Upregulated miR-322-5p regulates cell cycle and promotes cell proliferation and apoptosis by directly targeting Wee1 in mice liver injury

He, Tong Chuan; Wang, Li; Shi, Jing; Jin, Haowei; Zhang, Kefan; Bao, Yong-Rui; Wu, Yongshuai; Cheng, Yipeng; Liu, Pengxia; Wang, Changshan

doi:10.1080/15384101.2022.2108128

Cited by 8 publications

(1 citation statement)

References 40 publications

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“…miRNAs interfere with protein translation by binding to the sequences in the 3' untranslated region (UTR) of messenger RNAs (mRNAs), reducing the stability of mRNAs or inhibiting the expression of the transcription genes (Bartel 2009). Participating in various physiological processes such as cell proliferation and apoptosis, it plays an important regulatory role in diseases and is closely related to the occurrence of various tumors (Jaszczuk et al 2022;Menon et al 2022;Tong et al 2022). Circulating miRNAs have received increasing attention in recent years as diagnostic markers for various diseases due to their convenience in monitoring (Li et With the development of sequencing technology, bioinformatics has been used to explore the pathological mechanisms of various diseases at the gene level.…”

Section: Introductionmentioning

confidence: 99%

miR-455-3p has superior diagnostic potential to PSA in peripheral blood for prostate cancer

Cen,

Feng,

et al. 2024

Preprint

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Background Prostate-specific antigen (PSA) is commonly used as a biomarker to diagnose and predict the course of prostate cancer (PCa). However, PSA detection is susceptible to changes in the physiologic environment, which may lead to some misdiagnosis. Thus, it is crucial to find a novel diagnostic marker. Methods We accessed microRNA (miRNA) expression datasets (GSE206793 and GSE112264) from the GEO database, analyzing peripheral blood samples from PCa patients. Differentially expressed miRNAs (DEmiRNAs) were identified using GEO2R. A specific miRNA, miR-455-3p, was pinpointed through rigorous analysis of clinical correlations and ROC curves. Peripheral blood samples from healthy individuals and PCa patients were subjected to qRT-PCR validation, aligning results with the GSE206793 dataset. The miRWalk database was utilized to predict downstream genes, while STRING facilitated the construction of a protein-protein interaction (PPI) network. KEGG pathway analysis enriched our understanding of potential molecular pathways. Results We found that miR-455-3p was highly expressed in the peripheral blood of PCa patients with Gleason score (GS) ≥ 8, while independent of T stage, age and PSA. ROC analysis revealed a favorable diagnostic efficacy of miR-455-3p and AUC for the two datasets was respectively 0.943 and 0.847. The qRT-PCR assay also revealed consistent results. Interestingly, the PSA levels of P1 (GS = 5 + 4) and P6 (GS = 3 + 3) were respectively 3.38 and 4.45 ng/ml, while miR-455-3p was highly expressed in both, suggesting its low misdiagnosis. The speculation was validated in GSE206793 dataset. Finally, 9 potential targets of miR-455-3p were predicted. PPI network revealed PPP2R2A, ITGB1 and CDKN1A as key nodes. KEGG pathway analysis revealed that they were enriched in various cancers, biological processes and molecular signals. Conclusion Our study identifies miR-455-3p as a promising diagnostic marker for PCa, outperforming PSA in terms of specificity and sensitivity. The robustness of miR-455-3p, coupled with its potential downstream targets and associated pathways, highlights its clinical significance for improved PCa diagnosis and management.

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Section: Introductionmentioning

confidence: 99%