Upregulation of CD4+ T-Lymphocytes by Isomeric Mixture of Quercetin-3-O-Rutinoside and Quercetin-3-O-Robinobioside Isolated from Millettia aboensis

Ajaghaku, Daniel Lotanna; Akah, Peter A.; Ilodigwe, Emmanuel Emeka; Nduka, Sunday Odunke; Osonwa, Uduma Eke; Okoye, Festus Basden Chiedu

doi:10.1080/08820139.2018.1433201

Cited by 12 publications

(7 citation statements)

References 24 publications

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“…We further purified compounds 2, 3, 7, 9, and 10 by preparative-RP-HPLC. We identified the major isolated compounds (Figure 2) as 4-hydroxy-Nmethylproline (2), 6-β-hydroxyipolamiide (3), quercetin-3-O-robinobioside (7), hyperin (8), isorhamnetin-3-Orobinobioside (9), and 3'-O-methylhyperin (10) on the basis of the 1D and 2D NMR data and by comparison with previously reported data (Grassi et al, 2005;Sciuto et al, 1983;Ajaghaku et al, 2018;Buschi, Pomilio, 1982;Agrawal, 1989;Mendez, Bilia, Morelli, 1995). Moreover, this is the first report on the presence of quercetin-3-Orobinobioside and isorhamnetin-3-O-robinobioside in this genus.…”

Section: Resultsmentioning

confidence: 85%

Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Andrade

Alves

Costa

et al. 2022

Braz. J. Pharm. Sci.

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Adenocalymma axillarum (K.Schum.) L.G.Lohmann, synonym Memora axillaris, popularly known as "ciganinha" and "caroba-amarela", is an Angiosperm belonging to the family Bignoniaceae and to the tribe Bignonieae, which predominates in the Neotropics and comprises important neotropical forest components. This family consists of approximately 82 genera and 827 species that occur as shrubs, trees, and climbing plants (Lohmann, Taylor, 2014; Lorenzi, Souza, 2001;Olmstead et al., 2009). From a chemical viewpoint, the genus Adenocalymma is characterized by the presence of allantoin, terpenoids, diallyl di-, tri-, and tetrasulfides, and flavonoids (de

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Section: Resultsmentioning

confidence: 85%

Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Andrade

Alves

Costa

et al. 2022

Braz. J. Pharm. Sci.

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“…IgG1 isotype (Th2 immune response) is stimulated by IL‐5 and IL‐10 whereas IgG2a (Th1 immune response) is stimulated by IFN‐γ and IL‐12 . High levels of the isotypes IgG1 and IgG2a act as surrogate markers of Th2 and Th1 cell‐mediated immune responses, respectively . Data presented in Figure b,c, indicate that GSLS in combination with Se significantly increased both IgG1 and IgG2a levels, which may be explained by the stronger expression of both Th2 (IL‐5 and IL‐10) and Th1 (IFN‐γ and IL‐12) cytokines by splenocytes (Figure ), suggesting in turn that both Th1 and Th2 cell‐mediated immune responses were stimulated.…”

Section: Discussionmentioning

confidence: 87%

“…26 High levels of the isotypes IgG1 and IgG2a act as surrogate markers of Th2 and Th1 cell-mediated immune responses, respectively. 27 Data presented in Figure 2b,c, indicate that GSLS in combination with Se significantly increased both IgG1 and IgG2a levels, which may be explained by the stronger expression of both Th2 (IL-5 and IL-10) and Th1 (IFN-γ and IL-12) cytokines by splenocytes ( Figure 6), suggesting in turn that both Th1 and Th2 cell-mediated immune responses were stimulated. NK cells (cytotoxic lymphocytes) are an important component of the innate immune response to viral infections.…”

