Upregulated interleukins (IL-6, IL-10, and IL-13) in immunoglobulin G4-related aortic aneurysm patients

Kasashima, Satomi; Kawashima, Atsuhiro; Zen, Yoh; Ozaki, Satoru; Kasashima, Fuminori; Endo, Makoto; Matsumoto, Yasushi; Kawakami, K.

doi:10.1016/j.jvs.2016.12.140

Cited by 43 publications

(62 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pathogenesis of IgG4‐RD remains poorly understood; however, multiple immune‐mediated mechanisms, which can be triggered by autoimmunity and infectious agents, might contribute to the fibro‐inflammatory process. Type 2 helper T cells and Tregs overexpress cytokines including interleukins (IL‐4, IL‐5, IL‐10, and IL‐13) and transforming growth factor β, promoting eosinophilia and leading to increased serum IgG4 and IgE, and progression of fibrosis . We demonstrated that approximately half of the IgG4‐PL patients had an underlying allergic disease.…”

Section: Discussionmentioning

confidence: 82%

“…The patients gave informed consent prior to participation in the study. 9 Immunoreactivities of all the antibodies were confirmed with positive and negative controls. For each antibody, the number of immunopositive cells in prominently inflamed areas was counted in five different hpfs; the average number of immunopositive cells per hpf was calculated.…”

Section: Ethics Committee Approvalmentioning

confidence: 74%

“…Type 2 helper T cells and Tregs overexpress cytokines including interleukins (IL-4, IL-5, IL-10, and IL-13) and transforming growth factor β, promoting eosinophilia and leading to increased serum IgG4 and IgE, and progression of fibrosis. 1,9 We demonstrated that approximately half of the IgG4-PL patients had an underlying allergic disease. Interestingly, two patients with IgG4-PL had a history of asbestos exposure.…”

Section: Discussionmentioning

confidence: 89%

“…Immunohistochemical examination of HEP and CBPE was performed using antibodies to detect IgG (DakoCytomation, Glostrup, Denmark; polyclonal, ×1000), IgG4 (Nichirei Bioscience Inc., Tokyo, Japan; clone HP6025, ×2), c‐kit (CD117; DakoCytomation; polyclonal, clone A4502, ×800), IgE (DakoCytomation; clone A0094, ×4000) and forkhead box P3 (FOXP3; Abcam, Cambridge, UK; mouse monoclonal, clone ab20034, 236A/E7, ×100). The c‐kit, IgE, and FOXP3 antibodies were used to detect mast cells, basophils, and regulatory T cells (Tregs), respectively . Immunoreactivities of all the antibodies were confirmed with positive and negative controls.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoreactivities of all the antibodies were confirmed with positive and negative controls. For each antibody, the number of immunopositive cells in prominently inflamed areas was counted in five different hpfs; the average number of immunopositive cells per hpf was calculated . Special staining for eosinophils (eosinophil‐mast cell kit; ScyTek Laboratories Inc., Logan, UT, USA) was used to detect eosinophils; the method of cell counting for these cells was identical to that for the immunopositive cells.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Clinicopathological features of immunoglobulin G4‐related pleural lesions and diagnostic utility of pleural effusion cytology

