Upregulated expression of HOXC8 is associated with poor prognosis of cervical cancer

Abstract

Homeobox C8 (HOXC8) is a transcription factor that has been reported to regulate numerous genes associated with tumor progression. However, its function in cervical cancer (CC) remains to be elucidated. In the present study, the expression level of HOXC8 was examined in CC tissues and cell lines using reverse transcription-quantitative polymerase chain reaction and western blot analysis. Additionally, CC cell lines were transfected with small interfering RNAs (siRNAs) to downregulate the expression of HOX8 and assess cell proliferation using Cell Counting Kit-8. The results demonstrated a significantly increased expression of HOXC8 in CC tissues and cell lines compared with non-tumor tissues, and a normal cervical cell line, respectively. Additionally, the downregulation of HOXC8, which was achieved by siRNA transfection, significantly inhibited the proliferation rate of CC cell lines. Kaplan-Meier curves demonstrated that the increased expression of HOXC8 was associated with poor prognosis of patients with CC. Additionally, univariate and multivariate analysis revealed that HOXC8 was a significant and independent predictor for overall survival of patients with CC. In conclusion, the results of the present study suggest that HOXC8 may be involved in the progression of CC and may serve as a therapeutic target for CC.

An integrated characterization of contractile, electrophysiological, and structural cardiotoxicity of Sophora tonkinensis Gapnep. in human pluripotent stem cell-derived cardiomyocytes

“…CMs were incubated in 10% CCK-8 (Dojindo, Kumamoto, Japan) that was diluted in normal culture medium at 37 °C until a visual color conversion occurred. The absorbance of each well was measured using a microplate reader (Spectrafluor, TECAN, Sunrise, Austria) at 450 nm [15]. Three individual experiments were performed.…”

RETRACTED: miR-152 inhibits the proliferation and invasion of chordoma cells by targeting HOXC8

“…[11][12][13] HOXC8 is located at chromosome 12 and plays an oncogenic role in human cancers. [14][15][16][17]21,22 Moreover, HOXC8 is regulated by miRNAs such as miR-196s in breast cancer. 17 However, the role of HOXC8 in chordoma remains undetermined.…”

“…14 Numerous studies have investigated the role of homeobox C8 (HOXC8) in human cancers, and found that its expression was dysregulated in cervical cancer, non-small cell lung cancer, breast cancer, and osteosarcoma. [15][16][17] For example, HOXC8 expression was upregulated in cervical cancer and its overexpression promoted cell proliferation. 15 Moreover, HOXC8 was revealed as a trigger for nonsmall cell lung cancer cell proliferation and migration activation through inducing transforming growth factor-b1 expression.…”

“…[15][16][17] For example, HOXC8 expression was upregulated in cervical cancer and its overexpression promoted cell proliferation. 15 Moreover, HOXC8 was revealed as a trigger for nonsmall cell lung cancer cell proliferation and migration activation through inducing transforming growth factor-b1 expression. 16 However, the molecules regulating HOXC8 expression in cancer development are not fully understood.…”

Identification of prognostic marker genes in head and neck squamous cell carcinoma: A study based on The Cancer Genome Atlas database and experimental validation

“…Consistently, abnormally poor expression of TMPRSS11B has been revealed in squamous cell carcinoma 19 . High expression of homeobox C8 ( HOXC8 ) is associated with the poor prognosis of hepatocellular carcinoma, cervical cancer, and ovarian cancer 20–22 . Additionally, abundant expression of colony‐stimulating factor 2 ( CSF2 ) is associated with the poor overall survival and disease‐free survival in colorectal cancer patients 23 .…”