Upregulating KTN1 promotes Hepatocellular Carcinoma progression

Abstract

Background: Hepatocellular carcinoma (HCC) presents a common malignant tumor worldwide. Although kinectin 1 (KTN1) is the most frequently identified antigen in HCC tissues, the detailed roles of KTN1 in HCC remain unknown. This study seeks to clarify the expression status and clinical value of KTN1 in HCC and to explore the complicated biological functions of KTN1 and its underlying mechanisms. Methods: In-house reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of KTN1 in HCC tissues. External gene microarrays and RNA-sequencing datasets were downloaded to confirm the expression patterns of KTN1. The prognostic ability of KTN1 in HCC was assessed by a Kaplan-Meier curve and a hazard ratio forest plot. The CRISPR/Cas9 gene-editing system was used to knock out KTN1 in Huh7 cells, which was verified by PCR-Sanger sequencing and western blotting. Assays of cell migration, invasion, viability, cell cycle, and apoptosis were conducted to explore the biological functions. RNA sequencing was performed to quantitatively analyze the functional deregulation in KTN1-knockout cells compared to Huh7-wild-type cells. Upregulated genes that co-expressed with KTN1 were identified from HCC tissues and were functionally annotated. Results: KTN1 expression was increased in HCC tissues (standardized mean difference [SMD] = 0.20 [0.04, 0.37]). High KTN1 expression was significantly correlated with poorer prognosis of HCC patients, and KTN1 may be an independent risk factor for HCC (pooled HRs = 1.31 [1.05, 1.64]). After KTN1-knockout, the viability, migration, and invasion ability of HCC cells were inhibited. The proportion of HCC cells in the G0-G1 phases increased after KTN1 knockout, which also elevated the apoptosis rates in HCC cells. Several cascades, including innate immune response, chemical carcinogenesis, and positive regulation of transcription by RNA polymerase II, were dramatically changed after KTN1 knockout. KTN1 primarily participated in the cell cycle, DNA replication, and microRNAs in cancer pathways in HCC tissues. Conclusion: Upregulation of KTN1 served as a promising prognosticator in HCC patients. KTN1 promotes the occurrence and deterioration of HCC by mediating cell survival, migration, invasion, cell cycle activation, and apoptotic inhibition. KTN1 may be a therapeutic target in HCC patients.

A novel model for predicting prognosis in patients with idiopathic pulmonary fibrosis based on endoplasmic reticulum stress‐related genes

“…The KTN1 gene, a member of the kinectin protein family, primarily localizes in the ER membrane and plays a key role in encoding an integral membrane protein and regulating the localization and distribution of the ER (Zheng et al, 2022). Previously, research on KTN1 focused on cancer occurrence (Li, Li, et al, 2021; Pan et al, 2021). In breast cancer, the KTN1 protein activated the NF‐κB signaling pathway, which is also intensely associated with the occurrence of pulmonary fibrosis (Gao et al, 2021; Wei et al, 2023; Zhang et al, 2022).…”

PRMT1 promotes the proliferation and metastasis of gastric cancer cells by recruiting MLXIP for the transcriptional activation of the β-catenin pathway

“…As a kinectin, KTN1 has been reported to promote hepatocellular carcinoma progression and the growth of triple-negative breast cancer. 44 , 45 In 2021, Zhenhua Ding and his colleagues made a breakthrough in their study on KTN1 function. They found that KTN1 stabilized the protein expression level of EGFR by decreasing its ubiquitination, 46 suggesting that the MALAT1-KTN1-EGFR axis promoted the development of cutaneous squamous cell carcinoma.…”