Upmodulation of Multinucleated Cell Formation in Long-Term Human Bone Marrow Cultures by Leukaemia Inhibitory Factor (Lif)

Heymann, Dominique; Gouin, F.; Guicheux, Jérôme; Munévar, Juan Carlos; Godard, Anne; Daculsi, G.

doi:10.1006/cyto.1996.0134

Cited by 9 publications

(3 citation statements)

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“…The progenitors of these two cell types are able to fuse into either inflammatory multinucleated giant cells (for monocytes) or osteoclast-like cells (for osteoclast progenitors). 13,14 Surprisingly, very few lacunae could be observed per sample compared to the number of mononuclear cells deposited [in the present experiment, 380 lacunae for control conditions, corresponding to 3 lacunae per 100 monocytes, and about 650 lacunae for LPS-activation, corresponding to 5.4 lacunae per 100 monocytes (purity 80%)]. It is conceivable that an LPS-stimulated (and PMB-inhibited) subpopulation degrading the BCP surface constitutes the potential circulating precursors of osteoclasts, as recently proposed by Fujikawa et al 15 Indeed, these authors have shown that human mononuclear osteoclast precursors circulate in the monocyte fraction (2%) and exhibit a monocyte phenotype.…”

Section: Discussionmentioning

confidence: 99%

Polymyxin B inhibits biphasic calcium phosphate degradation induced by lipopolysaccharide-activated human monocytes/macrophages

Kimakhe¹,

Heymann²,

Guicheux³

et al. 1998

J. Biomed. Mater. Res.

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Numerous cell types, such as monocytes and osteoclasts, are involved in calcified matrix degradation. In this context, calcium-phosphate ceramics present similar degradation processes in vivo and in vitro to those found in a natural calcified substrate. As the monocyte/macrophage lineage is among the first cells to appear in ceramic implantation sites, it is a key protagonist in inflammatory reaction and biodegradation mechanisms. This study investigated the ability of human monocytes/macrophages activated by various agents [lipopolysaccharides (LPS), polymyxin B (PMB)] to degrade biphasic calcium-phosphate ceramics. PMB sulfate is a bacteriostatic antibiotic that modulates LPS-induced cell activities in vivo and in vitro. Degradation pits (about 10 microns) produced on the pellet surface by these monocytes were discrete, with well defined margins. LPS increased the degradation of calcium-phosphate ceramic (number of lacunae, mean pellet surface area degraded) in a dose-dependent manner whereas polymyxin B downmodulated it significantly. The addition of 2 micrograms/mL of polymyxin B reduced the number of degradation lacunae and the extent of degraded surface area induced by 0.1 microgram/mL LPS by 87% and 64%, respectively. Thus this cell culture system can be very useful in the study of cellular degradation of biomaterials and of the influence of therapeutic agents that may modulate these cell activities.

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Section: Discussionmentioning

confidence: 99%

Polymyxin B inhibits biphasic calcium phosphate degradation induced by lipopolysaccharide-activated human monocytes/macrophages

Kimakhe¹,

Heymann²,

Guicheux³

et al. 1998

J. Biomed. Mater. Res.

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“…Osteoclasts are also derived from human BM cells. [61][62][63][64][65][66] BM cells are heterogenous, and most of our understanding of HSC hierarchical organization results from murine studies (please see section 3). As described above, murine osteoclasts, macrophages, and DCs differentiate from MODP.…”

Section: Human Bm Ocpmentioning

confidence: 99%

Origin of Osteoclasts: Osteoclast Precursor Cells

Tsai¹,

Kaneko²,

Suh³

et al. 2023

J Bone Metab

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Osteoclasts are multinucleated bone-resorbing cells and a key player in bone remodeling for health and disease. Since the discovery of osteoclasts in 1873, the structure and function of osteoclasts and the molecular and cellular mechanisms of osteoclastogenesis have been extensively studied. Moreover, it has been well established that osteoclasts are differentiated in vitro from myeloid cells such as bone marrow macrophages or monocytes. The concept showing that osteoclasts are derived from a specific population (named osteoclast precursor cells [OCPs]) among myeloid cells has been long hypothesized. However, the specific precursor population of osteoclasts is not clearly defined yet. A growing body of work provides evidence of the developmental origin and lifespan of murine osteoclasts, particularly in vivo. Here, we review the emerging evidence that supports the existence of OCPs and discuss current insights into their identity.

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“…[43][44][45][46][70][71][72] For example, in vitro exposure of human long-term bone-marrow culture to recombinant human LIF and OSM significantly increased the number of multinucleated cells formed after three weeks of culture. 73,74 The multinucleated cells formed expressed the macrophage polykaryon phenotype and were capable of low-grade resorption in the presence of bone-marrow stromal cells. OSM reduced the resorption induced by these cells.…”

Section: Leukaemia Inhibitory Factor (Lif) Oncostatin M (Osm) and Bomentioning

confidence: 99%

gp130 CYTOKINE FAMILY AND BONE CELLS

Heymann¹,

Rousselle²

2000

Cytokine

168

114

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Upmodulation of Multinucleated Cell Formation in Long-Term Human Bone Marrow Cultures by Leukaemia Inhibitory Factor (Lif)

Cited by 9 publications

References 0 publications

Polymyxin B inhibits biphasic calcium phosphate degradation induced by lipopolysaccharide-activated human monocytes/macrophages

Polymyxin B inhibits biphasic calcium phosphate degradation induced by lipopolysaccharide-activated human monocytes/macrophages

Origin of Osteoclasts: Osteoclast Precursor Cells

gp130 CYTOKINE FAMILY AND BONE CELLS

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