Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

Abstract

Objectives: Colorectal cancer (CRC) is a common carcinoma of the gastrointestinal tract with high incidence and mortality worldwide. Studies have shown that long noncoding RNAs (lncRNAs) play important roles in CRC. Our purpose is to investigate the potential of serum Linc01836 as a diagnostic and prognostic marker in CRC.Methods: We evaluated the expression of Linc01836 via quantitative real-time polymerase chain reaction (qRT-PCR). The serum CEA, CA19-9, Cyfra21-1, and CA72-4 concentrations were measured by Architect I4000 SR. Receiver operating characteristic (ROC) curves were plotted to estimate the diagnostic value in CRC. Relationship between serum Linc01836 expression and clinicopathological characteristics of CRC cases was analyzed via chi-square test. The underlying mechanism of Linc01836 on the development and prognosis in CRC was predicted by bioinformatic analysis.Results: The method of qRT-PCR for Linc01836 detection was confirmed with high precision and specificity. Serum Linc01836 expression in CRC patients was significantly higher than that in healthy donors (p < 0.0001) and benign patients (p < 0.0001), and declined after resection (p < 0.01). High expression of Linc01836 was associated with histological stage (p = 0.002) and lymph node metastasis (p = 0.006). In addition, serum Linc01836 could effectively differentiate CRC patients from the healthy folks, with favorable area under the curve (AUC) of 0.809 (95% CI: 0.757–0.861, p < 0.001). What is more, the combination of serum Linc01836, CEA, and Cyfra21-1 could improve diagnostic sensitivity (92.0%). Linc01836 was averagely located in the nucleus and cytoplasm, suggesting that it might participate in CRC progression and prognosis through the crosstalk among lncRNAs, miRNAs, and mRNAs.Conclusion: Linc01836 may serve as a valuable noninvasive biomarker for population screening, early detection, and clinical surveillance of CRC.

Integrated analysis identifies oxidative stress-related lncRNAs associated with progression and prognosis in colorectal cancer

“…High expression of these 9 lncRNAs is related to good prognosis in patients with CRC. A previous study showed that Linc01836 may serve as a valuable noninvasive biomarker for the population screening, early detection, and clinical surveillance of CRC [ 33 ]. Other studies have found that AP0355.1 is closely associated with the poor prognosis of CRC and significantly correlated with OS, so it could be used as a promising biomarker for clinical outcome and therapeutic response predictions in colon cancer patients [ 34 , 35 ].…”

The Role of Extracellular Vesicles in Colorectal Cancer

“… 38 The serum level of EVs containing linc01836 expression was increased in cancer serum from CRC patients and declined after resection. 39 Additionally, high Linc01836 expression was associated with lymph node metastasis and histological stage. 39 At the same time, combined detection of Linc01836, Cyfra21-1, and CEA in serum used for diagnosis of CRC could potentially improve sensitivity.…”

“… 39 Additionally, high Linc01836 expression was associated with lymph node metastasis and histological stage. 39 At the same time, combined detection of Linc01836, Cyfra21-1, and CEA in serum used for diagnosis of CRC could potentially improve sensitivity. 39 In addition, the study showed that the expression levels of 6 serum lncRNAs (LNCV6_116109, LNCV_108266, LNCV6_98390, LNCV6_38772, LNCV6_84003 and LNCV6_98602) in CRC patients were significantly increased and had potential to be used as biomarkers for early diagnosis of CRC.…”

“… 39 At the same time, combined detection of Linc01836, Cyfra21-1, and CEA in serum used for diagnosis of CRC could potentially improve sensitivity. 39 In addition, the study showed that the expression levels of 6 serum lncRNAs (LNCV6_116109, LNCV_108266, LNCV6_98390, LNCV6_38772, LNCV6_84003 and LNCV6_98602) in CRC patients were significantly increased and had potential to be used as biomarkers for early diagnosis of CRC. 40 With the development of RNA-seq technology, scientists have identified tens of thousands of EVs containing circRNAs that can interact with miRNAs and transcription factors to regulate gene expression.…”

Long noncoding RNA SNHG15: A promising target in human cancers

“…The early and accurate diagnosis was undoubtedly crucial to prolong the lifespan of cancer patients, yet current methods do not fully meet the demand for early diagnosis ( 106 , 107 ). Numerous studies revealed that serum lncRNA could serve as an effective biomarker for cancer detection ( 108 , 109 ).…”