of both processes (UF followed by NF). The concentrated fractions coming from these processes were analyzed for their total antioxidant capacity and total phenolic content in order to evaluate the selectivity of each concentration step. Processes performance were also evaluated for productivity (permeate fluxes) and energy consumption, which allowed the choice of the best membrane filtration system in terms of energy savings and of interest compounds retention. The combination of processes of UF and NF showed retention coefficients higher than 75% in concentration of phenolic compounds. This sequential design resulted also in higher fluxes of NF, the fluxes increased almost seven times for extracts with 50% of ethanol, and almost three times for extracts with 70% of ethanol when compared to direct processes o NF. Furthermore, the combined system of UF and NF promoted a significant reduction in the concentration time of the extracts by NF and the characterization of fouling mechanisms, which is important parameters to scale-up of this process, indicated that the strategy may increase the useful life of the membranes.

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UPLC–QTOF–MS and NMR analyses of graviola (Annona muricata) leaves

Cited by 10 publications

References 17 publications

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Neurotoxicity of Dietary Supplements from Annonaceae Species

Concentration of hydroalcoholic extracts of graviola (Annona muricata L.) pruning waste by ultra and nanofiltration: Recovery of bioactive compounds and prediction of energy consumption

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