Upregulated miR-194-5p suppresses retinal microvascular endothelial cell dysfunction and mitigates the symptoms of hypertensive retinopathy in mice by targeting SOX17 and VEGF signaling

Wan, Qianqian; Liu, Heting; Xu, Yuxin; Zhang, Qing; Tao, Liming

doi:10.1080/15384101.2022.2119514

Cited by 2 publications

(2 citation statements)

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“…For instance, the dysregulation of miR-637 has been reported to be implicated in the progression of retinopathy in patients with hypertension and regulate retinol endothelial cell proliferation [26]. In addition, upregulated miR-194-5p has been reported to suppress the dysfunctions of retinal microvascular endothelial cells and mitigate hypertensive retinopathy symptoms in mice via binding to SOX17 and VEGF pathways [9]. Herein, the expression levels of several miRNAs showed to be signi cantly changed within the hypertensive retinopathy model than normal control, as well as in the treatment group compared with the model group, further evidencing that miRNAs might contribute to hypertensive retinopathy development and could potentially act as therapeutic targets.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have highlighted the importance of ncRNAs in hypertensive retinopathy. For instance, miRNAs, which are small ncRNAs that bind to the 3' untranslated region of target mRNAs to modulate gene expression, could regulate endothelial function, in ammation, and vascular remodeling in hypertensive retinopathy [7][8][9]. Similarly, lncRNAs that are longer than 200 nucleotides and could modulate gene expression through various mechanisms, have been shown to be differentially expressed within the retinas of hypertensive rats and may contribute to the pathogenesis of hypertensive retinopathy [10,11].…”

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confidence: 99%

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Insights into RNA transcriptome sequencing of retinal tissues in hypertensive retinopathy rat model

Wen,

Wang,

et al. 2023

Preprint

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Background Hypertensive retinopathy, a complication of systemic hypertension that affects the microvasculature of the retina which result in serious visual disorder. The aim of the present study was to investigate the differentially expressed mRNAs, miRNAs, lncRNAs, and circRNAs in a rat model of hypertensive retinopathy (spontaneously hypertensive rats, SHR) with or without treatment and to explore their potential roles, involved functions, and signaling pathways. Results Our results revealed that the expression levels of mRNAs, miRNAs, lncRNAs, and circRNAs were significantly changed in the hypertensive retinopathy models with or without drug therapy. The differentially expressed non-coding RNAs were predicted to target genes contributing to various biological processes and signaling pathways related to hypertensive retinopathy, including immune regulation, wound healing, blood vessel remodeling, and response to external stimuli. Besides, the competing endogenous RNA (ceRNA) regulatory network consisting of circRNAs and miRNAs identified potential interactions between ncRNAs and their possible roles in hypertensive retinopathy diagnosis and treatment. Conclusions The results of this study shed light on the potential effects of mRNAs, miRNAs, lncRNAs, and circRNAs on hypertensive retinopathy pathogenesis and treatment. These molecules could serve as potential biomarkers for early-stage diagnosis or therapeutic targets for hypertensive retinopathy.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Insights into RNA transcriptome sequencing of retinal tissues in hypertensive retinopathy rat model

Wen,

Wang,

et al. 2023

Preprint

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circSKA3 promotes colorectal cancer metastases through miR-1238 and methylation

et al. 2023

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Colorectal cancer (CRC) is becoming one of the most common cancers overworld, which causes a high rate of death in patients. circRNAs are non-coding RNAs(ncRNAs), which have been reported to be involved in the development of many cancers, including CRC. However, the exact mechanism that how circRNAs function through in CRC remains unclear. In this study, we firstly used GEO database and bioinformatic methods to identify the significant changed circRNAs, with circSKA3 being the most significantly upregulated circRNAs in CRC tissues. PCR results further confirmed higher expression of circSKA3 in CRC patients. CCK-8, scratch, and transwell assays indicated that circSKA3 could promote the proliferation, migration, and invasion of CRC cell lines for cell detection. Dual-luciferase assays were carried out to detect the downstream targets of circSKA3, and a binding site between circSKA3 and miR-1238 was identified and miR-1238 could also combine with YTHDF2. Overexpression of YTHDF2 rescued the decreased cell proliferation, migration, and invasion caused by miR-1238 overexpression. RIP assay further indicated that YTHDF2 could decrease the methylation of STAT5A. In summary, our study found that circSKA3 was upregulated in CRC tissues comparing with normal tissues. circSKA3 could increase the expression ofYTHDF2 through sponging miR-1238 to decrease the methylation of STAT5A, which could provide a novel target for CRC treatment.

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Upregulated miR-194-5p suppresses retinal microvascular endothelial cell dysfunction and mitigates the symptoms of hypertensive retinopathy in mice by targeting SOX17 and VEGF signaling

Cited by 2 publications

References 64 publications

Insights into RNA transcriptome sequencing of retinal tissues in hypertensive retinopathy rat model

Insights into RNA transcriptome sequencing of retinal tissues in hypertensive retinopathy rat model

circSKA3 promotes colorectal cancer metastases through miR-1238 and methylation

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