Abstract. The isotopic composition of carbon in macroalgae (δ13C) is
highly variable, and its prediction is complex concerning terrestrial
plants. The determinants of δ13C macroalgal variations were
analyzed in a large stock of specimens that vary in taxa and morphology and were
collected in shallow marine habitats in the Gulf of California (GC) with
distinctive environmental conditions. A large δ13C variability
(−34.6 ‰ to −2.2 ‰) was observed.
Life-forms (taxonomy 57 %, morphology and structural organization 34 %)
explain the variability related to carbon use physiology. Environmental
conditions influenced the δ13C macroalgal values but did not
change the physiology, which is most likely inherently species-specific.
Values of δ13C were used as indicators of the presence or
absence of carbon concentrating mechanisms (CCMs) and as integrative values
of the isotope discrimination during carbon assimilation in the life cycle
macroalgae. Based on δ13C signals, macroalgae were classified
in three strategies relative to the capacity of CCM: (1) HCO3-
uptake (δ13C > −10 ‰), (2) using a
mix of CO2 and HCO3- uptake (-10<δ13C > −30 ‰), and (3) CO2 diffusive
entry (δ13C < −30 ‰). Most species
showed a δ13C that indicates a CCM using a mix of CO2 and
HCO3- uptake. HCO3- uptake is also widespread among GC
macroalgae, with many Ochrophyta species. Few species belonging to
Rhodophyta relied on CO2 diffusive entry exclusively, while calcifying
macroalgae species using HCO3- included only Amphiroa and Jania. The isotopic
signature evidenced the activity of CCM, but it was inconclusive about the
preferential uptake of HCO3- and CO2 in photosynthesis and
the CCM type expressed in macroalgae. In the study of carbon use strategies,
diverse, species-specific, and complementary techniques to the isotopic tools
are required.