Section: Gsls and Se Confer Protection To Mice Challenged With Lethmentioning

confidence: 86%

Ginseng stem‐leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine

Maqbool

Wang

Cui

et al. 2019

Microbiology and Immunology

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Pseudorabies, a herpesvirus infection, is mainly controlled by using attenuated live vaccines. In this study, the effect of ginseng stem and leaf saponins (GSLS) in combination with selenium (Se; in the form of sodium selenite) on vaccination against attenuated pseudorabies virus (aPrV) was evaluated. It was found that GSLS and Se have an adjuvant effect and that a combination of GSLS and Se stimulates significantly enhanced immune responses than does GSLS or Se alone. Following oral administration of GSLS, mice immunized with an attenuated PrV vaccine diluted in Se‐containing physiological saline solution (PSS) provoked a significantly stronger gB‐specific serum antibodies response (IgG, IgG1 and IgG2a), enhanced lymphocyte proliferation and cytolytic activity of NK cells, along with higher production of cytokines (IFN‐γ, IL‐12, IL‐5 and IL‐10) by splenocytes. Notably, the combination of GSLS and Se conferred a much higher resistance to fPrV challenge after immunization of the mice with aPrV vaccine. This study offers convincing experimental evidence that an injection of Se with oral GSLS is a promising adjuvant combination that improves the efficacy of vaccination against PrV and deserves further study regarding improvement of responses to other animal vaccines.

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“…The same result was reported in the study of Ajaghaku et al . [ 30 ]. Furthermore, this study showed the positive effect of quercetin-3- O -rutinoside to increase the activity of CD4+ T cells in mice with toxoid tetanus.…”

Section: Discussionmentioning

confidence: 99%

“…Quercetin-3- O -rutinoside is a type of quercetin that plays a prominent immunological role in medicinal plants [ 31 ]. Quercetin can stimulate the proliferation of T-cell lymphocytes [ 30 ]. Long-term quercetin administration was reported to increase the proliferation of immune cells in the splenic organ and significantly impact the CD4+/CD8+ response to suppress the induction of the immune system by radiation [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer activity of an ethanolic extract of red okra pods (Abelmoschus esculentus L. Moench) in rats induced by N-methyl-N-nitrosourea

et al. 2022

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Background and Aim: Breast cancer is the most frequent malignancy in women. The consumption of phytochemical components from plants may play an essential role in preventing and treating this cancer. This study aimed to investigate the anti-cancer activity of an ethanolic extract of red okra pods (EEROP) in rats (Rattus norvegicus) induced by N-methyl-N-nitrosourea (MNU). Materials and Methods: The experimental animals were divided into six groups (n=5/group), namely, KN (normal control, without any treatment), K– (negative control, exposed to MNU without EEROP), K+ (positive control, exposed to MNU and Methotrexate), and the treatment Groups P1, P2, and P3 (exposed to MNU and EEROP at doses of 50, 100, and 200 mg/kg body weight [BW], respectively). Intraperitoneal delivery of MNU and EEROP oral administration was carried out for 8 weeks. After the end of treatment, the parameters of cytokines, CD4+ and CD8+ T cells, and mammary gland histology were measured. Results: The results showed that EEROP at doses of 100 and 200 mg/kg BW significantly downregulated interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, IL-17, IL-10, and tumor growth factor-β (p<0.05). In addition, doses of 200 mg/kg BW significantly increased the activity of CD4+ and CD8+ T cells, prevented the proliferation of mammary gland epithelial cells, and yielded a significantly thinner epithelium of the mammary gland (p<0.05). Conclusion: It can be concluded that EEROP was an effective anti-cancer agent by modulating the immune response. Further studies using a nanoparticle system are warranted to achieve optimal working conditions for these bioactive compounds.

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Upregulation of CD₄⁺ T-Lymphocytes by Isomeric Mixture of Quercetin-3-O-Rutinoside and Quercetin-3-O-Robinobioside Isolated from Millettia aboensis

Abstract: M. aboensis expressed strong immune-enhancing properties, which may explain its ethnopharmacological use in disease management.

Cited by 12 publications

References 24 publications

Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Ginseng stem‐leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine

Anti-cancer activity of an ethanolic extract of red okra pods (Abelmoschus esculentus L. Moench) in rats induced by N-methyl-N-nitrosourea

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