et al. 2018

Self Cite

View full text Add to dashboard Cite

Objective Immunoglobulin (Ig)G4‐related disease is a recently described systemic immune‐mediated fibro‐inflammatory disease that frequently occurs in tumorous form. Herein, we elucidated the clinicopathological and cytological characteristics of IgG4‐related pleural lesions (PLs). Patients and methods Among 22 patients with fibro‐inflammatory PLs of idiopathic aetiology, eight cases were diagnosed as IgG4‐PL and the remaining 14 as non‐IgG4‐PL according to comprehensive diagnostic criteria for IgG4‐related disease. Cell block examination of pleural effusion (CBPE) was performed in five patients with IgG4‐PL and in six with non‐IgG4‐PL. Both groups were compared in terms of clinical presentation, laboratory data, histopathological features of resected pleura, and cytological features of pleural effusion (PE). Results PE was the most common (six patients, 75%) clinical presentation of IgG4‐PL. IgG4‐PL comparatively showed significantly more frequent concomitant allergic disease (P = .021), higher serum IgE levels (P = .012), higher adenosine deaminase levels in pleural fluid (P = .005), and rare spontaneous recovery without treatment (P = .046). The IgG4‐PL group was histologically characterised by thicker fibrous pleura, storiform fibrosis, and infiltration of regulatory T cells, eosinophils and basophils. Using CBPE, IgG4‐PL was cytologically distinct with numerous IgG4+ cells and eosinophils. The cytology of CBPE positively correlated with the histology of pleural tissue in the number of IgG4+ cells and eosinophils (R = .769 and .803, respectively). Conclusion IgG4‐PL frequently presents with PE and is histologically and cytologically characterised by abundant infiltration of IgG4+ cells and eosinophils. We believe that CBPE with immunohistochemistry/special staining could assist in the auxiliary diagnosis of IgG4‐PL.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Ethics Committee Approvalmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Clinicopathological features of immunoglobulin G4‐related pleural lesions and diagnostic utility of pleural effusion cytology

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Inflammatory features, including symptoms, increased serum interleukin-6, and C-reactive protein, in IgG4-related vascular diseases

et al. 2018

View full text Add to dashboard Cite

Immunoglobulin (IgG) 4-related diseases (IgG4-RDs) are fibro-inflammatory conditions characterized by tumorous swelling and serum IgG4 levels. Intrapelvic IgG4-RD has been subclassified according to the localization site and aortic shape as IgG4-related aortic aneurysms (IgG4-AAs), periaortitis (IgG4-PA), and retroperitoneal fibrosis (IgG4-RF). The IgG4-AA pathogenesis would involve interleukin (IL)-6 upregulation, and Th2-predominant and Treg-activated immune conditions. We characterized the features of intrapelvic IgG4-RD lesions, including presence of vascular lesions. The clinical, serological, and pathological features, including cytokines concerning Th1/2 and Treg (IL-4, IL-6, IL-10, IL-13, and interferon-gamma) of patients with IgG4-AAs (n = 24), IgG4-PA (n = 8), and IgG4-RF (n = 10) were retrospectively compared. Clinical symptoms, such as low-grade fever, abdominal/lumber pain, and anemia, were frequently detected in IgG4-AAs but rarely in IgG4-RF. Serum IL-6 and C-reactive protein (CRP) were significantly higher in IgG4-AAs and IgG4-PA than in IgG4-RF. Pathologically, IL-6+ cells were more frequently detected in IgG4-PA and IgG4-AAs than in IgG4-RF. There were no noteworthy differences in the clinical complications, white blood cell counts, serum IgE, and serum and immunopositive cells of other cytokines between the subgroups. Among IgG4-AAs and IgG4-PA, serum IL-6 and IL-6+ cells correlated with CRP, aortic diameter, and periaortic fibrosis. IgG4-AA and IgG4-PA, but not IgG4-RF, were characterized by "inflammatory" features, such as increased CRP and serum/pathological IL-6, and clinical inflammatory symptoms; thus, IgG4-AA and IgG4-PA belong to the same group as IgG4-related vascular disease. High levels of CRP and IL-6 would be hallmarks of IgG4-related vascular disease.

show abstract

Regional disturbance of the distribution of T regulatory cells and T helper cells associated with irregular-shaped germinal centers in immunoglobulin G4-related sialadenitis

et al. 2021

View full text Add to dashboard Cite

Upregulated interleukins (IL-6, IL-10, and IL-13) in immunoglobulin G4-related aortic aneurysm patients

Cited by 43 publications

References 36 publications

Clinicopathological features of immunoglobulin G4‐related pleural lesions and diagnostic utility of pleural effusion cytology

Clinicopathological features of immunoglobulin G4‐related pleural lesions and diagnostic utility of pleural effusion cytology

Inflammatory features, including symptoms, increased serum interleukin-6, and C-reactive protein, in IgG4-related vascular diseases

Regional disturbance of the distribution of T regulatory cells and T helper cells associated with irregular-shaped germinal centers in immunoglobulin G4-related sialadenitis

Contact Info

Product

Resources

About