Upregulation of Cell-Surface-Associated Plasminogen Activation in Cultured Keratinocytes by Interleukin-1β and Tumor Necrosis Factor-α

Elevated plasminogen receptor expression occurs as a degradative phase event in cellular apoptosis

“…We have previously shown 12 that PLM generated on the surface of apoptotic cells remains there for more than 2 h, so it is unlikely that PLM is internalized. Several independent studies have suggested that PA might be involved in anoikis during apoptosis of epithelial cells 15 , 23 . This speculative suggestion is attractive and provides a logical explanation for the possible role of extracellular PLM.…”

“…Several independent studies have suggested that PA might be involved in anoikis during apoptosis of epithelial cells. 15,23 This speculative suggestion is attractive and provides a logical explanation for the possible role of extracellular PLM.…”

Pharmacological investigation on the wound healing effects of Radix Rehmanniae in an animal model of diabetic foot ulcer

“…As inflammation involves very complicated physiological responses, more studies should be conducted to further investigate the effects of RR extract on wound healing, i.e. cytokine secretion such as TNF and interleukin may help further assess the role of RR in modifying inflammatory responses (Bechtel et al, 1996;Theze, 1999;Grellner, 2002;Tonks et al, 2003).…”

Plasmin induces degradation and dysfunction of laminin 332 (laminin 5) and impaired assembly of basement membrane at the dermal–epidermal junction

“…55 In addition to MMPs, uPA and uPAR were reported to be synthesized and secreted in human keratinocytes exposed to UVB. 23,24 uPA, uPAR and pericellular plasmin-mediated proteolysis are also reported to be increased by proinflammatory cytokines, interleukin-1b and tumour necrosis factor-a, 56 which are known to be synthesized and secreted in UVB-exposed skin. 57 uPA is expressed predominantly in basal keratinocytes 58 and plasminogen is derived from plasma and distributed in the basal layer of the epidermis.…”

“…After washing, bound laminin 332 was detected using polyclonal antilaminin 332 antibodies as described in Materials and methods. inflammatory cytokines 56 and plasminogen is supplied from the plasma, so plasmin may be expected to be increased in the vicinity of laminin 332. In this study, we found that plasmin suppressed the assembly of BM even in the presence of MMP inhibitor, and cleaved the a3 and b3 chains of laminin 332, which reduced keratinocyte attachment and the binding of type VII collagen.…”